Long non-coding RNA PTENP1 functions as a ceRNA to modulate PTEN level by decoying miR-106b and miR-93 in gastric cancer

Zhang, Rupeng; Guo, Yuenan; Ma, Zhenchi; Ma, Gang; Qin, Xue; Li, Fangxuan; Liu, Liren

doi:10.18632/oncotarget.15317

Cited by 107 publications

(89 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…31 These previous studies indicated the promotive effect of miR-17-5p and the suppressive effect of PTEN on GC, which was in line with our results. 20 Our results indicated a similar mechanism that HOTAIRM1 elevated PTEN expression through binding miR-17-5p. 32 Furthermore, some lncRNAs were supposed to act as competing endogenous RNAs to mediate important genes in human cancer.…”

supporting

confidence: 70%

“…17 PTEN loss was associated with the poor response to trastuzumab treatment of GC. 20 Considering the crucial functions of miR-15-7p and PTEN in GC, they were studied in our research to investigate their connections with differentially expressed lncRNA in GC. 19 It was also suggested to be modulated by lncRNA PTENP1 which sponged miR-106b and miR-93 in GC.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Long noncoding RNA HOTAIRM1 inhibits cell progression by regulating miR‐17‐5p/PTEN axis in gastric cancer

Zhao

Yang

et al. 2018

J of Cellular Biochemistry

View full text Add to dashboard Cite

Objectives This study was conducted to identify the significantly altered long noncoding RNAs (lncRNAs), messenger RNA (mRNA) and pathways in gastric cancer (GC). Methods We used microarray analysis to identify differentially expressed lncRNAs and mRNAs, whereas the obviously changed pathways were found by gene set enrichment analysis. The coexpression network of lncRNA and mRNA was constructed by Cytoscape, and their target relationships with miRNAs were predicted by miRcode and TargetScan. qRT‐PCR and Western blot were performed to determine the expression levels of mRNAs and proteins in tissues and cell lines. Dual‐luciferase reporter assay was applied to achieve the determination of the specific target relationships. Cell viability, migration, and apoptosis were detected by MTT assay, wound healing assay and flow cytometry, respectively. Through the xenograft assay, the gastric tumor was implanted into nude mice to investigate the influence of HOTAIRM1 in vivo. Results HOTAIRM1 and phosphatase and tensin homolog (PTEN) were both downregulated in GC, whereas miR‐17‐5p was upregulated. Moreover, the PI3K/AKT pathway was found activated in GC. HOTAIRM1 targeted miR‐17‐5p, whereas PTEN was the downstream target gene of miR‐17‐5p. HOTAIRM1 suppressed proliferation and migration of GC cell line and induced their apoptosis, whereas miR‐17‐5p played the opposite role on GC cell line. HOTAIRM1 also postponed tumor growth in vivo and inhibited the PI3K/AKT pathway in GC. Conclusions LncRNA HORAIRM1 suppressed the PI3K/AKT pathway in GC and inhibited the progression of GC by serving as a competing endogenous RNA of miR‐17‐5p, mediating the expression of PTEN.

show abstract

supporting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA HOTAIRM1 inhibits cell progression by regulating miR‐17‐5p/PTEN axis in gastric cancer

Zhao

Yang

et al. 2018

J of Cellular Biochemistry

View full text Add to dashboard Cite

show abstract

“…Currently, the lncRNA-miRNA-mRNA regulatory network is widely recognized; in this network, lncRNA acts as a natural miRNA sponge (ceRNA) to sponge miRNAs, thus decreasing the expression of miRNA targets and affecting posttranscriptional regulation [32]. For example, it is reported that lncRNA PTENP1 functioned as a ceRNA to modulate PTEN levels by decoying miR-106b and miR-93 in gastric cancer [33]. Similarly, Wang et al found that lncRNA TUG1 promotes migration and invasion by acting as a ceRNA of miR-335-5p in osteosarcoma cells [34].…”

Section: Cellular Physiology and Biochemistrymentioning

confidence: 99%

LncRNA HOTAIR Regulates CCND1 and CCND2 Expression by Sponging miR-206 in Ovarian Cancer

