Long non-coding RNA SNHG20 promotes non-small cell lung cancer cell proliferation and migration by epigenetically silencing of P21 expression

Chen, Zhenyao; Chen, Xin; Chen, Ping; Yu, Shanxun; Nie, Fengqi; Lu, Binfeng; Zhang, Te; Zhou, Yue; Chen, Qinnan; Wei, Chenchen; Wang, Wei; Wang, Zhaoxia

doi:10.1038/cddis.2017.484

Cited by 93 publications

(84 citation statements)

References 36 publications

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“…Existing evidence indicates that long noncoding RNAs (lncRNAs) are involved in the occurrence and development of liver diseases including NAFLD and HCC . Small nucleolar RNA host gene 20 (SNHG20) is a recently discovered lncRNA implicated in multiple cancers . Our preliminary results confirmed that SNHG20 expression was increased in liver tissues in the NALFD‐HCC (NALFD accompanied by HCC) tumor group compared with the NALFD group, both in human and experimental mouse livers.…”

Section: Introductionsupporting

confidence: 71%

Silencing of lncRNA SNHG20 delays the progression of nonalcoholic fatty liver disease to hepatocellular carcinoma via regulating liver Kupffer cells polarization

Wang

et al. 2019

IUBMB Life

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This study aims to investigate the role of long noncoding RNA SNHG20 in the progression of nonalcoholic fatty liver disease (NALFD) to hepatocellular carcinoma (HCC), and to elucidate whether polarization of Kupffer cells (KCs, liver macrophages) was involved in this process. Mouse NALFD was induced by 16 weeks of high-fat diet (HFD) feeding. Mouse NALFD-HCC was induced by 36 weeks of HFD feeding (from 1 week to 36 weeks) and 20 weeks of diethyl nitrosamine (DEN) administration (from 17 weeks to 36 weeks). The LV-shRNA-and LV-sh-SNHG20-infected RAW264.7 cells were injected into the NALFD mice followed by DEN treatment to evaluate the role of SNHG20 in regulating the progression of NALFD to HCC in mice. The proportion of M1 and M2 macrophages was determined by flow cytometry. The levels of M1-related inducible nitric oxide synthase (iNOS) and tumor necrosis factor-α and M2-related Arg-1 and interleukin (IL)-10 were also examined. SNHG20 expression was decreased in NALFD but increased in NALFD-HCC, both in human and experimental mouse livers. Furthermore, human and mouse NALFD-HCC KCs displayed decreased M1/M2 ratio compared with NALFD KCs. Moreover, SNHG20 overexpression induced M2 polarization through activating STAT6, whereas SNHG20 silencing suppressed M1 polarization in RAW264.7 macrophages and delayed the progression of NALFD to HCC in mice. SNHG20 may facilitate the progression of NALFD to HCC via inducing liver KCs M2 polarization via STAT6 activation.

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Section: Introductionsupporting

confidence: 71%

Silencing of lncRNA SNHG20 delays the progression of nonalcoholic fatty liver disease to hepatocellular carcinoma via regulating liver Kupffer cells polarization

Wang

et al. 2019

IUBMB Life

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“…Then, Liu et al further presented evidence that levels of SNHG20 expression were increased in 96 pairs of hepatocellular carcinoma tissues and four cancer cell lines compared with adjacent normal tissues and normal human liver cell line, respectively . Moreover, Chen et al reported that SNHG20 expression was elevated in non‐small cell lung cancer samples compared with normal samples . In colorectal cancer, Li et al indicated that levels of SNHG20 expression were up‐regulated in tumor tissues compared to corresponding normal tissues from 107 .…”

Section: Discussionmentioning

confidence: 99%

“…In order to further explore the clinical significance of SNHG20 in the progression of osteosarcoma, we assessed the association between SNHG20 expression and clinical parameters of osteosarcoma cases, and observed high‐expression of SNHG20 was obviously associated with Enneking stage, distant metastasis, and histological grade. In non‐small cell lung cancer patients, Chen et al indicated levels of SNHG20 were correlated with advanced TNM stage and big tumor size . Moreover, Guo et al demonstrated that SNHG20 overexpression was related to bigger tumor size, advanced FIGO stage, and existent lymph node metastasis in patients with cervical cancer .…”

