Long noncoding RNA CCHE1 promotes cervical cancer cell proliferation via upregulating PCNA

Yang, Meng; Xu, Zeng‐Guang; Xia, Bairong; Wang, Yanying; Lou, Ge

doi:10.1007/s13277-015-3465-4

Cited by 91 publications

(98 citation statements)

References 35 publications

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“…lncRNAs constitute a heterogeneous group of RNA molecules in eukaryotic cells and play a role in a wide variety of biological processes (Hanahan and Weinberg 2000). Previous studies have shown that expression of several lncRNAs is dysregulated in cancer tissues, such as MEG3 (Luo et al 2015), CCHE1 (Yang et al 2015), and HOTAIR (Ding et al 2015). In the present study, high levels of Loc554202 were observed in cervical cancer tissues compared with the surrounding non-tumor cervical tissues.…”

Section: Discussionsupporting

confidence: 51%

Elevated Expression Levels of Long Non-Coding RNA, Loc554202, Are Predictive of Poor Prognosis in Cervical Cancer

Chen

Zhu

2017

Tohoku J. Exp. Med.

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Cervical cancer remains one of the most common causes of gynecological cancer-associated death. Long non-coding RNA Loc554202 (lncRNA Loc554202) has been reported to be involved in the development of several types of cancer. However, the role of Loc554202 in cervical cancer remains unclear. In this study, we measured the expression levels of Loc554202 in cervical cancer tissues from 120 patients. The quantitative real-time PCR analysis showed the expression levels of Loc554202 were significantly higher in cervical cancer tissues compared with the adjacent non-tumor tissues. Elevated expression levels of Loc554202 were significantly associated with tumor size (p = 0.006), FIGO stage (p = 0.015), HPV (p = 0.001), and lymph node metastasis (p = 0.002). Kaplan-Meier analysis clearly illustrated that patients with high expression levels of Loc554202 had a lower overall survival rate compared to patients with lower expression (p = 0.0013). Furthermore, we show that Loc554202 is an independent poor prognostic factor through multivariate analysis. Subsequently, using cervical cancer cell lines, HeLa and ME-180, we decreased the expression levels of Loc554202 with siRNA. As results, the proliferation ability of cervical cancer cells was inhibited and apoptosis was induced after Loc554202 knockdown, as judged by viability assay, colony formation, and flow cytometry. Moreover, knockdown of Loc554202 expression downregulated Bcl-2 expression and conversely up-regulated Bax expression in cervical cancer cells using Western blotting analysis. In conclusion, elevated levels of Loc554202 are predictive of poor prognosis in cervical cancer. We suggest that Loc554202 may serve as a potential therapeutic target for cervical cancer.

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Section: Discussionsupporting

confidence: 51%

Elevated Expression Levels of Long Non-Coding RNA, Loc554202, Are Predictive of Poor Prognosis in Cervical Cancer

Chen

Zhu

2017

Tohoku J. Exp. Med.

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“…However, many recent studies have examined the roles of various lncRNAs in cancer; examples include MALAT1 in small cell lung carcinoma [38, 39], lncRNA-ATB in hepatocellular carcinoma, and NKILA in breast cancer [40, 41]. Many lncRNAs also participate in the progression of cervical cancer, affecting proliferation, apoptosis, metastasis, radio-resistance, and HPV [42–44]. Many potentially cancer-relevant lncRNAs have not yet been characterized, and future lncRNA studies may help greatly improve individualized diagnosis techniques [45, 46].…”

Section: Discussionmentioning

confidence: 99%

Lnc-CC3 increases metastasis in cervical cancer by increasing Slug expression

et al. 2016

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Although screening has reduced mortality rates, metastasis still results in poor survival and prognosis in cervical cancer patients. We compared cervical cancer ESTs libraries with other ESTs libraries to identify candidate genes and cloned a novel cervical cancer-associated lncRNA, lnc-CC3. Overexpression of lnc-CC3 promoted migration and invasion by SiHa cervical cancer cells in vitro and in vivo, increased Slug expression, and reduced the expression of the epithelial cell marker E-cadherin. Conversely, lnc-CC3 knockdown altered SiHa cell morphology and increased the expression of E-cadherin, thereby suppressing migration and invasion. These results suggest lnc-CC3 may be a useful marker of metastasis in cervical cancer.

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“…LncRNAs may exert their biological functions via regulating expression of cancer-associated genes in transcriptional level or post-transcriptional level [33][34][35][36]. However, LET has been reported to bind and downregulate nuclear factor 90 protein abundance in hepatocellular carcinoma [21].…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA-LET, which is repressed by EZH2, inhibits cell proliferation and induces apoptosis of nasopharyngeal carcinoma cell

Sun

Liu

et al. 2015

Med Oncol

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Recent studies have reported that long noncoding RNAs (lncRNAs) play critical roles in carcinogenesis and progression. LncRNA-LET, a recently identified lncRNA, has been shown to be a tumor suppressor in hepatocellular carcinoma. However, the expression and functional of lncRNA-LET in other type of cancers remain largely unknown. In this study, we found that lncRNA-LET was significantly downregulated in nasopharyngeal carcinoma (NPC) tissues compared with corresponding normal tissues. Decreased LET expression is significantly correlated with advanced clinical stage, larger tumor size, increased lymph node tumor burden, and poor survival of NPC patients. Gain- and loss-of-function experiments demonstrated that enhanced LET expression inhibited NPC cells proliferation and induced cell apoptosis. By contrast, the knockdown of LET promoted NPC cells proliferation and inhibited cell apoptosis. Importantly, we found lncRNA-LET is transcriptional repressed by EZH2-mediated H3K27 histone methylation on the LET promoter. The expressions of EZH2 and lncRNA-LET are significantly inversely correlated in NPC tissues. Collectively, these findings indicate a pivotal role for lncRNA-LET in NPC cell proliferation and apoptosis, and reveal an epigenetic mechanism for lncRNA-LET dysregulation.

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Long noncoding RNA CCHE1 promotes cervical cancer cell proliferation via upregulating PCNA

Cited by 91 publications

References 35 publications

Elevated Expression Levels of Long Non-Coding RNA, Loc554202, Are Predictive of Poor Prognosis in Cervical Cancer

Elevated Expression Levels of Long Non-Coding RNA, Loc554202, Are Predictive of Poor Prognosis in Cervical Cancer

Lnc-CC3 increases metastasis in cervical cancer by increasing Slug expression

Long noncoding RNA-LET, which is repressed by EZH2, inhibits cell proliferation and induces apoptosis of nasopharyngeal carcinoma cell

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