Long noncoding RNA CASC9 promotes LIN7A expression via miR‐758‐3p to facilitate the malignancy of ovarian cancer

Hu, Xiaowei; Li, Yang; Kong, Dan; Hu, Longhu; Liu, Donghui; Wu, Jin

doi:10.1002/jcp.27903

Cited by 54 publications

(50 citation statements)

References 24 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As the results show, MAGI1-IT1 was principally localized in the cytoplasm, with weak expression in the nucleus (Figure 4(a)). Since cytoplasmic lncRNAs have the ability to bind to many biomolecules, ceRNAs have emerged as an important mechanism for lncRNAs and miRNAs regulatory networks [26,27]. Thus, the associations among MAGI1-IT 1, corresponding miRNAs and mRNAs were subsequently predicted.…”

Section: Magi1-it1 May Serve As a Cerna Of Mir-200a In Regulating Zebmentioning

confidence: 99%

RETRACTED ARTICLE: Long noncoding RNA MAGI1-IT1 promoted invasion and metastasis of epithelial ovarian cancer via the miR-200a/ZEB axis

Gao

Zhan

et al. 2019

Cell Cycle

View full text Add to dashboard Cite

Epithelial ovarian cancer (EOC) is the most lethal gynecologic malignancy, and its vulnerability to metastasis contributes to the poor outcomes of EOC patients. Long noncoding RNAs (lncRNAs) were verified to play a pivotal role in EOC metastasis. However, the potential role of lncRNA membraneassociated guanylate kinase inverted 1 (MAGI1) intronic transcript (MAGI1-IT1) in EOC is largely unknown. In this study, the function and mechanisms of MAGI1-IT1 in EOC metastasis were explored profoundly. First, MAGI1-IT1 expression was found to be significantly decreased in overexpressing miR-200a EOC cells. Second, MAGI1-IT1 expression was remarkably increased in metastatic EOC tissues, and high MAGI1-IT1 was dramatically associated with EOC FIGO III-IV stage; in addition, MAGI1-IT1 might be related to EOC dissemination via epithelial-mesenchymal transition (EMT). Next, a series of gain-and loss-of-function assays verified that, although MAGI1-IT1 has no significant role in EOC proliferation and subcutaneous xenograft growth, the upregulation of MAGI1-IT1 can remarkably facilitate EOC EMT phenotype, cells migration and invasion ability and intraperitoneal metastasis in nude mice, while downregulation of MAGI1-IT1 led to the opposite effect in vitro. Moreover, MAGI1-IT1 was validated to promote EOC metastasis through upregulation of ZEB1 and ZEB2 by competitively binding miR-200a, and the restrictive effects of MAGI1-IT1 depletion on EOC metastasis could be reversed by inhibition of miR-200a and upregulation of ZEB1 and ZEB2. Collectively, these results suggest that MAGI1-IT1 may work as a ceRNA in promoting EOC metastasis through miR-200a and ZEB1/2 and may be a potential therapeutic target for EOC.

show abstract

Section: Magi1-it1 May Serve As a Cerna Of Mir-200a In Regulating Zebmentioning

confidence: 99%

RETRACTED ARTICLE: Long noncoding RNA MAGI1-IT1 promoted invasion and metastasis of epithelial ovarian cancer via the miR-200a/ZEB axis

Gao

Zhan

et al. 2019

Cell Cycle

View full text Add to dashboard Cite

show abstract

“…Moreover, CASC9 has been identified as a tumor promoter in multiple cancers. For instance, CASC9 promotes the development of ovarian cancer by regulating miR-758-3p/LIN7A axis [15]. Attenuation of CASC9 accelerates GC cell apoptosis [16].…”

Section: Introductionmentioning

confidence: 99%

CASC9 Facilitates Cell Proliferation in Bladder Cancer by Regulating CBX2 Expression

