Long non‐coding <scp>RNA NEAT</scp>1 promoted ovarian cancer cells’ metastasis through regulation of miR‐382‐3p/<scp>ROCK</scp>1 axial

Liu, Yangcheng; Wang, Yong; Xiao, Fu; Lu, Zhi

doi:10.1111/cas.13647

Cited by 76 publications

(55 citation statements)

References 46 publications

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“…Emerging studies have validated that numerous lncRNA are aberrantly expressed in EOC and play crucial roles in tumorigenesis and progression . For instance, the expression of the lncRNA human ovarian cancer‐specific transcript 2 (HOST2) was found to be upregulated in EOC tissue, promoting cell proliferation and metastasis by sponging miRNA let‐7b .…”

Section: Discussionmentioning

confidence: 99%

“…Emerging studies have validated that numerous lncRNA are aberrantly expressed in EOC and play crucial roles in tumorigenesis and progression. 15,16 For instance, the expression of the lncRNA human ovarian cancer-specific transcript 2 (HOST2) was found to be upregulated in EOC tissue, promoting cell proliferation and metastasis by sponging miRNA let-7b. 17 In addition, highly expressed homeobox (HOX) transcript antisense intergenic RNA (HOTAIR) was found to be closely related to poor EOC prognosis and the promotion of EOC migration and invasion through matrix metalloproteinases (MMP) and epithelial-mesenchymal transition (EMT) in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 99%

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LncRNA TPT1‐AS1 promotes tumorigenesis and metastasis in epithelial ovarian cancer by inducing TPT1 expression

Wei

Gao

et al. 2019

Cancer Science

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Increasing numbers of studies have confirmed that long noncoding RNA (lnc RNA ) play a critical role in epithelial ovarian cancer ( EOC ) progression. However, the potential function of the lnc RNA tumor protein translationally controlled 1 ( TPT 1) antisense RNA 1 ( TPT 1‐ AS 1) in EOC is unclear. In this study, we aimed to uncover the biological roles and regulatory mechanisms of TPT 1‐ AS 1 in EOC progression and metastasis. First, TPT 1‐ AS 1 expression was significantly higher in EOC metastatic tissue and cell lines than in their respective control counterparts. In addition, ectopic TPT 1‐ AS 1 expression was strongly associated with unfavorable EOC clinicopathological features, including FIGO stage, tumor size and tumor differentiation. TPT 1‐ AS 1 overexpression remarkably induced cell proliferation, migration and invasion, and significantly attenuated cell adhesion ability in vitro and facilitated nude mouse subcutaneous xenograft growth and intraperitoneal metastasis in vivo, while the downregulation of TPT 1‐ AS 1 expression produced the opposite effect in vitro. Mechanistically, TPT 1‐ AS 1 was proven to be primarily distributed in EOC cell nuclei and positively modulated TPT 1 promoter activity and transcription. Moreover, the oncogenic effects of TPT 1‐ AS 1 could be reversed by TPT 1 depletion, and the PI 3K/ AKT signaling pathway downstream of TPT 1 was also altered. These results suggested that TPT 1‐ AS 1 induced EOC tumor growth and metastasis through TPT 1 and downstream PI 3K/ AKT signaling and that TPT 1‐ AS 1 may be a promising therapeutic target for EOC .

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

LncRNA TPT1‐AS1 promotes tumorigenesis and metastasis in epithelial ovarian cancer by inducing TPT1 expression

Wei

Gao

et al. 2019

Cancer Science

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“…Several researches have been said that ROCK1/F-actin pathways contributed to the mitochondrial related cell apoptosis, migration inhibition, and proliferation arrest in several cancer cells. 36,37 Thus, a question was raised whether miR-31 functions in HepG2 cells through ROCK1/F-actin pathways. To answer it, Y-27632, the inhibitor of ROCK1, was used in HepG2 cells transfected with miR-31 mimic.…”

Section: Mir-31 Activates Hepg2 Cell Injury Via Rock1/f-actin Pathwaysmentioning

confidence: 99%

<p>miR-31 Modulates Liver Cancer HepG2 Cell Apoptosis and Invasion via ROCK1/F-Actin Pathways</p>

