Long Non-Coding RNAs Guide the Fine-Tuning of Gene Regulation in B-Cell Development and Malignancy

Dahl, Mette; Kristensen, Lasse Sommer; Grønbæk, Kirsten

doi:10.3390/ijms19092475

Cited by 36 publications

(31 citation statements)

References 183 publications

(205 reference statements)

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“…In the meanwhile, it can interact with miRNA, protein coding genes, and individual lncRNA to contribute to the progression of various cancers (25,26). Over the years, many types of lncRNAs have also been revealed as a prognostic factor and therapeutic targets in many kinds of cancers (27)(28)(29)(30)(31)(32). The lncRNA XLOC_013703 is 2336 nt in length, located in chr20 (start: 24930648; end: 24932983; − strand), consisting of 2 exons.…”

Section: Discussionmentioning

confidence: 99%

Retracted: Decreased expression of long noncoding RNA XLOC_013703 promotes cell growth via NF‐κB pathway in multiple myeloma

Huang

et al. 2019

IUBMB Life

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Long noncoding RNAs (lncRNAs) are dysregulated in cancer and involved in oncogenic or tumor inhibitory processes. The aim of the study was to investigate the expression pattern of lncRNA XLOC_013703 in multiple myeloma (MM) and to evaluate its biological role and potential significance. We found that XLOC_013703 was significantly decreased in CD138 positive plasma cells and serum of MM patients compared to normal controls, and the decreased XLOC_013703 expression was correlated with β2‐MG, serum‐free light chain (s‐FLC) and revised international staging system. RNA‐fluorescence in situ hybridization results revealed that XLOC_013703 was distributed both in the nucleus and in the cytoplasm of MM cells including H929, RPMI8226, and U266. Overexpression of XLOC_013703 inhibited the proliferation of U266 cells and blocked the cell cycle in G1 stage, thus contributing to MM cell apoptosis. By contrast, knockdown of XLOC_013703 promoted the growth of H929 cells. Western blot analysis confirmed that the expression of p‐IκBα and nuclear P65 was substantially increased in shRNA transfection groups compared to control groups, whereas overexpression of XLOC_013703 reduced these expressions. In conclusion, we confirmed that the decreased expression of a novel lncRNA, XLOC_013703, in MM. XLOC_013703 was involved in MM cell survival and proliferation via nuclear factor‐κB pathway which represents a potential therapeutic target for MM. © 2019 IUBMB Life, 71(9):1240–1251, 2019

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Section: Discussionmentioning

confidence: 99%

Retracted: Decreased expression of long noncoding RNA XLOC_013703 promotes cell growth via NF‐κB pathway in multiple myeloma

Huang

et al. 2019

IUBMB Life

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“…Long non–coding RNAs are largely recognized as important players in cancer disease [ 7 , 51 ], including haematological malignancies [ 16 , 52 , 53 ]. In addition, considering that lncRNA expression is tightly controlled and exhibits even higher cell specificity than proteins, lncRNAs are emerging as a promising class of biomarkers and/or potential therapeutic targets [ 54 , 55 , 56 , 57 ].…”

Section: Discussionmentioning

confidence: 99%

“…Several aberrantly expressed lncRNAs have been found associated with B–ALL [ 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 ]. For instance, Fernando et al [ 19 ] highlighted the over–expression of the lncRNA CASC15 with regulatory roles on the transcriptional factor SOX4, in paediatric RUNX–1 translocated B–ALL.…”

Section: Introductionmentioning

confidence: 99%

lncRNAs–mRNAs Co–Expression Network Underlying Childhood B–Cell Acute Lymphoblastic Leukaemia: A Pilot Study

Affinito

Pane

Smaldone

et al. 2020

Cancers

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Long non–coding RNAs (lncRNAs) are emerging as key gene regulators in the pathogenesis and development of various cancers including B lymphoblastic leukaemia (B–ALL). In this pilot study, we used RNA–Seq transcriptomic data for identifying novel lncRNA–mRNA cooperative pairs involved in childhood B–ALL pathogenesis. We conceived a bioinformatic pipeline based on unsupervised PCA feature extraction approach and stringent statistical criteria to extract potential childhood B–ALL lncRNA signatures. We then constructed a co–expression network of the aberrantly expressed lncRNAs (30) and protein–coding genes (754). We cross–validated our in–silico findings on an independent dataset and assessed the expression levels of the most differentially expressed lncRNAs and their co–expressed mRNAs through ex vivo experiments. Using the guilt–by–association approach, we predicted lncRNA functions based on their perfectly co–expressed mRNAs (Spearman’s correlation) that resulted closely disease–associated. We shed light on 24 key lncRNAs and their co–expressed mRNAs which may play an important role in B–ALL pathogenesis. Our results may be of clinical utility for diagnostic and/or prognostic purposes in paediatric B–ALL management.

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“…In the recent years, attention has been focused on long non-coding RNA (lncRNA), which are involved in many biological processes, such as transcriptional gene regulation, cell development and differentiation. Deregulation of lncRNAs has been demonstrated to be connected with tumor formation, progression and metastasis in many types of cancers, including hematological malignancies, although the information on a potential pathogenetic role in CLL is rather limited [2][3][4][5].…”

Section: Ofmentioning

confidence: 99%

NEAT1 Long Isoform Is Highly Expressed in Chronic Lymphocytic Leukemia Irrespectively of Cytogenetic Groups or Clinical Outcome

Ronchetti

Favasuli

Monti

et al. 2020

ncRNA

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The biological role and therapeutic potential of long non-coding RNAs (lncRNAs) in chronic lymphocytic leukemia (CLL) are still open questions. Herein, we investigated the significance of the lncRNA NEAT1 in CLL. We examined NEAT1 expression in 310 newly diagnosed Binet A patients, in normal CD19+ B-cells, and other types of B-cell malignancies. Although global NEAT1 expression level was not statistically different in CLL cells compared to normal B cells, the median ratio of NEAT1_2 long isoform and global NEAT1 expression in CLL samples was significantly higher than in other groups. NEAT1_2 was more expressed in patients carrying mutated IGHV genes. Concerning cytogenetic aberrations, NEAT1_2 expression in CLL with trisomy 12 was lower with respect to patients without alterations. Although global NEAT1 expression appeared not to be associated with clinical outcome, patients with the lowest NEAT1_2 expression displayed the shortest time to first treatment; however, a multivariate regression analysis showed that the NEAT1_2 risk model was not independent from other known prognostic factors, particularly the IGHV mutational status. Overall, our data prompt future studies to investigate whether the increased amount of the long NEAT1_2 isoform detected in CLL cells may have a specific role in the pathology of the disease.

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Long Non-Coding RNAs Guide the Fine-Tuning of Gene Regulation in B-Cell Development and Malignancy

Cited by 36 publications

References 183 publications

Retracted: Decreased expression of long noncoding RNA XLOC_013703 promotes cell growth via NF‐κB pathway in multiple myeloma

Retracted: Decreased expression of long noncoding RNA XLOC_013703 promotes cell growth via NF‐κB pathway in multiple myeloma

lncRNAs–mRNAs Co–Expression Network Underlying Childhood B–Cell Acute Lymphoblastic Leukaemia: A Pilot Study

NEAT1 Long Isoform Is Highly Expressed in Chronic Lymphocytic Leukemia Irrespectively of Cytogenetic Groups or Clinical Outcome

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