Long non-coding RNA SOX2OT promotes stemness characteristics and drug-resistance of bladder cancer cells by modulating SOX2

Zhan, Y.; Chen, Z.; He, S.; Li, X.; Zhou, L.

doi:10.1016/s1569-9056(19)31186-8

Cited by 1 publication

(2 citation statements)

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“…There is some supporting evidence highlighting SOX2OT association with drug resistance to other chemotherapy agents with a different mechanism. It has been reported that SOX2OT inhibition can restrict the bladder cancer stem cell (CD44+, ALDH1+) self-renewal and cisplatin resistance by SOX2 regulation [28]. Wang et al showed that EGCG (polyphenol derived from green tea), which targets SOX2OT, can synergize doxorubicin toxicity in osteosarcoma cells by autophagy and stemness inhibition [27].…”

Section: Discussionmentioning

confidence: 99%

“…One of the master modulators of pluripotency in cancer stem cells, SOX2 is located in an intronic region of SOX2OT long non-coding RNA. SOX2OT is mapped on a highly conserved region in chromosome 3q26.3 in humans [21,22] and has various cellular functions in embryogenesis and neuronal development [23], cancer cell proliferation [24,25], metastasis [26], and drug resistance [27,28]. Previous studies support overexpression of SOX2OT in tumor tissues of the breast [29], esophageal [30], lung [24,31], and hepatocellular [26].…”

Section: Introductionmentioning

confidence: 99%

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SOX2OT lncRNA Inhibition Suppresses the Stemness Characteristics of Esophageal Tumorspheres

Haghi

Jazi

Khosravi

et al. 2022

ncRNA

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Background: SOX2OT is a novel cancer associated long non-coding RNA (LncRNA) with higher expression in variable tumor tissues, including esophageal squamous cell carcinoma (ESCC). It also plays an important function in embryonic neuronal development. Regarding its function in both stemness and carcinogenesis, here, we aimed to investigate its expression and function in tumorspheres of the esophagus using the RNAi method. Material & Methods: Two esophageal squamous cancer cells (ESCC): KYSE30 and YM1 cells were used for sphere enrichment. Cells were transfected with SOX2OT targeting and control siRNA. The size and the number of spheres were measured using light microscopy. Gene expression of the pluripotency genes was measured by qRT-PCR and docetaxel chemoresistance was assessed by MTS viability assay. Results: Our findings showed that ESCC tumorspheres overexpress SOX2OT gene along with other stemness genes (SOX2, OCT4A, and Nanog) compared to their original cancer cells. RNAi experiments indicated that SOX2OT knockdown can suppress the stemness-related gene expression, sphere formation ability (both size and number), and docetaxel resistance as three of the main cancer stem cell characteristics of tumorspheres. Conclusion: Altogether our results showed the regulatory role of SOX2OT in pluripotency and stemness in ESCC tumorspheres. Our results suggest a potential application of SOX2OT inhibition in combination with docetaxel for ESCC inhibition in vitro.

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Section: Discussionmentioning

confidence: 99%