Long noncoding RNA CASC7 inhibits the proliferation and migration of papillary thyroid cancer cells by inhibiting miR-34a-5p

Sun, Wencong; Yin, Dengke

doi:10.1186/s12576-021-00793-2

Cited by 10 publications

(6 citation statements)

References 24 publications

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“…Prior research demonstrated that lncRNAs might operate as a miRNA sponge, modulating the miRNA expression and competing for miRNA (22). By targeting miR-34a-5p, CASC7 restricts the proliferation and migration of papillary thyroid cancer cells (23), and by downregulating miR-10a, CASC7 may upregulate phosphatase and tensin homolog to restrict neuroblastoma cell growth (24). CASC7 prevents colon cancer cell growth and migration via restricting miRNA-21 (20).…”

Section: Discussionmentioning

confidence: 99%

LncRNA CASC7 acts as a tumor suppressor in bladder cancer through modulating miR-103a-5p/TFPI2 axis.

XIAO

Ning

Ruan

2022

Preprint

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Background It was demonstrated that aberrant expression of long noncoding RNAs (LncRNAs) exerts regulatory effects on several malignancies. The lncRNA CASC7 effect on bladder cancer (BC) was not demonstrated. CASC7 role in BC was investigated in the current study. Material and Methods CASC7 expression was investigated. Using a luciferase reporter test, the link between CASC7, miR-103a-5p, and TFPI2 was confirmed. CCK-8 and flow cytometry assay were utilized for detecting apoptosis and cell viability. Transwell and wound healing tests were utilized for evaluating the invasion and migration cell capabilities. For analyzing the TFPI2, p-GSK-3β, β-catenin, and GSK-3β protein level, western blot was utilized. Results CASC7 expression greatly decreased in BC cell lines and clinical specimens. In function loss and gain assays, CASC7 prevents migration, proliferation, and invasion of the cell and induces cell death in BC cells, in accordance with research. In addition, CASC7 has been reported to enhance TFPI2 expression and negatively regulate the miR-103a-5p expression. Consequently, TFPI2 as a downstream target of miR-103a-5p and miR-103a-5p as a direct target of CASC7 were investigated. Moreover, over-expression of CASC7 markedly reduced the p-GSK-3β, β-catenin, expression. In contrast, the knockdown of CASC7 had the opposite effect. Conclusion Our findings showed that BC CASC7 functions as a tumor suppressor via miR-103a-5p and TFPI2. Its regulatory impact is related to the miR-103a-5p/TFPI2/Wnt/ β-catenin signaling pathway. In the BC treatment, CASC7 can serve as a biomarker or therapeutic target.

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Section: Discussionmentioning

confidence: 99%

LncRNA CASC7 acts as a tumor suppressor in bladder cancer through modulating miR-103a-5p/TFPI2 axis.

XIAO

Ning

Ruan

2022

Preprint

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“…Recently, several studies have revealed the role of CASC7 in other cancers and pathologies. It was reported that CASC7 retarded the progression of multiple cancers, such as papillary thyroid carcinoma, neuroblastoma, glioma and non-small-cell lung cancer [ 9 , 10 , 26 , 27 ]. All these researches uniformly pointed out that lncRNA CASC7 is a cancer suppressor.…”

Section: Discussionmentioning

confidence: 99%

“…Among the many lncRNAs, lncRNA CASC7 (cancer susceptibility candidate 7) is an RNA gene and is affiliated with the lncRNA class [ 8 ]. It has been previously reported that lncRNA CASC7 functions as a tumor suppressor in both non-small-cell lung cancer and thyroid cancer [ 9 , 10 ]. Besides, lncRNA CASC7 is associated with several pathologies, such as heart failure and severe asthma [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA CASC7 suppresses malignant behaviors of breast cancer by regulating miR-21-5p/FASLG axis

