Long Non-coding RNA PVT1 Promotes Cell Proliferation and Migration by Silencing ANGPTL4 Expression in Cholangiocarcinoma

Abstract

Cholangiocarcinoma (CCA) is the most common biliary tract malignancy, with a low survival rate and limited treatment options. Long non-coding RNAs (lncRNAs) have recently been verified to have significant regulatory functions in many kinds of human cancers. It was discovered in this study that the lncRNA PVT1, whose expression is significantly elevated in CCA, could be a molecular marker of CCA. Experiments indicated that PVT1 knockdown greatly inhibited cell migration and proliferation in vitro and in vivo. According to RNA sequencing (RNA-seq) analysis, PVT1 knockdown dramatically influenced target genes associated with cell angiogenesis, cell proliferation, and the apoptotic process. RNA immunoprecipitation (RIP) analysis demonstrated that, by binding to epigenetic modification complexes (PRC2), PVT1 could adjust the histone methylation of the promoter of ANGPTL4 (angiopoietin-like 4) and, thus, promote cell growth, migration, and apoptosis progression. The data verified the significant functions of PVT1 in CCA oncogenesis, and they suggested that PVT1 could be a target for CCA intervention.

Long noncoding RNA SNHG6 promotes proliferation and angiogenesis of cholangiocarcinoma cells through sponging miR-101-3p and activation of E2F8

“…Recently, lncRNAs have gained great attention as its role in CCA 15 . Several known lncRNAs were reported to be dysregulated in CCA tissues and involved in the CCA development and progression, such as DANCR 16 , PVT1 17 , and RMRP 18 . Recent studies have reported that SNHG6 is highly expressed in variety of cancer tissues (except CCA) and .…”

Expression of long non-coding RNA ANRIL predicts a poor prognosis in intrahepatic cholangiocarcinoma

“…APOC1P1, PVT1) possibly regulating inflammation and oxidative stress in malignant cholangiocytes 10,11 . Notably, pvt1 oncogene (PVT1) was shown to promote cell proliferation and migration by epigenetically silencing angiopoietin like 4 (ANGPTL4), a tumor suppressor gene candidate 12 . Cyclin dependent kinase inhibitor 1A (CDKN1A), another well-described tumor suppressor gene, was shown to be epigenetically silenced in CCA through the interaction of small nucleolar RNA host gene 1 (SNHG1) lncRNA with enhancer of zeste 2 (EZH2), the catalytic subunit of the polycomb repressive complex 2 (PRC2) 13 .…”

Functions and roles of long noncoding RNA in cholangiocarcinoma

“…These findings indicate that SPRY4-IT1 exhibits tremendous potentiality for the diagnosis and treatment of CCA and likely functions as a novel diagnostic biomarker and intervention target. experiment was also conducted to prove its carcinogenic function (Han et al, 2018;Yu, W. D. et al, 2018;Yu, Y. et al, 2018aYu, Y. et al, , 2018b. Similar to the above, small nucleolar RNA host gene 1 (SNHG1), which is situated at 11q12.3 region of the human chromosome Zhang, D. et al, 2018;Zhang, R. et al, 2018;, was also verified highly expressed in both CCA tissues and cells.…”