Long noncoding RNA CRNDE functions as a diagnostic and prognostic biomarker in osteosarcoma, as well as promotes its progression via inhibition of miR‐335‐3p

Yu, Yali; Wang, Leiming; Zheng, Yan; Shi, Zhisong; Wang, Guanghui

doi:10.1002/jbt.22734

Cited by 18 publications

(15 citation statements)

References 29 publications

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“…[ 10,11,29 ] lncRNAs are not only the vital early diagnostic and prognostic markers but are also the important target of the target gene therapy for OS. [ 12,30 ] LINC01410, a newly discovered lncRNA, exerted a vital part in enhancing the development of colon cancer, GC, PTC, cervical cancer, cholangiocarcinoma, and neuroblastoma by regulating cell proliferation, invasion, and migration. [ 15–20 ] Our results indicated that the LINC01410 level markedly elevated within OS cells and tissues, which indicated that LINC01410 upregulation within OS was possibly associated with OS development.…”

Section: Discussionmentioning

confidence: 99%

“…[ 6–9 ] Numerous lncRNAs are found to participate in OS development such as ANRIL, APTR, CRNDE, HOTTIP, and LINC01410. [ 10–14 ] LINC01410, was reported to promote the tumorigenesis of colon cancer, gastric cancer (GC), papillary thyroid carcinoma (PTC), cervical cancer, cholangiocarcinoma, and neuroblastoma. [ 15–20 ] Our research group also demonstrated that LINC01410 functions as an oncogene in OS cells.…”

Section: Introductionmentioning

confidence: 99%

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Long noncoding RNA LINC01410 promotes tumorigenesis of osteosarcoma cells via miR‐497‐5p/HMGA2 axis

Gao

Zhang

et al. 2021

J Biochem & Molecular Tox

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LINC01410 is a tumor promoter that is upregulated in some cancer types, such as osteosarcoma (OS). Nonetheless, its role in OS and the underlying molecular mechanism have not been fully understood. Hence, we sought to elucidate it. We performed reverse‐transcription quantitative polymerase chain reaction for examining LINC01410, miR‐497‐5p and HMGA2 levels. Additionally, we carried out the cell counting kit‐8 and Transwell assays for detecting cell proliferation and invasion/migration. Bioinformatics predicted that there was a miR‐497‐5p binding site in LINC01410 or HMGA2; meanwhile, miR‐497‐5p was found to interact with HMGA2 and LINC01410 through dual‐luciferase reporter assay. LINC01410 and HMGA2 were high, and miR‐497‐5p showed low expression in OS tissues and cells. Cell function assay demonstrated that LINC01410 or HMGA2 knockdown or miR‐497‐5p overexpression obviously restrained OS proliferation, invasion, and migration. Oppositely, inhibiting miR‐497‐5p had the opposite effects. Functionally, miR‐497‐5p bound with LINC01410 3′‐untranslated region and HMGA2 was found to be the miR‐497‐5p target gene. Lastly, LINC01410 enhanced OS cell growth, invasion, and migration via decreasing miR‐497‐5p expression, whereas increasing that of HMGA2. We have demonstrated that LINC01410 promoted OS development partly by miR‐497‐5p/HMGA2 signal transduction pathway and this provides a reference for studying the mechanism of LINC01410 in OS.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA LINC01410 promotes tumorigenesis of osteosarcoma cells via miR‐497‐5p/HMGA2 axis

Gao

Zhang

et al. 2021

J Biochem & Molecular Tox

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“…lncRNA CRNDE as a regulate gene often plays important function in cancer cells proliferation and matastasis [16]. Previous research have reported that lncRNA CRNDE was dysregulated in bladder cancer [17], renal cell carcinoma[18], gastric cancer, breast cancer and osteosarcoma [19]. By analyzed CRC tissue specimens with microarray and RT-PCR assays, LD.…”

Section: Discussionmentioning

confidence: 99%

LncRNA CRNDE Promoted Colorectal Cancer Cells Reisitance To Paclitaxel Through Wnt/β-Catenin Signaling Pathway

