Long non‑coding RNA TMPO‑AS1 serves as a tumor promoter in pancreatic carcinoma by regulating miR‑383‑5p/SOX11

Xue, Feng; Song, Xin; Zhang, Shuai; Niu, Meiwei; Cui, Yu; Wang, Ye; Zhao, Ting

doi:10.3892/ol.2021.12517

Cited by 9 publications

(8 citation statements)

References 24 publications

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“…A study by Li et al showed that the long intergenic noncoding RNA POU3F3 promotes ESCC tumor growth by interacting with EZH2 to increase the methylation of POU3F3 and reduce POU3F3 expression 35 . TMPO-AS1 expression has been reported to be upregulated in various cancers, including bladder cancer, pancreatic cancer, and lung adenocarcinoma 36 – 38 . However, the role of TMPO-AS1 in ESCC is less understood.…”

Section: Discussionmentioning

confidence: 99%

LncRNA TMPO-AS1 promotes esophageal squamous cell carcinoma progression by forming biomolecular condensates with FUS and p300 to regulate TMPO transcription

Luo

Liu

et al. 2022

Exp Mol Med

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Esophageal squamous cell carcinoma (ESCC) is one of the most life- and health-threatening malignant diseases worldwide, especially in China. Long noncoding RNAs (lncRNAs) have emerged as important regulators of tumorigenesis and tumor progression. However, the roles and mechanisms of lncRNAs in ESCC require further exploration. Here, in combination with a small interfering RNA (siRNA) library targeting specific lncRNAs, we performed MTS and Transwell assays to screen functional lncRNAs that were overexpressed in ESCC. TMPO-AS1 expression was significantly upregulated in ESCC tumor samples, with higher TMPO-AS1 expression positively correlated with shorter overall survival times. In vitro and in vivo functional experiments revealed that TMPO-AS1 promotes the proliferation and metastasis of ESCC cells. Mechanistically, TMPO-AS1 bound to fused in sarcoma (FUS) and recruited p300 to the TMPO promoter, forming biomolecular condensates in situ to activate TMPO transcription in cis by increasing the acetylation of histone H3 lysine 27 (H3K27ac). Targeting TMPO-AS1 led to impaired ESCC tumor growth in a patient-derived xenograft (PDX) model. We found that TMPO-AS1 is required for cell proliferation and metastasis in ESCC by promoting the expression of TMPO, and both TMPO-AS1 and TMPO might be potential biomarkers and therapeutic targets in ESCC.

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Section: Discussionmentioning

confidence: 99%

LncRNA TMPO-AS1 promotes esophageal squamous cell carcinoma progression by forming biomolecular condensates with FUS and p300 to regulate TMPO transcription

Luo

Liu

et al. 2022

Exp Mol Med

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“…A recent study conducted by Huang et al also approved that induction of miR-383 expression could inhibit growth and metastasis in PC [33]. Conversely, downregulation of miR-383 by TMPO-AS1 in lung adenocarcinoma and pancreatic carcinoma and MIR4435-2HG in head and neck squamous cell carcinoma leads to tumor progression [34][35][36].…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of curcumin against CD44± prostate cancer cells: Roles of miR-383 and miR-708

Panahizadeh

Vatankhah

Jeddi

et al. 2022

Preprint

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Cancer stem cells (CSCs) remaining in the tumor tissues after applying treatments may cause recurrence or metastasis of prostate cancer (PC). Curcumin has the promising potential to target CSCs. Here, we aim to evaluate the cytotoxic effects of curcumin on the expression of miR-383-5p and miR-708-5p and their target genes in CD44+ CSCs and CD44− non-CSCs isolated from the PC3 prostate cancer cell line. We used MTT assay to determine the optimal cytotoxic dose of curcumin on CD44± PC cells. Then, we assessed nuclear morphological changes using DAPi staining. We used Annexin V-FITC/PI to quantify apoptotic cell death. qRT-PCR was also used to detect miRNA and gene expression levels after curcumin treatment. Curcumin significantly enhanced the apoptosis in both CD44− and CD44+ PC cells in a dose-dependent manner (P-value < 0.05). The cytotoxicity of curcumin against CD44− cells (IC50 = 40.30 ± 2.32 µM) was found to be more effective than CD44+ cells (IC50 = 83.31 ± 2.91 µM). Also, curcumin promoted miR-383-5p and miR-708-5p overexpression while downregulating their target genes LDHA, PRDX3, and RAP1B, LSD1, respectively. Our findings indicate that curcumin, by promoting the expression of tumor suppressors, miR-383-5p and miR-708-5p, and inhibiting their target genes, induced its cytotoxicity against CD44± PC cells. We trust that curcumin could be established as a promising adjuvant therapy to current PC treatment options with more research in the clinical field.

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“…For example, lncRNA-FGD5-AS1 via sponging miR-383 accelerates the malignant characteristics of esophageal squamous cell carcinoma [38]. LncRNA-TMPO-AS1 promotes tumor growth, cell migration, and invasion in pancreatic carcinoma by regulating the miR-383/SOX11 axis [35]. Additionally, circRNA-CCS, via sponging miR-383, promotes lung cancer cell growth, metastasis, and predicted poor prognosis [14].…”

Section: Regulation Of Mir-383 Expressionmentioning

confidence: 99%

A Concise Review of MicroRNA-383: Exploring the Insights of Its Function in Tumorigenesis

Qian¹,

Xie²,

Sun³

et al. 2022

J. Cancer

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MicroRNAs (miRNAs) are small noncoding RNAs that commonly have 18-22 nucleotides and play important roles in the regulation of gene expression via directly binding to the 3'-UTR of target mRNAs. Approximately 50% of human genes are regulated by miRNAs and they are involved in many human diseases, including various types of cancers. Recently, microRNA-383 (miR-383) has been identified as being aberrantly expressed in multiple cancers, such as malignant melanoma, colorectal cancer, hepatocellular cancer, and glioma. Increasing evidence suggests that miR-383 participates in tumorigenic events including proliferation, apoptosis, invasion, and metastasis as well as drug resistance. Although downstream targets including CCND1, LDHA, VEGF, and IGF are illustrated to be regulated by miR-383, its roles in carcinogenesis are still ambiguous and the underlying mechanisms are still unclear. Herein, we review the latest studies on miR-383 and summarize its functions in human cancers and other diseases. The goal of this review is to provide new strategies for targeted therapy and further investigations.

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Long non‑coding RNA TMPO‑AS1 serves as a tumor promoter in pancreatic carcinoma by regulating miR‑383‑5p/SOX11

Cited by 9 publications

References 24 publications

LncRNA TMPO-AS1 promotes esophageal squamous cell carcinoma progression by forming biomolecular condensates with FUS and p300 to regulate TMPO transcription

LncRNA TMPO-AS1 promotes esophageal squamous cell carcinoma progression by forming biomolecular condensates with FUS and p300 to regulate TMPO transcription

Cytotoxicity of curcumin against CD44± prostate cancer cells: Roles of miR-383 and miR-708

A Concise Review of MicroRNA-383: Exploring the Insights of Its Function in Tumorigenesis

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