Long non-coding RNA PVT1 serves as a competing endogenous RNA for miR-186-5p to promote the tumorigenesis and metastasis of hepatocellular carcinoma

Lan, Tian; Xia, Yan; Li, Zhuo; Xu, Xin; Mao, Qinan; Ma, Weijie; Hong, Zhenfei; Chen, Xi; Yuan, Yufeng

doi:10.1177/1010428317705338

Cited by 53 publications

(46 citation statements)

References 20 publications

(27 reference statements)

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“…Higher PVT1 expression level is associated with tumor progression and predicts recurrence in HCC patients . Tian reported that PVT1 promotes the tumorigenesis and metastasis of hepatocellular carcinoma by acting as a competing endogenous RNA for miR‐186‐5p , which is consistent with our analyses results. Moreover, our finding also reveals that knockdown of PVT1 impedes HCC cells invasion.…”

Section: Discussionsupporting

confidence: 91%

A comprehensive genome‐wide analysis of long noncoding RNA expression profile in hepatocellular carcinoma

Cui

Zhang

et al. 2017

Cancer Medicine

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Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide, especially in East Asia and China. Long noncoding RNAs (lncRNAs) are emerging as critical regulators that may be involved in the development and progression of cancers in humans. However, the contributions of lncRNAs to HCC development, metastasis, and recurrence remain largely unknown. In this study, we comprehensively investigated lncRNA expression profile in HCC and normal tissues using TCGA RNA sequencing data, one RNA sequencing dataset, and two microarray datasets from GEO. By analyzing these four datasets, we identified hundreds of expression‐dysregulated lncRNAs in HCC tissues compared with normal tissues. Genomic copy number variation analysis showed that many of those lncRNAs disorder are related to the copy number amplification or deletion. Moreover, several lncRNAs expression levels are associated with HCC patients' overall and recurrence‐free survival, such as RP1‐228H13.5, TMCC1‐AS1, LINC00205, and RP11‐307C12.11. Furthermore, we identified two lncRNAs termed PVT1 and SNHG7 that may be involved in HCC cells metastasis by comparing lncRNAs expression profiles between early recurrence HCC tissues with metastasis and late recurrence HCC tissues without metastasis. Finally, loss‐of‐function assays confirmed that knockdown of SNHG7 and PVT1 impaired HCC cells invasion. Taken together, these findings may provide a valuable resource for further identification of novel biomarkers and therapeutic targets for HCC patients.

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Section: Discussionsupporting

confidence: 91%

A comprehensive genome‐wide analysis of long noncoding RNA expression profile in hepatocellular carcinoma

Cui

Zhang

et al. 2017

Cancer Medicine

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“…So far, mounting evidences have indicated that lncRNAs had close connection with the HCC progression . For instance, Lan et al have reported that HCC tumorigenesis was accelerated by lncRNA PVT1 . In addition, Lu et al proved that overexpressed lncRNA HOXD‐AS1 was a critical regulator of metastasis in HCC .…”

Section: Discussionmentioning

confidence: 99%

“…26 For instance, Lan et al have reported that HCC tumorigenesis was accelerated by lncRNA PVT1. 27 In addition, Lu et al proved that overexpressed lncRNA HOXD-AS1 was a critical regulator of metastasis in HCC. 28 CRNDE has been identified to promote tumour development, 9,29 and its function in HCC remains had been preliminarily revealed in some research, such as Chen et al revealed that CRNDE promotes hepatic carcinoma cell proliferation, migration and invasion by suppressing miR-384.…”

Section: Discussionmentioning

confidence: 99%

Long non‐coding RNA CRNDE promotes the proliferation, migration and invasion of hepatocellular carcinoma cells through miR‐217/MAPK1 axis