Section: Discussionmentioning

confidence: 99%

“…Recently, the prognostic value of SNHG20 has been suggested in hepatocellular carcinoma , lung cancer , colorectal cancer and cervical cancer . Liu et al found that patients with high level of SNHG20 expression had shorter overall survival time and disease‐free survival time than those with low level of SNHG20 expression, and high‐expression of SNHG20 was an independent prognostic factor for overall survival in hepatocellular carcinoma patients .…”

Section: Discussionmentioning

confidence: 99%

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LncRNA SNHG20 is associated with clinical progression and enhances cell migration and invasion in osteosarcoma

Zhang

Ju²,

Zhang³

et al. 2018

IUBMB Life

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SNHG20 is a novel lncRNA which has been showed to be overexpressed and functions as oncogenic lncRNA in several types of human cancer. The role of SNHG20 in osteosarcoma is still unknown. The purpose of our study was to identify the clinical value and biological function of SNHG20 in osteosarcoma. Our results indicated SNHG20 was overexpressed in osteosarcoma tissues and cell lines compared with paired non-tumor tissues and normal human osteoblast cell line, respectively. Moreover, we observed high-expression of SNHG20 was obviously associated with Enneking stage, distant metastasis, and histological grade. SNHG20 expression was negatively associated with overall survival, and high-expression of SNHG20 was an independent unfavorable prognostic factor in osteosarcoma patients. Furthermore, SNHG20 promoted osteosarcoma cell migration and invasion through modulating epithelial-mesenchymal transition -associated genes. In conclusion, SNHG20 is an independent unfavorable prognostic factor in osteosarcoma patients, and functions as an oncogenic lncRNA to modulate tumor cell migration and invasion. © 2018 IUBMB Life, 70(11):1115-1121, 2018.

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“…It has been previously reported that lncRNAs could bind to EZH2 and epigenetically silence the downstream target genes [26,28]. We therefore performed RNA immunopreciptation (RIP) analysis to examine whether SNHG6 could bind to EZH2.…”

Section: Snhg6 Inhibits P21 Expression In Colorectal Cancer Cellsmentioning

confidence: 99%

SNHG6 Promotes Tumor Growth via Repression of P21 in Colorectal Cancer

Qiu

Qiu³

et al. 2018

Cell Physiol Biochem

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Background/Aims: SNHG6 (Small Nucleolar RNA Host Gene 6) is a novel non-coding RNA (ncRNA) and its cellular function is largely unknown. Methods: Cell Counting Kit-8 (CCK-8) cell growth assay, colony formation and flow cytometry were used to determine colorectal cancer cell proliferation, cell cycle progression and apoptosis in vitro. The xenograft tumor formation assay in nude mice was established to evaluate tumor growth in vivo. RNA immunopreciptation (RIP) analysis was performed to examine whether SNHG6 could bind to EZH2 (enhancer of zeste 2 polycomb repressive complex 2 subunit), and chromatin immunoprecipitation (ChIP) assay was conducted to examine whether SNHG6 could repress p21 transcription by recruiting EZH2 to the p21 promoter. Results: Here we found that SNHG6 was upregulated and its expression levels were positively correlated with advanced tumor stage in colorectal cancer. Survival analysis suggested that higher expression of SNHG6 predicted poor prognosis in patients with colorectal cancer. Functional studies indicated that SNHG6 could promote cell proliferation via a direct suppression of p21 expression in colorectal cancer cells. Moreover, SNHG6 repressed p21 transcription through recruiting EZH2 to the p21 promoter in colorectal cancer cells. Conclusion: Taken together, our study demonstrates that SNHG6 promotes tumor growth via repression of p21 in colorectal cancer, which may provide a promising target for novel anticancer therapeutics.

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Long non-coding RNA SNHG20 promotes non-small cell lung cancer cell proliferation and migration by epigenetically silencing of P21 expression

Cited by 93 publications

References 36 publications

Silencing of lncRNA SNHG20 delays the progression of nonalcoholic fatty liver disease to hepatocellular carcinoma via regulating liver Kupffer cells polarization

Silencing of lncRNA SNHG20 delays the progression of nonalcoholic fatty liver disease to hepatocellular carcinoma via regulating liver Kupffer cells polarization

LncRNA SNHG20 is associated with clinical progression and enhances cell migration and invasion in osteosarcoma

SNHG6 Promotes Tumor Growth via Repression of P21 in Colorectal Cancer

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