et al. 2021

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Recently, it has been increasingly proved that lncRNAs are functionally involved in a majority of tumor progression. LncRNA CASC7 has also been revealed to participate in the development of several cancers as a tumor promoter or suppressor. Herein, we focus on uncovering the role and underlying molecular mechanism of CASC7 in breast cancer. Tumor tissues and the paired paracancerous tissues from the breast cancer patients were used to evaluate the level of CASC7 in breast cancer. By analyzing the CASC7 expression in breast cancer cell lines, both the expression levels of CASC7 in cancer tissues and cell lines were obviously downregulated compared to those in paired paracancerous tissues and normal human epithelial MCF10A cells. Subsequently, the construction of lentivirus overexpression system (oe-CASC7 and oe-NC) was used to elevate the expression of CASC7. A series of functional experiments were conducted to show that the cell proliferation, migration, and invasion were inhibited when CASC7 overexpressed in breast cancer cells. Meanwhile, the apoptosis of oe-CASC7 cells was induced compared to the oe-NC breast cancer cells. We further confirmed that CASC7 functions by regulating miR-21-5p/FASLG axis. Finally, a xenograft model in nude mice verified that CASC7 was a tumor suppressor in breast cancer. These results suggest that lncRNA CASC7 suppresses to malignant behaviors of breast cancer by modulating miR-21-5p/FASLG axis. Abbreviations lncRNAs: long non-coding RNAs; ceRNA: competing endogenous RNA; CASC7: cancer susceptibility candidate 7; miRNAs: MicroRNAs; MAPK10: mitogen-activated protein kinase 10; FASLG: Tumor Necrosis Factor Ligand Superfamily Member 6; FAS: Tumor Necrosis Factor Receptor Superfamily Member 6

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“… Diagnosis 4 cell lines China 2019 Lu [ 119 ] miR-34a-5p TP73 CASC7 suppressed the PTC cell proliferation and migration by miR-34a-5p sponging and TP73 up regulation. Diagnosis 30 patients 2 cell lines China 2021 Sun [ 120 ] miR-214 PSMD10 MiR-214 suppressed PTC cell migration and EMT via PSMD10 targeting. Diagnosis and prognosis 30 patients 2 cell lines China 2018 Liu [ 121 ] miR-4319 SMURF1 MiR-4319 repressed the TC cell migration and EMT through SMURF1 targeting.…”

Section: Introductionmentioning

confidence: 99%

“…TP73 belongs to the p53 protein family that is involved in cell cycle and apoptosis regulation. CASC7 suppressed the PTC cell proliferation and migration by miR-34a-5p sponging and TP73 up regulation [ 120 ].…”

Section: Introductionmentioning

confidence: 99%

MicroRNAs as the critical regulators of cell migration and invasion in thyroid cancer

et al. 2022

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Thyroid cancer (TC) is one of the most frequent endocrine malignancies that is more common among females. Tumor recurrence is one of the most important clinical manifestations in differentiated TC which is associated with different factors including age, tumor size, and histological features. Various molecular processes such as genetic or epigenetic modifications and non-coding RNAs are also involved in TC progression and metastasis. The epithelial-to-mesenchymal transition (EMT) is an important biological process during tumor invasion and migration that affects the initiation and transformation of early-stage tumors into invasive malignancies. A combination of transcription factors, growth factors, signaling pathways, and epigenetic regulations affect the thyroid cell migration and EMT process. MicroRNAs (miRNAs) are important molecular factors involved in tumor metastasis by regulation of EMT-activating signaling pathways. Various miRNAs are involved in the signaling pathways associated with TC metastasis which can be used as diagnostic and therapeutic biomarkers. Since, the miRNAs are sensitive, specific, and non-invasive, they can be suggested as efficient and optimal biomarkers of tumor invasion and metastasis. In the present review, we have summarized all of the miRNAs which have been significantly involved in thyroid tumor cells migration and invasion. We also categorized all of the reported miRNAs based on their cellular processes to clarify the molecular role of miRNAs during thyroid tumor cell migration and invasion. This review paves the way of introducing a non-invasive diagnostic and prognostic panel of miRNAs in aggressive and metastatic TC patients.

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Long noncoding RNA CASC7 inhibits the proliferation and migration of papillary thyroid cancer cells by inhibiting miR-34a-5p

Cited by 10 publications

References 24 publications

LncRNA CASC7 acts as a tumor suppressor in bladder cancer through modulating miR-103a-5p/TFPI2 axis.

LncRNA CASC7 acts as a tumor suppressor in bladder cancer through modulating miR-103a-5p/TFPI2 axis.

Long non-coding RNA CASC7 suppresses malignant behaviors of breast cancer by regulating miR-21-5p/FASLG axis

MicroRNAs as the critical regulators of cell migration and invasion in thyroid cancer

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