Huo

Tan

Chen

2020

Nephron

View full text Add to dashboard Cite

Background: As the seventh most common urologic carcinoma worldwide, approximately 430,000 patients are diagnosed with bladder cancer (BC) every year. Increasing evidence indicates that long noncoding RNAs (lncRNAs) play crucial roles in the progression of BC. Objectives: This study is aimed to explore the function and mechanism of CASC9 in BC. Methods: Bioinformatics analysis and experiments including RT-qPCR, luciferase reporter, Cell Counting Kit-8 assay, Western blot, RNA immunoprecipitation assay, and TU-NEL staining were applied to explore the function and mechanism of CASC9 in BC tissues and cell lines. Results: Our study demonstrated that CASC9 was upregulated in BC tissues and cell lines. Moreover, we found that CASC9 knockdown notably decreased proliferation while increased apoptotic rate in BC cells. Mechanistically, bioinformatics prediction and following experiments indicated that CASC9 worked as a competing endogenous RNA (ceRNA) of CBX2 through sponging miR-497-5p. Meanwhile, we recognized that CASC9 and miR-497-5p negatively regulated each other in a mutual way. Furthermore, we found that miR-497-5p shared binding site with CBX2. In addition, miR-497-5p could negatively regulated CBX2, while CASC9 could positively regulated CBX2. Rescue assays reveled that CBX2 overexpression could reversed the reduction of cell proliferation or the enhancement of cell apoptosis induced by CASC9 suppression. Conclusions: Our study manifests the first evidence that CASC9 serves as an oncogene in BC and accelerates cell proliferation by modulating miR-497-5p/CBX2 axis. The present study may provide a cogitable target for BC therapy.

show abstract

“…These results imply that miR-758 serves as a tumor suppressor in CRC progression. Previous studies have also reported the downregulation of miR-758 in human cancers, such as ovarian and gastric cancer (17,18). Also, miR-758 has been found to inhibit cell migration, invasion and proliferation in bladder cancer, hepatocellular carcinoma and glioblastoma (19)(20)(21).…”

Section: Discussionmentioning

confidence: 92%

MicroRNA‑758 acts as a tumor inhibitor in colorectal cancer through targeting PAX6 and regulating PI3K/AKT pathway

Zhang¹,

Zhang

et al. 2020

Oncol Lett

View full text Add to dashboard Cite

Recently, a number of microRNAs (miRNAs) have been reported to play different roles in human cancers, including colorectal cancer (CRC). However, the specific role of miR-758 has not been clarified in CRC. Therefore, the aim of the present study was to explore the role of miR-758 in CRC. RT-qPCR and western blot analysis were used to quantify the expression of miR-758 and genes. The function of miR-758 in CRC was investigated using Transwell, CCK-8 and luciferase reporter assays. According to the results, the downregulation of miR-758 expression was associated with aggressive behavior and poor prognosis in CRC patients. miR-758 was shown to restrain the cell viability and metastasis in CRC. In addition, it was confirmed that miR-758 directly targets PAX6 and inhibits CRC progression through targeting PAX6. The results also revealed that miR-758 blocked EMT and PI3K/AKT pathway in CRC. In conclusion, miR-758 acts as a tumor suppressor in CRC by downregulating PAX6.

show abstract

Long noncoding RNA CASC9 promotes LIN7A expression via miR‐758‐3p to facilitate the malignancy of ovarian cancer

Cited by 54 publications

References 24 publications

RETRACTED ARTICLE: Long noncoding RNA MAGI1-IT1 promoted invasion and metastasis of epithelial ovarian cancer via the miR-200a/ZEB axis

RETRACTED ARTICLE: Long noncoding RNA MAGI1-IT1 promoted invasion and metastasis of epithelial ovarian cancer via the miR-200a/ZEB axis

CASC9 Facilitates Cell Proliferation in Bladder Cancer by Regulating CBX2 Expression

MicroRNA‑758 acts as a tumor inhibitor in colorectal cancer through targeting PAX6 and regulating PI3K/AKT pathway

Contact Info

Product

Resources

About