Long non-coding RNAs as biomarkers and therapeutic targets for ischemic stroke

Суфианова, Г. З.; Shumadalova, Alina; Wenhao, Yao; Gareev, Ilgiz

doi:10.1016/j.ncrna.2022.09.004

Cited by 5 publications

(4 citation statements)

References 58 publications

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“…m 6 A modification of RNA plays an important role in pathophysiological processes in the central nervous system, in which lncRNAs are key biomarkers in cerebral ischaemic disease [ 44 ], and the m 6 A modification status in lncRNAs may control the biological functions of lncRNAs [ 45 ]. Noncoding RNAs are considered novel therapeutic targets for ischaemic stroke [ 46 , 47 ]. However, five lncRNAs (Gm33408, Gm52552, Gm13110, Gm32335 and Gm31113) and two circRNAs (mmu_circ_0001258 and mmu_circ_0001558) were identified, and their specific functions and roles have not been reported, which may be a new entry point for cerebral I/R injury.…”

Section: Discussionmentioning

confidence: 99%

Identification of m6A methylation-related genes in cerebral ischaemia‒reperfusion of Breviscapus therapy based on bioinformatics methods

Wan,

Pei,

Wang

et al. 2023

BMC Med Genomics

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Background Cerebral ischaemia‒reperfusion (I/R) frequently causes late-onset neuronal damage. Breviscapine promotes autophagy in microvascular endothelial cells in I/R and can inhibit oxidative damage and apoptosis. However, the mediation mechanism of breviscapine on neuronal cell death is unclear. Methods First, transcriptome sequencing was performed on three groups of mice: the neuronal normal group (Control group), the oxygen-glucose deprivation/ reoxygenation group (OGD/R group) and the breviscapine administration group (Therapy group). Differentially expressed genes (DEGs) between the OGD/R and control groups and between the Therapy and OGD/R groups were obtained by the limma package. N6-methyladenosine (m6A) methylation-related DEGs were selected by Pearson correlation analysis. Then, prediction and confirmation of drug targets were performed by Swiss Target Prediction and UniProt Knowledgebase (UniProtKB) database, and key genes were obtained by Pearson correlation analysis between m6A-related DEGs and drug target genes. Next, gene set enrichment analysis (GSEA) and Ingenuity pathway analysis (IPA) were used to obtain the pathways of key genes. Finally, a circRNA-miRNA‒mRNA network was constructed based on the mRNAs, circRNAs and miRNAs. Results A total of 2250 DEGs between the OGD/R and control groups and 757 DEGs between the Therapy and OGD/R groups were selected by differential analysis. A total of 7 m6A-related DEGs, including Arl4d, Gm10653, Gm1113, Kcns3, Olfml2a, Stk26 and Tfcp2l1, were obtained by Pearson correlation analysis. Four key genes (Tfcp2l1, Kcns3, Olfml2a and Arl4d) were acquired, and GSEA showed that these key genes significantly participated in DNA repair, e2f targets and the g2m checkpoint. IPA revealed that Tfcp2l1 played a significant role in human embryonic stem cell pluripotency. The circRNA-miRNA‒mRNA network showed that mmu_circ_0001258 regulated Tfcp2l1 by mmu-miR-301b-3p. Conclusions In conclusion, four key genes, Tfcp2l1, Kcns3, Olfml2a and Arl4d, significantly associated with the treatment of OGD/R by breviscapine were identified, which provides a theoretical basis for clinical trials.

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Section: Discussionmentioning

confidence: 99%

Identification of m6A methylation-related genes in cerebral ischaemia‒reperfusion of Breviscapus therapy based on bioinformatics methods

Wan,

Pei,

Wang

et al. 2023

BMC Med Genomics

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“…To date, it has been demonstrated that not only coding mRNAs, but also various types of non-coding RNAs (ncRNAs), molecules with significant regulatory potential, are involved in the response to pathological effects [108][109][110][111][112]. One of the extensively studied types of ncRNAs is microRNAs (miRNAs).…”

Section: Micrornas In Is and Admentioning

confidence: 99%

Are Ischemic Stroke and Alzheimer’s Disease Genetically Consecutive Pathologies?

Filippenkov,

Khrunin,

Mozgovoy

et al. 2023

Biomedicines

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Complex diseases that affect the functioning of the central nervous system pose a major problem for modern society. Among these, ischemic stroke (IS) holds a special place as one of the most common causes of disability and mortality worldwide. Furthermore, Alzheimer’s disease (AD) ranks first among neurodegenerative diseases, drastically reducing brain activity and overall life quality and duration. Recent studies have shown that AD and IS share several common risk and pathogenic factors, such as an overlapping genomic architecture and molecular signature. In this review, we will summarize the genomics and RNA biology studies of IS and AD, discussing the interconnected nature of these pathologies. Additionally, we highlight specific genomic points and RNA molecules that can serve as potential tools in predicting the risks of diseases and developing effective therapies in the future.

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“…Due to their length of nucleotide chains, lncRNAs have the unique ability to take on many complex secondary and tertiary structures, allowing them to perform specific functions such as catalysis, metabolism, and cell differentiation. LncRNAs cannot code for protein, but can modulate gene expression at epigenetic (e.g., DNA methylation and histone modification), transcriptional (e.g., recruitment of transcription factors), and post-transcriptional (e.g., regulation of miRNA and mRNA stability) levels ( Guo and Yan, 2015 ; Ghafouri-Fard et al, 2020 ; Sufianova et al, 2022 ). CircRNAs are a class of regulatory ncRNAs with a closed structure of the ribose phosphate backbone.…”

Section: Introductionmentioning

confidence: 99%

Gastric juice non-coding RNAs as potential biomarkers for gastric cancer

et al. 2023

Self Cite

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Gastric cancer (GC), being one of the most common malignant human tumors, occupies the second position in the structure of mortality in men and women. High rates of morbidity and mortality in this pathology determine its extremely high clinical and social significance. Diagnosis and timely treatment of precancerous pathology is the main way to reduce morbidity and mortality, and early detection of GC and its adequate treatment improve prognosis. The ability to accurately predict the development of GC and start treatment on time, as well as the ability to determine the stage of the disease if the diagnosis is confirmed - non-invasive biomarkers can become the key to solving these and many other problems of modern medicine. One of the promising biomarkers being studied are non-coding RNAs, namely, miсroRNAs (miRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs). They are involved in a wide range of processes, including apoptosis, proliferation, differentiation, angiogenesis, which play a critical role in the development of GC oncogenesis. In addition, they are quite specific and stable due to their carriers (extracellular vesicles or Argonaute 2 protein) and can be detected in various human biological fluids, in particular gastric juice. Thus, miRNAs, lncRNAs, and circRNAs isolated from the gastric juice of GC patients are promising preventive, diagnostic and prognostic non-invasive biomarkers. This review article presents the characteristics of circulating or extracellular miRNAs, lncRNAs, and circRNAs in gastric juice, allowing their use in the GC preventive, diagnosis, prognosis and monitoring therapy.

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Long non-coding RNAs as biomarkers and therapeutic targets for ischemic stroke

Cited by 5 publications

References 58 publications

Identification of m6A methylation-related genes in cerebral ischaemia‒reperfusion of Breviscapus therapy based on bioinformatics methods

Identification of m6A methylation-related genes in cerebral ischaemia‒reperfusion of Breviscapus therapy based on bioinformatics methods

Are Ischemic Stroke and Alzheimer’s Disease Genetically Consecutive Pathologies?

Gastric juice non-coding RNAs as potential biomarkers for gastric cancer

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