Long non‐coding <scp>RNA FAL</scp>1 functions as a ce<scp>RNA</scp> to antagonize the effect of miR‐637 on the down‐regulation of <scp>AKT</scp>1 in Hirschsprung's disease

Yang, Li; Zhou, Lingling; Lu, Changgui; Shen, Qiyang; Su, Yang; Zhi, Zhengke; Wu, Feng; Zhang, Hua; Wen, Zechao; Chen, Guanglin; Li, Hongxing; Xia, Yankai; Tang, Weibing

doi:10.1111/cpr.12489

Cited by 51 publications

(41 citation statements)

References 33 publications

(67 reference statements)

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“…CDK6, STAT3, AKT1) ( Table 2). In addition, miR-637 could be itself regulated by non-coding endogenous competitors other than solely circHIPK3, as described in other diseases (26)(27)(28)(29)(30)(31)(32)(33)(34) (Table 2).…”

Section: A Comprehensive Analysis Of Circ-0000284 (Circhipk3) In Ccamentioning

confidence: 93%

Exosomal circRNAs: new players in the field of cholangiocarcinoma

2019

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Cholangiocarcinoma (CCA) is a deadly cancer worldwide associated with limited therapeutic options. A recent study published in Clinical Science by Wang and colleagues [Clin. Sci. (2019) 133(18), 1935–1953] brought new perspectives to CCA management and therapy by focusing on circular RNAs (circRNAs). CircRNAs belong to an emerging class of functional non-coding RNAs (ncRNAs) regulating numerous biological processes. Notably, circRNAs have been associated with cancer onset and progression, although reports in CCA are very limited so far. In this work, the expression of circular RNA circ-0000284 (aka circHIPK3) was specifically elevated in CCA cell lines, human tumor tissues and plasma exosomes. Gain and loss of function approaches were performed to better understand the molecular mechanisms regulated by circ-0000284. Notably, the authors evaluated the role of circ-0000284 as a microRNA (miRNA) sponge. By prediction analysis and functional tests, a direct interaction was demonstrated with miR-637 that targets lymphocyte antigen-6 E (LY6E). Increased expression of circ-0000284 was associated with enhanced migration, invasion and proliferation of CCA cell lines. Interestingly, exosomal-mediated circ-0000284 was reported to exhibit pro-oncogenic effects on surrounding normal cells. Altogether, these data highlight circRNAs not only as new players in CCA pathogenesis but also as promising molecules for innovative non-invasive biomarkers, as circRNAs are enriched and stable in exosomes. Further investigations on extracellular vesicles should provide the necessary tools to improve CCA diagnosis, and move toward targeted-therapies.

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Section: A Comprehensive Analysis Of Circ-0000284 (Circhipk3) In Ccamentioning

confidence: 93%

Exosomal circRNAs: new players in the field of cholangiocarcinoma

2019

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“…To reveal how lncRNAs mediate transcription in colon adenocarcinoma through their influence on mRNA and miRNA binding, a ceRNA network based on the list of DElncRNAs, DEmiRNAs, and DEmRNAs were constructed and visualized using Cytoscape software. As shown in Figure S2, the lncRNA-miRNA-mRNA network was comprised of 135 lncRNA, 28 miRNA and 52 mRNA nodes (Table S4- 5). The majority of DEmRNAs were tumor-related genes including SERPINE1, NPTX1, HILPDA, PRSS22, PMAIP1.…”

Section: Construction Of the Cerna Networkmentioning

confidence: 99%

“…LncRNAs are ≥200 nucleotides in length and regulate an array of cell biological processes including chromatin remodeling, transcription, and post-transcriptional events. 4,5 The most recognized molecular mechanism of lncRNAs are to act as miRNA "sponges" to regulate downstream target genes. 6,7 LncRNAs act as competing endogenous RNAs (ceRNAs) to accelerate cancer proliferation, migration, and invasion.…”

Section: Introductionmentioning

confidence: 99%

LINC00491 as a new molecular marker can promote the proliferation, migration and invasion of colon adenocarcinoma cells

