Long noncoding RNA FALEC inhibits proliferation and metastasis of tongue squamous cell carcinoma by epigenetically silencing ECM1 through EZH2

Jia, Bo; Xie, Tao; Qiu, Xiaoling; Sun, Xiang; Chen, Jun; Huang, Zhijie; Zheng, Xianghuai; Wang, Zhiping; Zhao, Jianjiang

doi:10.18632/aging.102094

Cited by 31 publications

(29 citation statements)

References 29 publications

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“…A change in lncRNA expression has been identified in a variety of human cancer types, including gastric cancer (11), thyroid cancer (12), hepatocellular carcinoma (13) and TScc (14). Regarding TScc, recent studies have indicated that numerous lncRNAs are abnormally expressed in this type of tumor and act as either tumor-suppressors or oncogenic RNAs (15,16). The abnormal expression of lncRNAs may contribute toward the malignancy of TScc by affecting a number of malignant characteristics (17)(18)(19).…”

Section: Introductionmentioning

confidence: 99%

Long non‑coding RNA PRNCR1 exerts oncogenic effects in�tongue squamous cell carcinoma in�vitro and in�vivo by sponging microRNA‑944 and thereby increasing HOXB5�expression

Zou

et al. 2020

Int J Mol Med

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A long non-coding RNA (lncRNA) called prostate cancer-associated non-coding RNA 1 (PRNCR1) serves crucial roles in the aggressive phenotypes of colorectal cancer and non-small cell lung cancer. However, there is little research on the expression profile, clinical value and detailed functions of PRNCR1 in tongue squamous cell carcinoma (TScc). The aim of the present study was to determine PRNCR1 expression in TScc and to examine the involvement of PRNCR1 in TScc progression. The molecular mechanisms behind the oncogenic effects of PRNCR1 in TScc cells were also investigated. PRNCR1 was revealed to be upregulated in TScc tumors and cell lines. The high PRNCR1 expression showed a significant correlation with tumor size, clinical stage, lymph node metastasis, and shorter overall survival times among patients with TScc. A PRNCR1-knockdown reduced TScc cell proliferation, migration and invasion, and increased apoptosis in vitro. Additionally, the PRNCR1-knockdown slowed down in vivo tumor growth of TScc cells. With regards to the mechanism, PRNCR1 acted as a competing endogenous RNA on microRNA-944 (miR-944) in TScc cells, and the effects of the PRNCR1-knockdown were reversed by an miR-944-knockdown. HOXB5 was validated as a direct target gene of miR-944 in TScc cells, and HOXB5 expression was found to be positively regulated by PRNCR1. Furthermore, resumption of HOXB5 expression reversed the tumor-suppressive actions of miR-944 in TScc cells. In conclusion, PRNCR1 acts as an oncogenic lncRNA in TScc through the upregulation of HOXB5 by sponging miR-944, thereby indicating a potential therapeutic target in TScc.

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Section: Introductionmentioning

confidence: 99%

Long non‑coding RNA PRNCR1 exerts oncogenic effects in�tongue squamous cell carcinoma in�vitro and in�vivo by sponging microRNA‑944 and thereby increasing HOXB5�expression

Zou

et al. 2020

Int J Mol Med

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“…Recently, growing evidence has demonstrated that lncRNAs may also have essential roles in TSCC. For example, overexpression of the lncRNA FALEC represses malignant behaviors in TSCC (6). Upregulation of the lncRNA HOTTIP in TSCC patients indicates a poor clinical prognosis (7).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Differentially Expressed Long Non-coding RNAs and the Associated TF-mRNA Network in Tongue Squamous Cell Carcinoma

et al. 2020

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Accumulating evidence indicates that long non-coding RNAs (lncRNAs) play crucial roles in tongue squamous cell carcinoma (TSCC) tumorigenesis. However, the comprehensive regulation of lncRNAs-transcription factors (TFs)-messenger RNAs (mRNAs) in TSCC remains largely unknown. The purpose of this study was to identify aberrantly expressed lncRNAs and the associated TF-mRNA network in TSCC. To explore lncRNA and mRNA expression profiles and their biological functions in TSCC, we surveyed the lncRNA and mRNA expression profiles of TSCC and adjacent tissues using next-generation RNA sequencing in six patients. Thousands of significantly differentially expressed lncRNAs (DELs) and mRNAs (DEGs) were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to demonstrate the principal functions of the significantly dysregulated lncRNAs and genes. A total of 40 DELs were screened between TSCC and adjacent non-cancerous tissues. Results obtained from GEPIA and StarBase confirmed the expression levels of nine pivotal DELs obtained in our study. Three of the nine deregulated DELs were identified to have a significant impact on the overall survival of TSCC patients, which were evaluated with GEPIA and StarBase. LncMAP was used to predict the lncRNA-TF-mRNA triplets in TSCC. Furthermore, based on these results, we established lncRNA-TF-mRNA coexpression networks for the up-and downregulated lncRNAs using Cytoscape. We also found that among the nine pivotal lncRNAs, there is limited research on the abnormally expressed lncRNAs, including RP11-54H7.4, CTD-2545M3.8, RP11-760H22.2, RP4-791M13.3, and LINC01405, in TSCC pathogenesis. This is the first study to show that RP11-54H7.4, LINC00152, and LINC01405 can be acted as a prognostic target for TSCC. Our findings provide a novel perspective and lay the foundation for future research on the potential roles of lncRNAs, TFs, and mRNAs in TSCC. Verification of the potential lncRNA-TF-mRNA regulatory networks will provide a more comprehensive understanding of the pathogenesis of TSCC.

