Long non-coding RNA OIP5-AS1 regulates smoke-related chronic obstructive pulmonary disease via targeting micro RNA -410-3p/IL-13

Abstract

This investigation aimed to assess the levels of serum OIP5-AS1 and micro RNA-410-3p (miR-410-3p) in patients with chronic obstructive pulmonary disease (COPD) and their potential molecular mechanism. The levels of OIP5-AS1 and miR-410-3p as well as mRNA levels of IL-13 were measured. Pearson variable linear test was applied to analyze the correlations between forced expiratory volume in 1 second (FEV1) and OIP5-AS1. The receiver operating characteristic curve was used to predict the predictive possibility of OIP5-AS1. The viable cells were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry was used to detect the cell apoptosis. An enzyme-linked immunosorbent assay was performed to indicate the inflammatory situation of 16HBE cells. Luciferase activity assay was conducted to examine the relationships between OIP5-AS1 and miR-410-3p together with miR-410-3p and IL-13. Augmented levels of OIP5-AS1, declined levels of miR-410-3p, and enhanced expression of IL-13 were unveiled. The expression of OIP5-AS1 and miR-410-3p was related to the ratio of FEV1 respectively. OIP5-AS1 might serve as a diagnostic biomarker. Interference of OIP5-AS1 restored the abnormal cell viability, apoptosis, and inflammation in cigarette smoke extract (CSE)-stimulated 16HBE cells by regulating miR-410-3p and IL-13. OIP5-AS1 appeared to be a biomarker for distinguishing COPD patients from smokers. OIP5-AS1/miR-410-3p/IL-13 exerted function on the cell viability, apoptosis, and inflammation in CSE-steered cell models.

Identification and Validation of CDKN1A and HDAC1 as Senescence-Related Hub Genes in Chronic Obstructive Pulmonary Disease

“… 43 OIP5-AS1 is a diagnostic marker for COPD, and the OIP5-AS1/miR-410-3p/IL-13 axis plays a crucial role in regulating COPD. 13 Hence, we predicted that the HDAC1-related ceRNA might contribute to clarifying the effect of HDAC1 on senescence and the role of senescence in the development of COPD. The specific role and mechanism of ceRNA in COPD need to be further verified.…”

“…The expression of lncRNA OIP5-AS1 is elevated in CSE-stimulated 16HBE cells and can enhance the inflammation and cell apoptosis by modulating the miR-410-3p/IL-13 signaling. 13 LncRNA GAS5 functions as a ceRNA and regulates the miR-223-3p/NLRP3 axis to promote cell pyroptosis in COPD. 14 However, the role of SRGs-based ceRNA in COPD has not been investigated.…”

Dexmedetomidine promotes cell proliferation and inhibits cell apoptosis by regulating LINC00982 and activating the phosphoinositide-3-kinase (PI3K)/protein kinase B (AKT) signaling in hypoxia/reoxygenation-induced H9c2 cells

“…H9c2 cells were seeded into 96-well plate and treated with 10 µl MTT solution for 24 h. Then, 100 µl DMSO (Sigma-Aldrich) was added to each well and the plates were gently agitated for 10 min. The absorbance was determined at 490 nm using a microplate reader (BioTek) [ 14 ].…”

The Novel Regulatory Role of the lncRNA–miRNA–mRNA Axis in Chronic Inflammatory Airway Diseases

“…Hao and his colleagues ( Hao et al, 2021 ) revealed that the levels of opa interacting protein 5-antisense RNA 1 (OIP5-AS1) were elevated in smokers and smokers with COPD, and there was a negative connection between OIP5-AS1 and FEV 1 . Furthermore, OIP5-AS1 showed a talent in differentiating smokers with COPD from smokers, as revealed by the area under the curve (AUC) of 0.903.…”

“…Thirteen lncRNAs are implicated in the physio-pathological process of COPD by sponging miRNAs ( Figure 2 ). Of these, eleven differentially expressed lncRNAs have been found in human bio-samples (including whole blood, serum, sputum, and lung tissue samples) from COPD patients: NNT-AS1 ( Mei et al, 2020 ), MEG3 ( Lei et al, 2021 ), LUCAT1 ( Zhao et al, 2021 ), SNHG5 ( Shen et al, 2020 ), CASC2 ( Liu et al, 2021a ), TUG1 ( Gu et al, 2019 ), OIP5-AS1 ( Hao et al, 2021 ), LOC729178 ( Wang et al, 2021 ), XIST ( Chen et al, 2021 ), LINC00612 ( Luo et al, 2020 ), and MIR155HG ( Song et al, 2020 ). There is a possibility that these lncRNAs could be clinically helpful in diagnosing and treating COPD.…”