Long non-coding RNA PTENP1 inhibits proliferation and migration of breast cancer cells via AKT and MAPK signaling pathways

Abstract

We aimed to investigate the influence of long non-coding RNA (lncRNA) PTEN pseudogene-1 (PTENP1) on the proliferation, migration and cycle of breast cancer cells and its mechanism. Lentiviral vectors expressing PTENP1 were synthesized and breast cancer cells MCF7 were transfected with LV003-GFP-PTENP1 and LV003-GFP, respectively. The proliferation capacities of breast cancer cells were detected using CCK-8 assay, and the migration capacities of breast cancer cells were detected using scratch assay; flow cytometry was used to detect the cell cycles and Western blot was used to detect the expression levels of cyclin A2, CDK2, p-p44/42 MAPK, t-p44/42 MAPK, p-p38 MAPK, t-p38 MAPK, p-AKT, t-AKT in AKT and MAPK pathways. The absorbance values (A450) of cells in experimental group at 48 and 72 h were 1.4±0.3 and 2.3±0.47, respectively, which were significantly lower than those in control group (3.2±0.39, 3.4±0.58) (P<0.05). The number of cell colonies in experimental group was (48±13), which was significantly lower than that in control group (159±16) (P<0.01). The cell migration rate in experimental group was 22.8±3.3%, which was significantly lower than that in control group 61.8±5.2% (P<0.01). Western blot detection showed that the expression levels of cyclin A2, CDK2, p-AKT, p-p44/42 MAPK and p-p38 MAPK in experimental group were significantly decreased compared with those in control group. LncRNA PTENP1 can inhibit the proliferation and migration of breast cancer cells via the AKT and MAPK signaling pathways.

Pseudogene-Derived lncRNAs and Their miRNA Sponging Mechanism in Human Cancer

“…PTENP1 was reported as a tumor suppressor in development and progression of breast cancer. For instance, a recent study conducted by Tang et al (2017a) showed that PTENP1 suppressed proliferation and migration of breast cancer by inhibiting AKT and MAPK signaling pathways; another study performed by Gao et al (2019) demonstrated that PETNP1 increased expression of PTEN, thus mediating proliferation, invasion and drug resistance of breast cancer by activation of PI3K/AKT pathway. Connnexin 43 pseudogene, PsiCx43, increased sensitivity to cytotoxic chemotherapy of breast cancer (Bier et al, 2009).…”

Long Non-coding RNAs as Regulators of the Mitogen-activated Protein Kinase (MAPK) Pathway in Cancer

“…Its over-expression in MCF7 breast cancer cells has resulted in inhibition of cell proliferation and migration as well as down-regulation of cyclin A2, CDK2, p-AKT, p-p44/ 42 MAPK and p-p38 MAPK, so this lncRNA can impede the prol iferation and migration of breast cancer cells through modulation of AKT and MAPK signal ing pathways [64].…”

Down-regulation of lncRNA UCA1 enhances radiosensitivity in prostate cancer by suppressing EIF4G1 expression via sponging miR-331-3p

“…Thus, it is urgent to explore the novel therapeutic strategy to enhance tumor radiosensitivity for the treatment of PCa patients. Long non-coding RNAs (LncRNAs), as crucial modulators, participate in the initiation and development of many diseases, including cancers [3][4][5]. Urothelial cancer associated-1 (UCA1) was identified as an oncogene in various cancers [6][7][8].…”

“…Long non-coding RNAs (LncRNAs), as crucial modulators, participate in the initiation and development of many diseases, including cancers [ 3 – 5 ]. Urothelial cancer associated-1 (UCA1) was identified as an oncogene in various cancers [ 6 – 8 ].…”