Long non‑coding RNA profiles in plasma exosomes of patients with gastric high‑grade intraepithelial neoplasia

Abstract

Long non-coding (lnc) RNAs in circulating exosomes are a new class of promising cancer biomarkers; however, their expression in exosomes derived from gastric high-grade intraepithelial neoplasia (GHGIN) has not been reported. In the present study, differentially expressed (DE) lncRNAs were analyzed in the peripheral blood collected from 5 patients with GHGIN and 5 healthy donors using high-throughput sequencing. Reverse transcription-quantitative PCR analysis was performed on 6 randomly selected DE lncRNAs to validate the reliability of the sequencing results. The potential roles of the DE lncRNAs in GHGIN were investigated using Gene Ontology (GO) and Kyoto Encyclopedia of Gene and Genome (KEGG) pathway enrichment analyses. A total of 25,145 lncRNAs were identified in all the samples and 83 DE lncRNAs were further screened, including 76 upregulated and 7 downregulated DE lncRNAs. GO and KEGG analyses predicted that the DE lncRNAs played notable roles in 'protein/macromolecule glycosylation', 'regulation of protein ubiquitination', 'renin-angiotensin system' and 'MAPK signaling pathways'. A lncRNA-micro (mi)RNA-mRNA interaction network was constructed and used to perform association analyses. It was found that 83 lncRNAs were abnormally expressed in GHGIN, with some potential functions associated with gastric cancer. Furthermore, the lncRNA-miRNA-mRNA interaction network indicated that 7 DE lncRNAs may play a notable role in the occurrence and development of GHGIN. The results of the present study showed the expression profiles of lncRNAs in human GHGIN, elucidated some of the molecular changes associated with GHGIN and improved the understanding of the molecular mechanisms underlying GHGIN and gastric cancer.

CircMTDH promotes the development of gastric cancer and the mechanism

“…Based on the exo and the results of high throughput RNA sequencing obtained in our previous study [29],…”

“…RNA isolation from exosomes was performed using Trizol (Life Technologies, USA), and the expression patterns of the target genes were determined via high-throughput RNA sequencing and bioinformatics analysis as outlined in our previous study [29]. The differential genes were con rmed in GC patients, exo, and GC cells by using the RT-qPCR method.…”

“…Following the endorsement from the Ethics Committee of the First Hospital of Jilin University, peripheral blood samples were obtained from both GC patients and healthy controls at the Department of Biobank, Division of Clinical Research, The First Hospital of Jilin University. Exo that was stored at -80℃ was extracted in our previous study [29]. The GC cell lines, including MKN-45, HGC-27, and AGS, along with human gastric mucosal epithelial cells (GES-1), were acquired from Shanghai Zhongqiao Xinzhou Biotechnology Co., Ltd.…”