Long non‑coding RNA SNHG9 inhibits ovarian cancer progression by sponging microRNA‑214‑5p

Chen, Gaoyang; Zhang, Zhisheng; Chen, Yu; Li, Yan

doi:10.3892/ol.2020.12341

Cited by 9 publications

(5 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SNHG9 is a lncRNA whose coding gene is located on chromosome 17 and has a total length of approximately 416 base pairs. It was found to be associated with the development and progression of various cancers, including pancreatic cancer, glioblastoma, lung cancer, and ovarian cancer ( Zhang et al, 2018 ; Lin et al, 2020 ; Chen et al, 2021 ; Wang et al, 2022 ). To date, there are limited investigations on SNHG9 in EV-D68 infection.…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA SNHG9 regulates viral replication in rhabdomyosarcoma cells infected with enterovirus D68 via miR-150-5p/c-Fos axis

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

BackgroundThe Enterovirus D68 (EV-D68) epidemic has increased knowledge of the virus as a pathogen capable of causing serious respiratory and neurological illnesses. It has been shown that long noncoding RNAs (lncRNAs) regulate viral replication and infection via multiple mechanisms or signaling pathways. However, the precise function of lncRNAs in EV-D68 infection remains unknown.MethodsThe differential expression profiles of lncRNA in EV-D68-infected and uninfected rhabdomyosarcoma (RD) cells were studied using high-throughput sequencing technology. The knockdown through small interfering RNA (siRNA) and overexpression of lncRNA SNHG9 (small ribonucleic acid host gene 9) were applied to investigate how lncRNA SNHG9 regulates EV-D68 propagation. The targeted interactions of lncRNA SNHG9 with miR-150-5p and miR-150-5p with c-Fos were validated using dual luciferase reporter system. LncRNA SNHG9 knockdown and miR-150-5p inhibitor were co-transfected with RD cells. QRT-PCR and western blot were used to detect RNA and protein levels, of c-Fos and VP1, respectively. Median tissue culture infectious dose (TCID50) was applied to detect viral titers.ResultsThe results demonstrated that a total of 375 lncRNAs were highly dysregulated in the EV-D68 infection model. In the EV-D68 infection model, lncRNA SNHG9 and c-Fos were increased in EV-D68-infected RD cells. However, the expression level of miR-150-5p was downregulated. In addition, overexpression of SNHG9 in RD cells resulted in decreased viral replication levels and viral titers following infection with EV-D68, and further experiments revealed that overexpression of SNHG9 inhibited the viral replication by targeting increased miR-150-5p binding and significantly increased c-Fos expression in RD cells.ConclusionOur findings indicate that the SNHG9/miR-150-5p/c-Fos axis influences EV-D68 replication in host cells and that SNHG9 may be a possible target for anti-EV-D68 infection therapies.

show abstract

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA SNHG9 regulates viral replication in rhabdomyosarcoma cells infected with enterovirus D68 via miR-150-5p/c-Fos axis

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Accumulating evidence has suggested that the SNHG9 may serve a role in multiple types of cancer 12, 17–19 . For instance, SNHG9 was identified as a prognostic marker for PC.…”

Section: Discussionmentioning

confidence: 99%

Knockdown of long non‐coding RNA small nucleolar RNA host gene 9 or hexokinase 2 both suppress endometrial cancer cell proliferation and glycolysis

Wang

Huang

Lin

et al. 2021

J of Obstet and Gynaecol

View full text Add to dashboard Cite

Aim Endometrial cancer (EC) is a common type of malignant gynecological cancer. Small nucleolar RNA host gene 9 (SNHG9) has been discovered to serve a role in several types of cancer; however, the role of SNHG9 in EC remains unclear. The present study aimed to investigate the effects of lncRNA SNHG9 on cell proliferation and glycolysis in EC cells. Methods SNHG9 and hexokinase 2 (HK2) mRNA expression levels were measured by reverse transcription‐quantitative PCR. Glucose consumption and lactate production were detected by the glycolysis cell‐based assay kit. Cell Counting Kit‐8 and colony formation assays were conducted to detect cell proliferation. The knockdown experiments of SNHG9 and HK2 were carried out by transfection of corresponding small interference RNAs (siRNA). The SNHG9‐overexpressed plasmid was transfected into the cells to upregulate SNHG9. HK2 protein levels were analyzed by western blotting assay. Results SNHG9 expression levels were significantly upregulated in EC tissues and cells. The knockdown of SNHG9 subsequently effectively attenuated cell proliferation and glycolysis in vitro, while SNHG9 overexpression reported the opposite effects. Notably, the transfection of 2‐DG partially reversed the promoting effect of SNHG9 on glycolysis. Downregulation of HK2 markedly decreased cell proliferation and glycolysis in EC cells antagonized SNHG9. Conclusion Either downregulation of SNHG9 or HK2 inhibits EC cell proliferation and glycolysis via repressing EC cell proliferation and glycolysis.

show abstract

“…different cell processes such as cell migration, growth, invasion, apoptosis, and differentiation in OC (Wang et al, 2019 ). For example, SNHG9 was shown to serve as an anticancer biomarker by regulating miR-214-5p (Chen et al, 2021 ). LINC01969 was confirmed to be a cancer-promoting biological marker via the miR-144-5p / LARP1 axis (Chen et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Four Prognosis-Associated lncRNAs Serve as Biomarkers in Ovarian Cancer

et al. 2021

View full text Add to dashboard Cite

Long non-coding RNAs (lncRNAs) play crucial roles in ovarian cancer (OC) development. However, prognosis-associated lncRNAs (PALs) for OC have not been completely elucidated. Our study aimed to identify the PAL signature of OC. A total of 663 differentially expressed lncRNAs were identified in the databases. According to the weighted gene coexpression analysis, the highly correlated genes were clustered into seven modules related to the clinical phenotype of OC. A total of 25 lncRNAs that were significantly related to overall survival were screened based on univariate Cox regression analysis. The prognostic risk model constructed contained seven PALs based on the parameter λmin, which could stratify OC patients into two risk groups. The results showed that the risk groups had different overall survival rates in both The Cancer Genome Atlas (TCGA) and two verified Gene Expression Omnibus (GEO) databases. Univariate and multivariate Cox regression analyses confirmed that the risk model was an independent risk factor for OC. Gene enrichment analysis revealed that the identified genes were involved in some pathways of malignancy. The competitive endogenous RNA (ceRNA) network included five PALs, of which four were selected for cell function assays. The four PALs were downregulated in 33 collected OC tissues and 3 OC cell lines relative to the control. They were shown to regulate the proliferative, migratory, and invasive potential of OC cells via Cell Counting Kit-8 (CCK-8) and transwell assays. Our study fills the gaps of the four PALs in OC, which are worthy of further study.

show abstract

Long non‑coding RNA SNHG9 inhibits ovarian cancer progression by sponging microRNA‑214‑5p

Cited by 9 publications

References 33 publications

Long non-coding RNA SNHG9 regulates viral replication in rhabdomyosarcoma cells infected with enterovirus D68 via miR-150-5p/c-Fos axis

Long non-coding RNA SNHG9 regulates viral replication in rhabdomyosarcoma cells infected with enterovirus D68 via miR-150-5p/c-Fos axis

Knockdown of long non‐coding RNA small nucleolar RNA host gene 9 or hexokinase 2 both suppress endometrial cancer cell proliferation and glycolysis

Four Prognosis-Associated lncRNAs Serve as Biomarkers in Ovarian Cancer

Contact Info

Product

Resources

About