Chang¹,

Guo²,

Zhang³

et al. 2018

Cell Physiol Biochem

100

View full text Add to dashboard Cite

Background/Aims: The long noncoding RNA homeobox (HOX) transcript antisense intergenic RNA (HOTAIR) has been demonstrated to be a vital modulator in the proliferation and metastasis of ovarian cancer cells, but its potential molecular mechanism remains to be elucidated. In the current study, we aimed to uncover the biological role of lncRNA HOTAIR and its underlying regulatory mechanism in the progression and metastasis of ovarian cancer. Methods: HOTAIR expression was detected by quantitative RT-PCR (qRT-PCR) and northern blotting. The SKOV3 ovarian cancer cell line was chosen for the subsequent assays. In addition, the molecular mRNA and protein expression levels were examined by qRT-PCR and western blotting. The competitive endogenous RNA (ceRNA) mechanism was validated by bioinformatics analysis and a dual luciferase reporter gene assay. Results: HOTAIR expression was significantly higher in ovarian carcinoma tissues and cell lines than in the control counterparts. Both CCND1 and CCND2 were downstream targets of miR-206. The inhibition of HOTAIR elevated the expression of miR-206 and inhibited the expression of CCND1 and CCND2. Moreover, CCND1 and CCND2 were highly expressed in ovarian cancer tissues, and their expression was positively correlated with HOTAIR expression. Finally, the functional assays indicated that the anticancer effects of miR-206 could be rescued by the simultaneous overexpression of either CCND1 or CCND2 in ovarian cancer. Conclusion: HOTAIR enhanced CCND1 and CCND2 expression by negatively modulating miR-206 expression and stimulating the proliferation, cell cycle progression, migration and invasion of ovarian cancer cells.

show abstract

“…In gastric cancer, PTENP1 acts as a competing endogenous decoying miR‐106b and miR‐93, resulting in PTEN high expression . In addition, down‐regulation of PTENP1 promotes malignant behaviors in head and neck squamous cell carcinoma …”

Section: Lncrnas In Hallmarks Of Cancermentioning

confidence: 99%

“…57,58 In gastric cancer, PTENP1 acts as a competing endogenous decoying miR-106b and miR-93, resulting in PTEN high expression. 59 In addition, down-regulation of PTENP1 promotes malignant behaviors in head and neck squamous cell carcinoma. 60 The lncRNA HEIH is highly expressed in hepatitis B virus-related hepatocellular carcinoma and plays an important role in G0/G1 arrest.…”

Section: Evading Growth Suppressorsmentioning

confidence: 99%

Long non‐coding RNAs in cancer: Another layer of complexity

Oliveira

Mathias

et al. 2019

The Journal of Gene Medicine

View full text Add to dashboard Cite

We review the most well characterized long non‐coding RNAs (lncRNAs) with important roles in hallmarks of cancer, additionally including lncRNAs with a higher potential for clinical application. LncRNAs are transcripts larger than 200 nucleotides in length that do not appear to have protein‐coding potential, although some of those may produce small functional peptides. These transcripts have attracted significant attention from researchers as a result of their role in genetic regulation, including epigenetic, transcriptional and post‐transcriptional regulation, being involved in numerous biological processes, as well as being associated with multifactorial diseases, including tumorigenesis. The hallmarks of cancer include sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis and activating invasion/metastasis. Additionally, genome instability, inflammation, reprogramming of energy metabolism and evading immune destruction and lncRNAs are implicated in all hallmarks of cancer. Based on the great number of studies describing lncRNAs associated with diverse aspects of most tumor types, lncRNAs have essential roles in potentially all biological features of cancer cells and show great utility as diagnostic and prognostic markers, as exemplified by PCA3 lncRNA detection in prostate cancer diagnosis.

show abstract

Long non-coding RNA PTENP1 functions as a ceRNA to modulate PTEN level by decoying miR-106b and miR-93 in gastric cancer

Cited by 107 publications

References 29 publications

Long noncoding RNA HOTAIRM1 inhibits cell progression by regulating miR‐17‐5p/PTEN axis in gastric cancer

Long noncoding RNA HOTAIRM1 inhibits cell progression by regulating miR‐17‐5p/PTEN axis in gastric cancer

LncRNA HOTAIR Regulates CCND1 and CCND2 Expression by Sponging miR-206 in Ovarian Cancer

Long non‐coding RNAs in cancer: Another layer of complexity

Contact Info

Product

Resources

About