Long Noncoding RNA H19 Inhibits Cell Viability, Migration, and Invasion Via Downregulation of IRS-1 in Thyroid Cancer Cells

Abstract

Thyroid cancer is a common endocrine gland malignancy which exhibited rapid increased incidence worldwide in recent decades. This study was aimed to investigate the role of long noncoding RNA H19 in thyroid cancer. Long noncoding RNA H19 was overexpressed or knockdown in thyroid cancer cells SW579 and TPC-1, and the expression of long noncoding RNA H19 was detected by real-time polymerase chain reaction. The cell viability, migration, and invasion were determined by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide assay, Transwell assay, and wound healing assay, respectively. Furthermore, cell apoptosis was analyzed by flow cytometry, and expressions of some factors that were related to phosphatidyl inositide 3-kinases/protein kinase B and nuclear factor κB signal pathway were measured by Western blotting. This study revealed that cell viability and migration/invasion of SW579 and TPC-1 were significantly decreased by long noncoding RNA H19 overexpression compared with the control group (P < .05), whereas cell apoptosis was statistically increased (P < .001). Meanwhile, cell viability and migration/invasion were significantly increased after long noncoding RNA H19 knockdown (P < .05). Furthermore, long noncoding RNA H19 negatively regulated the expression of insulin receptor substrate 1 and thus effect on cell proliferation and apoptosis. Insulin receptor substrate 1 regulated the activation of phosphatidyl inositide 3-kinases/AKT and nuclear factor κB signal pathways. In conclusion, long noncoding RNA H19 could suppress cell viability, migration, and invasion via downregulation of insulin receptor substrate 1 in SW579 and TPC-1 cells. These results suggested the important role of long noncoding RNA H19 in thyroid cancer, and long noncoding RNA H19 might be a potential target of thyroid cancer treatment.

LncRNA MAFG‐AS1 facilitates the migration and invasion of NSCLC cell via sponging miR‐339‐5p from MMP15

“…After 24 h, the invaded cells were fixed using 4% paraformaldehyde and were stained using 1% crystal violet. The number of invaded cells was counted (Wang et al, ).…”

LncRNAs as Potential Therapeutic Targets in Thyroid Cancer

“…H19 mediated negative down regulation of insulin receptor by inactivation of PI3 kinase/Akt and nuclear factor Kappa B pathway. Owing to these characteristics, H19 may be potentially used as a therapeutic target for TC (Wang et al, 2017). Using qPCR, it was they demonstrated that downregulation of H19 was responsible of reduced metastatic potential in PTC and thus H19 expression could be utilized as diagnostic marker for PTC (Jiao et al, 2019).…”

Targeted inhibition of long non-coding RNA H19 blocks anaplastic thyroid carcinoma growth and metastasis

“…Liang et al reported that H19 was overexpressed in papillary thyroid carcinoma (PTC), and H19 overexpression contributes to epithelial-mesenchymal transition (EMT) [18]. However, Wang et al reported that H19 overexpression inhibited invasion and EMT in PTC cells [19], and Lan et al reported that H19 downregulation contributes to proliferation and migration in PTC cells [20]. H19 expression and its role in ATC were still unclear.…”