Long non‐coding RNA THRIL predicts increased acute respiratory distress syndrome risk and positively correlates with disease severity, inflammation, and mortality in sepsis patients

Wang, Yane; Fu, Xiangwei; Yu, Bo; Ai, Fen

doi:10.1002/jcla.22882

Cited by 29 publications

(22 citation statements)

References 15 publications

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“…Upregulated THRIL was found to predict increased risk of acute respiratory distress syndrome and positively correlates with disease severity, inflammation, and mortality in sepsis patients. 22 In this study, we found that lncRNA THRIL upregulated TNF-α in tumor tissue and serum of OS patients, which could be efficient diagnostic and prognostic biomarker. THRIL was also positively associated with CRP, PCT, TNF-α, and IL-1β levels in sepsis patients.…”

Section: Discussionmentioning

confidence: 70%

“…THRIL was also positively associated with CRP, PCT, TNF-α, and IL-1β levels in sepsis patients. 22 In Kawasaki disease, an acute inflammatory disease of childhood, THRIL was correlated with the severity of symptoms of patients. 13 In hypoxia-induced injuries, THRIL inhibition represents a protective effect against hypoxiainduced injuries in H9C2 cells.…”

Section: Discussionmentioning

confidence: 99%

“…13 In hypoxia-induced injuries, THRIL inhibition represents a protective effect against hypoxiainduced injuries in H9C2 cells. 22 However, THRIL RNA expression was significantly decreased in autism spectrum disorder (ASD) children, although TNF-α, IL-1β and IL-17 were significantly increased in ASD children. 23 THRIL was significantly decreased in patients with type 2 diabetes mellitus compared to control subjects, which were positively correlated with poor glycemic control, insulin resistance.…”

Section: Discussionmentioning

confidence: 99%

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<p>Upregulated lncRNA THRIL/TNF-α Signals Promote Cell Growth and Predict Poor Clinical Outcomes of Osteosarcoma</p>

Xu¹,

Jin²,

Yang³

et al. 2020

OTT

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Background: The immunosuppressive facet and tumorigenic role of TNF-α have been revealed in osteosarcoma (OS). Long noncoding RNA THRIL is identified to regulate TNF-α expression and participates in immune response. Thus, investigations on the clinical expression pattern of THRIL/TNF-α signal in OS would provide a potential target premise for OS patients. Methods: We collected OS (n=83), nontumor tissues (n=37) and serum samples (n=83 for OS and n=40 for healthy control) to determine the expressions and clinical significance of THRIL/TNF-α signal. Knockdown of THRIL in OS cell lines MG63 and Saos2 in vitro and in vivo was performed to confirm its function in the development of OS. Results: Elevated expression of THRIL was associated with increased TNF-α levels in OS tissues and serum samples. Combination of THRIL and TNF-α in tissues showed a more efficient diagnostic value for OS patients than either of them. Moreover, high-expressed THRIL was associated with larger tumor size, advanced Enneking stage and lung metastasis, whereas high TNF-α expression was found in patients with high histologic grade and patients who simultaneously harbor high THRIL and TNF-α showed the worst overall survival and metastasis-free survival. TNF-α signals increased OS cell vitalities in vitro but knockdown of THRIL inhibited TNF-α expressions, leading to impaired cell vitality, increased apoptosis and also downregulated epithelial to mesenchymal transition (EMT) phenotype and the ability of invasion, but these processes were restored by the treatment of TNF-α. The oncogenic role of THRIL/TNF-α signal was also confirmed in the xenograft model of MG63 cells. Conclusion: Overexpressed THRIL and TNF-α promoted OS progression with efficient diagnostic and prognostic value. THRIL/TNF-α signal supported cell growth and EMT phenotype of OS cells in vitro and in vivo.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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<p>Upregulated lncRNA THRIL/TNF-α Signals Promote Cell Growth and Predict Poor Clinical Outcomes of Osteosarcoma</p>

Xu¹,

Jin²,

Yang³

et al. 2020

OTT

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“…The overexpression of lncRNA THRIL in patients with inflammatory‐related diseases is reported by several studies . For example, it is reported that lncRNA THRIL is highly expressed in rheumatoid arthritis (RA) patients compared to healthy controls and discriminates RA patients from controls .…”