Tang

et al. 2021

Preprint

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Background The lncRNA colorectal neoplasia differentially expressed (lncRNA CRNDE) is commonly over-expressed in different human cancers and involved in different biological functions. Paclitaxel(PTX) is a tricyclic diterpenoid compound which often used as a natural anticancer drugs in cancer treatments. Although there have many research reports about the mechanisms of LncRNA involved in PTX treatment, there are no any research about lncRNA CRNDE and PTX resistance in colorectal cancer. The purpose of this study is to investigate the mechanisims of LncRNA CRNDE involving PTX resistance in colorectal cancer. Results We constructed lncRNA CRNDE over-expression vector and transfected it into SW620 cell. CCK8, Transwell experiments proved that over-expression of lnc CRNDE increased SW620 cells proliferation and invasion, while the si-CRNDE group was significantly decreased. over-expression CRNDE can significantly up-regulate β-catenin, c-myc, APC and Axin2 expression and affect the expression of cyclinD1 and CDK4 after treated with PTX. Conclusion lncRNA CRNDE promotes CRCs proliferation, invasion and migration. Over-expression of LncRNA CRNDE enhanced the reisitance of CRC to PTX through inhibition of Wnt/ β-catenin signaling pathway.

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“…As a class of non-coding RNA, miRNAs are short endogenous RNAs that are 22-25 nucleotides in length (17,18). A large number of miRNAs have been reported to participate in OS progression, including miR-873, miR-9, miR-708-5p, miR-183 and miR-17-5p (19)(20)(21)(22)(23).…”

Section: Circ-lrp6 Contributes To Osteosarcoma Progression By Regulat...mentioning

confidence: 99%

circ‑LRP6 contributes to osteosarcoma progression by regulating the miR‑141‑3p/HDAC4/HMGB1 axis

Dong

Zheng

et al. 2022

Int J Oncol

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Circular RNA-lipoprotein receptor 6 (circ-LRP6) serves a role in promoting the tumorigenesis of retinoblastoma, esophageal squamous cell cancer and oral squamous cell carcinoma; however, whether circ-LRP6 demonstrates the same effect in osteosarcoma (OS) is yet to be fully elucidated.The present study aimed to analyze the expression, role and potential molecular mechanism of circ-LRP6 in OS. The expression levels of circ-LRP6, microRNA (miR)-141-3p, histone deacetylase 4 (HDAC4) and high mobility group protein 1 (HMGB1) were evaluated by reverse transcriptionquantitative PCR in OS tissues and cell lines. Cell Counting Kit-8, Transwell and Matrigel assays were conducted to evaluate cell proliferation, migration and invasion, respectively. Western blotting was also performed to determine HDAC4 and HMGB1 protein expression levels. Bioinformatics and dual-luciferase reporter assays were used to predict and analyze the interactions between circ-LRP6 and miR-141-3p, miR-141-3p and HDAC4, as well as between miR-141-3p and HMGB1. Additionally, RNA immunoprecipitation was performed to verify the association between circ-LRP6 and miR-141-3p. The results confirmed that circ-LRP6 was highly expressed in OS tissues and cell lines. In addition, circ-LRP6 negatively regulated the expression of miR-141-3p and, in turn, miR-141-3p negatively regulated HDAC4 and HMGB1 expression. Functional assays revealed that circ-LRP6 knockdown inhibited the proliferation, migration and invasion of OS cells, whereas the inhibition of miR-141-3p or the overexpression of either HDAC4 or HMGB1 partly reversed the inhibitory effect of circ-LRP6 knockdown. In summary, the present study determined that circ-LRP6 knockdown inhibited the proliferation, migration and invasion of OS cells by regulating the miR-141-3p/HDAC4/HMGB1 axis.

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Long noncoding RNA CRNDE functions as a diagnostic and prognostic biomarker in osteosarcoma, as well as promotes its progression via inhibition of miR‐335‐3p

Cited by 18 publications

References 29 publications

Long noncoding RNA LINC01410 promotes tumorigenesis of osteosarcoma cells via miR‐497‐5p/HMGA2 axis

Long noncoding RNA LINC01410 promotes tumorigenesis of osteosarcoma cells via miR‐497‐5p/HMGA2 axis

LncRNA CRNDE Promoted Colorectal Cancer Cells Reisitance To Paclitaxel Through Wnt/β-Catenin Signaling Pathway

circ‑LRP6 contributes to osteosarcoma progression by regulating the miR‑141‑3p/HDAC4/HMGB1 axis

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