Wang

Guo

et al. 2018

J Cellular Molecular Medi

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Hepatocellular carcinoma (HCC) is an invasive malignant tumour and the second major cause of cancer‐related deaths over the world. CRNDE and miR‐217 are non‐coding RNAs which play critical roles in cell growth, proliferation, migration. Mitogen‐activated protein kinase 1 (MAPK1) also participates in cancer cell process. Hence, this study aimed at investigating the effect of CRNDE on migration and invasion of HCC and figuring out the role of miR‐217 and MAPK1 in this process. The overexpression of CRNDE was demonstrated by a microarray‐based lncRNA profiling study. CRNDE expression in HCC was verified by qRT‐PCR. MTT assay and BrdU staining were applied to detect cell proliferation level. Transwell assay was utilized to examine cell migration and invasiveness abilities. Wound healing assay was performed for further exploration of cell migration capacity. MiR‐217 was predicted by bioinformatics. The dual luciferase reporter assay was performed to corroborate the targeting relationship between CRNDE, miR‐217 and MAPK1. MAPK1, the downstream target of miR‐217, was predicted using bioinformatics and was further confirmed by qRT‐PCR and Western blot. The interaction between CRNDE, miR‐217 and MAPK1 was studied by qRT‐PCR, Western blot, MTT, BrdU, transwell assay and wound healing assay. CRNDE was up‐regulated in HCC tissues and HCC cell lines. The high expression of CRNDE facilitated cell proliferation, migration and invasion, while the inhibited one affected on the contrary. MiR‐217, negatively correlated with CRNDE expression, was the target of CRNDE and was more lowly expressed in HCC. With the high expression of miR‐217, HCC cell proliferation, migration and invasion were suppressed. MAPK1, the possible target of miR‐217, was negatively correlated with miR‐217 but positively correlated with CRNDE and had the same effect in HCC formation process as CRNDE. Long non‐coding RNA CRNDE promotes the proliferation, migration and invasion of HCC cells through miR‐217/MAPK1 axis.

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“…Ma et al revealed that PVT1 was highly expressed in glioma vascular endothelial cells, which increased the expression of Beclin1 and Atg7 by targeting miR-186, thereby promoting cell proliferation, migration and angiogenesis [44]. Lan et al discovered that PVT1 acts as an endogenous sponge of miR-186-5p to reduce its inhibitory effect on yes-associated protein 1, promoting the tumorigenesis of hepatocellular carcinoma [45]. Although advances were made in the understanding of the role of PVT1 in tumors, its precise molecular mechanisms underlying its function are still unclear.…”

Section: Discussionmentioning

confidence: 99%

Prognostic Significance of LncRNA PVT1 and Its Potential Target Gene Network in Human Cancers: a Comprehensive Inquiry Based Upon 21 Cancer Types and 9972 Cases

Qin

Lin

et al. 2018

Cell Physiol Biochem

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Background/Aims: Whether the level of long noncoding RNA plasmacytoma variant translocation 1 gene (lncRNA PVT1) expression influences the clinical development and outcome of human cancers has not been thoroughly elucidated to date. Inconsistencies still exist regarding the associations between PVT1 and the clinicopathological features, including patient survival data. Additionally, the regulatory mechanism of PVT1 among human cancers remains unclear. Methods: we conducted a comprehensive inquiry to verify the implication of PVT1 expression in cancer patients by conducting a meta-analysis of 19 selected studies and The Cancer Genome Atlas (TCGA) database to examine the relationship between PVT1 expression and both the prognosis and clinicopathological features of cancer patients using STATA 12.0. In addition, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of the potential mRNA target genes of PVT1 gathered from TANRIC and Multi Experiment Matrix (MEM) were performed. Results: The level of PVT1 expression in tumor tissues was higher than in paired non-cancer tissues and was significantly associated with a poorer prognosis in cancer patients. Additionally, overexpression of PVT1 was significantly correlated with histological differentiation, tumor (T) classification, lymph node (N) classification and TNM stages. Furthermore, a total of 462 validated target genes were identified, and the GO and KEGG analyses demonstrated that the validated targets of PVT1 were significantly enriched in several pathways, including the GnRH signaling pathway, the Cytokine-cytokine receptor interaction pathway, the Inflammatory mediator regulation of TRP channels pathway, and the Neuroactive ligand-receptor interaction pathway. Conclusion: PVT1 may serve as a potential biomarker associated with the progression and prognosis of human cancers.

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Long non-coding RNA PVT1 serves as a competing endogenous RNA for miR-186-5p to promote the tumorigenesis and metastasis of hepatocellular carcinoma

Cited by 53 publications

References 20 publications

A comprehensive genome‐wide analysis of long noncoding RNA expression profile in hepatocellular carcinoma

A comprehensive genome‐wide analysis of long noncoding RNA expression profile in hepatocellular carcinoma

Long non‐coding RNA CRNDE promotes the proliferation, migration and invasion of hepatocellular carcinoma cells through miR‐217/MAPK1 axis

Prognostic Significance of LncRNA PVT1 and Its Potential Target Gene Network in Human Cancers: a Comprehensive Inquiry Based Upon 21 Cancer Types and 9972 Cases

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