Section: Discussionmentioning

confidence: 99%

Up‐regulation of long non‐coding RNA THRIL in coronary heart disease: Prediction for disease risk, correlation with inflammation, coronary artery stenosis, and major adverse cardiovascular events

Shen

Zhou

2020

Clinical Laboratory Analysis

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This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. AbstractObjective: This study aimed to investigate the role of long non-coding RNA (lncRNA) THRIL in coronary heart disease (CHD) patients. Methods:A total of 420 patients who underwent coronary arteriography due to suspected symptoms of CHD were enrolled, in which 220 were diagnosed as CHD and 200 were set as control subjects. LncRNA THRIL in plasma samples of CHD patients

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“…LncRNA THRIL (TNFα and heterogenous nuclear ribonucleoprotein L (hnRNPL) related immunoregulatory LincRNA) is an enhancer of LPS-induced inflammatory [ 11 ]. In another study, THRIL was demonstrated to increase the risk of acute respiratory distress syndrome (ARDS) and was positively correlated with inflammatory responses, disease severity and mortality in sepsis patients [ 12 ]. Our bioinformatics analysis showed that THRIL may interact with miR-19a, which targets TNF-α [ 13 ], a critical player in sepsis [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

LncRNA THRIL is upregulated in sepsis and sponges miR-19a to upregulate TNF-α in human bronchial epithelial cells

Liu

Tian

et al. 2020

J Inflamm

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Background Long non-coding RNAs (lncRNAs) have been demonstrated to play critical roles in various diseases. Our bioinformatics analysis showed that lncRNA TNFα and heterogenous nuclear ribonucleoprotein L (hnRNPL) related immunoregulatory LincRNA (THRIL) may interact with miR-19a, which targets TNF-α. This study aimed to explore the role of THRIL, an enhancer of LPS-induced inflammatory, in sepsis. Methods Research subjects of the present study included 66 sepsis patients and 66 healthy volunteers. The expression levels of THRIL, miR-19a and TNF-α in plasma samples from these participants were determined by RT-qPCR. The interaction between THRIL and miR-19a was explored by performing overexpression experiments in human bronchial epithelial cells (HBEpCs). The roles of THRIL, miR-19a and TNF-α in regulating the apoptosis of HBEpCs were analyzed by cell apoptosis assay. Results We found that THRIL was upregulated in sepsis patients. THRIL is predicted to interact with miR-19a, and the interaction was confirmed by dual-luciferase activity assay. However, THRIL and miR-19a did not affect the expression of each other. Instead, overexpression of THRIL resulted in the increased expression levels of TNF-α, a downstream target of miR-19a in HBEpCs. In HBEpCs, LPS treatment induced the overexpression of THRIL. Cell apoptosis analysis showed that overexpression of THRIL and TNF-α promoted the apoptosis of HBEpCs induced by LPS, while overexpression of miR-19a played an opposite role. Overexpression of THRIL attenuated the effects of overexpression of miR-19a. Conclusion Therefore, THRIL is upregulated in sepsis and may sponge miR-19a to upregulate TNF-α, thereby promoting lung cell apoptosis.

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Long non‐coding RNA THRIL predicts increased acute respiratory distress syndrome risk and positively correlates with disease severity, inflammation, and mortality in sepsis patients

Cited by 29 publications

References 15 publications

<p>Upregulated lncRNA THRIL/TNF-α Signals Promote Cell Growth and Predict Poor Clinical Outcomes of Osteosarcoma</p>

<p>Upregulated lncRNA THRIL/TNF-α Signals Promote Cell Growth and Predict Poor Clinical Outcomes of Osteosarcoma</p>

Up‐regulation of long non‐coding RNA THRIL in coronary heart disease: Prediction for disease risk, correlation with inflammation, coronary artery stenosis, and major adverse cardiovascular events

LncRNA THRIL is upregulated in sepsis and sponges miR-19a to upregulate TNF-α in human bronchial epithelial cells

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