Long Non-Coding RNAs Serve as Diagnostic Biomarkers of Preeclampsia and Modulate Migration and Invasiveness of Trophoblast Cells

Abstract

BackgroundLong non-coding RNAs (lncRNAs) have emerged as crucial regulators of tumor progression. However, the effects of lncRNAs in preeclampsia are not entirely clear. The aim of this study was to demonstrate the potential of lncRNAs to serve as biomarkers in preeclampsia.Material/MethodsThe RNA expression levels of lncRNAs (NR_027457, BC030099, AF037219, NR_024178, AF085938, G43016, G36948, NR_029420, NR_024015, AK002210, NR_026643, and AL049277) in the serum of patients with preeclampsia and in the serum of normal controls were measured by qRT-PCR. The area under the curve (AUC), the optimal cut-off values, the specificity, and the sensitivity of NR_027457, AF085938, G36948, and AK002210 were analyzed by receiver operating characteristic (ROC) curve analysis. We designed RNA interference species to suppress NR_027457 and G36948 and identified the roles of NR_027457 and G36948 in the functions of a trophoblast cell line (HTR-8/SVneo).ResultsThe qRT-PCR results indicated that NR_027457 and AF085938 were significantly up-regulated, whereas G36948 and AK002210 were significantly down-regulated in preeclampsia. We found that NR_027457, AF085938, G36948, and AK002210 had potential diagnostic value for the detection of preeclampsia. Furthermore, the levels of NR_027457, AF085938, G36948, and AK002210 in the serum of patients were significantly different before vs. after surgery. The silencing of NR_027457 inhibited the proliferation, migration, and invasion abilities of HTR-8/SVneo cells, while the silencing of G36948 promoted the proliferation, migration, and invasion abilities of HTR-8/SVneo cells.ConclusionsNR_027457, AF085938, G36948, and AK002210 can serve as potential diagnostic biomarkers in preeclampsia, and NR_027457 and G36948 might be involved in the pathogenesis of preeclampsia.

Plasmacytoma variant translocation 1 (PVT1) regulates trophoblast viability, proliferation, and migration and is downregulated in spontaneous abortion

“…The role of lncRNAs in regulation of cellular processes, including cell growth, proliferation, apoptosis, cell cycle, migration, invasion, differentiation, and others is well‐known . Several lncRNAs have been reported to affect cellular processes of trophoblast cells, including MVIH, TUG1, PVT1, Uc.187 , and MEG3 . In our study, we found that upregulation of PVT1 enhanced cell viability and promoted cell proliferation and migration of HRT8 cells, while PVT1 suppression repressed cell viability, cell proliferation, and migration.…”

Identification of Key circRNAs/lncRNAs/miRNAs/mRNAs and Pathways in Preeclampsia Using Bioinformatics Analysis

“…The mechanisms underlying PE might be caused by the dysregulation of LOC284100, LOC391533, and CEACAMP8 [ 22 ]. The lncRNAs AF085938 and NR_027457 were found to be upregulated significantly, while AK002210 and G36948 were downregulated in PE, and all of them had vital diagnostic values for the PE detection [ 39 ]. Overall, more studies focused on differential lncRNAs in PE, will result I more effective treatments found for PE.…”

An integrative bioinformatics analysis of microarray data for identifying hub genes as diagnostic biomarkers of preeclampsia

“…They found that misregulation of LOC391533, LOC284100 and CEACAMP8 might contribute to the mechanism underlying PE. In another lncRNA study, Luo and Li [19] indicated that NR_027457 and AF085938 were significantly up-regulated, whereas G36948 and AK002210 were significantly down-regulated in PE using the qRT-PCR method. They also found NR_027457, AF085938, G36948 and AK002210 had potential diagnostic value for the detection of PE.…”