Wan

Deng

Wang

et al. 2019

OTT

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Background: Long non-coding RNAs (lncRNAs) play an important role in the pathogenesis of multiple tumors. However, the roles of lncRNAs during colon adenocarcinoma and cancer progression remain unclear. This study aimed identify new lncRNAs that act as molecular markers for the prevention and diagnosis of colon adenocarcinoma. Methods: RNA sequencing (RNA-Seq) data associated with colon adenocarcinoma were retrieved from the Cancer Genome Atlas (TCGA). Biological processes in Gene Ontology (Go) and the Kyoto Encyclopedia of Genomes (KEGG) were searched for pathways at the significance level. The expression of LINC00491 and its downstream targets were assessed by real-time PCR, Western blotting and dual-luciferase assays. Biological functions of LINC00491 during cell proliferation, migration and invasion were assessed using CCK-8, colony formation assays, wound healing, and transwell invasion assays in colon adenocarcinoma HT-29 and HCT116 cells. Results: Bioinformatics analysis with the TCGA colon adenocarcinoma dataset showed that LINC00491 was significantly up-regulated in colon adenocarcinoma. Furthermore, we found that LINC00491 positively regulates SERPINE1 expression through sponging miR-145 and promoting the proliferation, migration, and invasion of colon adenocarcinoma cells, thus playing an oncogenic role during colon adenocarcinoma pathogenesis. Conclusion: LINC00491 functions as a ceRNA to promote SERPINE1 expression by sponging miR-145. LINC00491 serves as a therapeutic target and prognostic biomarker in colon adenocarcinoma.

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“…9 Up to now, many miRNAs such as miR- 150-5p, miR-143, miR-637, miR-483-3p, miR-1324, miR-215, miR-24-1, let-7a, miR-939, miR-770-5p, miR-369-3p, miR-483-5p, miR-206 and miR-214 have been shown to affect the occurrence and development of HSCR. [10][11][12][13][14][15][16][17][18][19][20][21] However, no research has explored the role of miR-100 to HSCR.…”

Section: Introductionmentioning

confidence: 99%

“…[27][28][29][30][31] Recently, some researches indicated that miR-100 regulated the growth, differentiation and apoptosis of neurons and directly/indirectly affected HSCR-associated pathways. 8,10,12,16,[32][33][34] Considering that rs1834306 may be a potential function site of miR-100, we conducted a case-control study to explore the association between miR-100 rs1834306 A>G polymorphism and HSCR susceptibility in Southern Chinese population.…”

Section: Introductionmentioning

confidence: 99%

miR-100 rs1834306 A>G Increases the Risk of Hirschsprung Disease in Southern Chinese Children

Zhu¹,

Ao²,

Zheng³

et al. 2020

PGPM

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Background: Hirschsprung disease (HSCR) is a rare congenital gastrointestinal disease characterized by the absence of intestinal submucosal and myometrial ganglion cells. Recently, researches indicated that miR-100 regulated the growth, differentiation and apoptosis of neurons, and affected the functions of HSCR-associated pathways. While miR-100 rs1834306 A>G polymorphism was shown to modify the susceptibility to tumors, the association between this polymorphism and HSCR susceptibility is still unknown. Methods: This was a case-control study consisting of 1470 HSCR cases and 1473 controls from southern China. DNA was genotyped by TaqMan real-time PCR. Odds ratios (ORs) and 95% confidence intervals (CIs) were used as statistical indicators. Results: We found that miR-100 rs1834306 G allele and GG genotype significantly increased HSCR susceptibility (GG vs AA: adjusted OR=1.31, 95% CI=1.04-1.64, P=0.020; G vs A: adjusted OR=1.12, 95% CI=1.01-1.25, P=0.041; GG vs AA/AG: adjusted OR=1.30, 95% CI=1.07-1.59, P=0.010). In the stratified analysis, miR-100 rs1834306 GG genotype carriers had higher risk to develop HSCR in all clinical subtypes when compared with those with AA/AG genotypes, and OR was rising with HSCR aggravation (SHSCR: adjusted OR=1.28, 95% CI=1.03-1.59, P=0.029; LHSCR: adjusted OR=1.48, 95% CI=1.06-2.07, P=0.020; TCA: adjusted OR=2.12, 95% CI=1.22-3.69, P=0.008). Conclusion: Our findings suggested that miR-100 rs1834306 A>G polymorphism was associated with increased HSCR susceptibility in southern Chinese children. Furthermore, miR-100 rs1834306 GG genotype had a greater genetic pathopoiesis in severe HSCR.

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Long non‐coding RNA FAL1 functions as a ceRNA to antagonize the effect of miR‐637 on the down‐regulation of AKT1 in Hirschsprung's disease

Abstract: These results illuminated that FAL1 may work as a ceRNA to modulate AKT1 expression via competitively binding to miR-637 in HSCR, suggesting that it may be clinically valuable as a biomarker of HSCR.

Cited by 51 publications

References 33 publications

Exosomal circRNAs: new players in the field of cholangiocarcinoma

Exosomal circRNAs: new players in the field of cholangiocarcinoma

<p>LINC00491 as a new molecular marker can promote the proliferation, migration and invasion of colon adenocarcinoma cells</p>

<p><em>miR-100</em> rs1834306 A>G Increases the Risk of Hirschsprung Disease in Southern Chinese Children</p>

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