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“…Among them, long noncoding RNAs (lncRNAs) are RNA transcripts longer than 200 nucleotides and have no obvious protein-coding capacity. LncRNAs affect various aspects of biological processes in TSCC, including cell proliferation, tumorigenesis, survival, apoptosis, and migration [3] [4] [5] [6] . However, the underlying mechanisms have not been fully clarified.…”

Section: Introductionmentioning

confidence: 99%

“…To date, multiple lncRNAs have been identified in TSCC. For example, upregulation of the lncRNA FALEC suppresses malignant behaviors in TSCC [6] . Overexpression of the lncRNA HOTTIP in TSCC patients indicates a poor clinical prognosis [5] .…”

Section: Introductionmentioning

confidence: 99%

Identification of Potential Prognostic Biomarkers for Tongue Squamous Cell Carcinoma

Zhang

et al. 2020

Preprint

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Background: Tongue squamous cell carcinoma (TSCC) is one of the most common types of oral cancer and has a poor prognosis owing to a limited understanding of the pathogenesis mechanisms. The purpose of this study was to explore and identify potential biomarkers in TSCC by integrated bioinformatics analysis. Methods: The RNA sequencing data and clinical characteristics of TSCC patients were downloaded from The Cancer Genome Atlas (TCGA), and then differentially expressed RNAs (DERNAs), including differentially expressed long noncoding RNAs (DElncRNAs) and differentially expressed messenger RNAs (DEmRNAs), were identified in TSCC by bioinformatics analysis. Subsequently, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Hallmark pathway analyses were used to analyze the DERNAs. Using univariate and multivariate Cox regression analyses, four-lncRNA and two-mRNA signatures were developed and used for predicting survival in TSCC patients. We established a risk model to predict the overall survival (OS) of TSCC patients based on the DERNAs with Kaplan–Meier analysis and the log-rank p test. Furthermore, weighted gene coexpression network analysis (WGCNA) was performed in Cytoscape, and a protein-protein interaction (PPI) network was established in the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) database. Results: A total of 2,006 DEmRNAs and 1,001 DElncRNAs were found to be dysregulated in TSCC. A total of 417 DERNAs were used to construct the coexpression network, and the PPI network included 103 DEmRNAs. Univariate regression analysis of the DERNAs revealed 51 DElncRNAs and 90 DEmRNAs that were associated with OS in TSCC patients. Multivariate Cox regression analysis demonstrated that four of those lncRNAs (MGC32805, RP1-35C21.2, RP11-108K3.1, RP11-109M17.2) and two mRNAs (CA9, GTSF1L) had significant prognostic value, and their cumulative risk score indicated that these four-lncRNA and two-mRNA signatures independently predicted OS in TSCC patients. Conclusions: The current findings provide novel insights into the molecular mechanisms of TSCC and identify four lncRNAs and two mRNAs that are potential biomarkers that may be independent prognostic signatures for TSCC diagnosis and treatment.

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Long noncoding RNA FALEC inhibits proliferation and metastasis of tongue squamous cell carcinoma by epigenetically silencing ECM1 through EZH2

Cited by 31 publications

References 29 publications

Long non‑coding RNA PRNCR1 exerts oncogenic effects in�tongue squamous cell carcinoma in�vitro and in�vivo by sponging microRNA‑944 and thereby increasing HOXB5�expression

Long non‑coding RNA PRNCR1 exerts oncogenic effects in�tongue squamous cell carcinoma in�vitro and in�vivo by sponging microRNA‑944 and thereby increasing HOXB5�expression

Analysis of Differentially Expressed Long Non-coding RNAs and the Associated TF-mRNA Network in Tongue Squamous Cell Carcinoma

Identification of Potential Prognostic Biomarkers for Tongue Squamous Cell Carcinoma

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