Zhang¹,

Xu²,

Yang³

2020

OTT

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Purpose: Liver cancer is one of the most common malignant tumor in the world. miR-31 is downregulated in liver cancer and associated with tumor growth and metastasis. However, the underlying mechanism remains unclear. Methods: Cellular apoptosis was detected via MTT, TUNEL assay, LDH release and Annexin V/PI flow-cytometry analysis. Cellular migration and invasion were measured by the Transwell chamber assay. Mitochondrial functions were evaluated via mitochondrial membrane potential JC-1 staining and mPTP opening assessment. The mitophagy activity was examined via Western blots. Results: In the present study, our results confirm that miR-31 promotes apoptosis and inhibits proliferation and metastasis in liver cancer HepG2 cells. In vitro, miR-31 promotes HepG2 cell apoptosis through the mitochondrial pathway as indicated by mitochondrial potential reduction, increased mPTP opening time, cty-c release and imbalance of pro-and anti-apoptotic proteins. Furthermore, miR-31 reduces the energy generation by inhibiting mitochondrial respiratory function. At last, it is demonstrated that miR-31 triggers the mitochondrial damage via ROCK1/F-actin pathway. Inhibiting the ROCK1/F-actin pathway abolishes the effects of miR-31 mimic on mitochondrial injury, apoptosis, proliferation arrest and migration inhibition. Conclusion: Our results reveal that miR-31 can inhibit HepG2 cell survival and metastasis by activating the ROCK1/F-actin pathway.

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“…The nuclear paraspeckle assembly transcript 1 (NEAT1) was a newly identified lncRNA that regulated gene expression by retaining mRNAs for editing in the nucleus (Clemson et al, ). It was reported as an oncogene in several types of human cancers, including hepatocellular carcinoma, lung cancer, oesophageal cancer, colorectal cancer, renal cell carcinoma, ovarian cancer, breast cancer, and glioma (F. Liu et al, ; Y. Liu, Wang, Fu, & Lu, ; Yu, Li, Zheng, Chan, & Wu, ; Zhou et al, ). However, the role of NEAT1 in RB remains unknown.…”

Section: Introductionmentioning

confidence: 99%

“…Studies have shown that NEAT1 was elevated in multiple cancers, and it acted as an oncogene in these cancers (F. Liu et al, 2017;Y. Liu et al, 2018;Yu et al, 2017).…”

mentioning

confidence: 99%

Long noncoding RNA NEAT1 promotes the growth of human retinoblastoma cells via regulation of miR‐204/CXCR4 axis

Zhong

Yang

et al. 2018

Journal Cellular Physiology

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Retinoblastoma (RB) is an aggressive eye cancer of infancy and childhood with high mortality. Studies have shown that long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) is closely related to the progression of multiple cancers. However, its role in RB remains unknown. This study aimed to investigate the role and underlying mechanism of NEAT1 in RB. We first detected the expression of NEAT1 in human RB tissues and cell lines. The effects of NEAT1 on the proliferation, migration, and apoptosis of RB cells were analyzed by loss‐of‐function. The underlying mechanism of NEAT1 in RB was mainly focused on the microRNA 204/C‐X‐C chemokine receptor type 4 (miR‐204/CXCR4) axis. In addition, the role and mechanism of NEAT1 in RB were further evaluated in a mouse xenograft tumor model. We found NEAT1 and CXCR4 expression levels were elevated, whereas miR‐204 expression was decreased in RB tissues and cells. Downregulation of NEAT1 significantly decreased the proliferation and migration but promoted the apoptosis of RB cells. NEAT1 functioned as a competing endogenous RNA for miR‐204 to regulate CXCR4 expression. Knockdown of NEAT1 suppressed the tumor volume, tumor weight, and CXCR4 expression, whereas increased miR‐204 expression in mice. In conclusion, NEAT1 promotes the development of RB via miR‐204/CXCR4 axis, which provides a new target for the treatment of RB disease.

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Long non‐coding RNA NEAT1 promoted ovarian cancer cells’ metastasis through regulation of miR‐382‐3p/ROCK1 axial

Cited by 76 publications

References 46 publications

LncRNA TPT1‐AS1 promotes tumorigenesis and metastasis in epithelial ovarian cancer by inducing TPT1 expression

LncRNA TPT1‐AS1 promotes tumorigenesis and metastasis in epithelial ovarian cancer by inducing TPT1 expression

<p>miR-31 Modulates Liver Cancer HepG2 Cell Apoptosis and Invasion via ROCK1/F-Actin Pathways</p>

Long noncoding RNA NEAT1 promotes the growth of human retinoblastoma cells via regulation of miR‐204/CXCR4 axis

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