Long Non-Coding RNA OIP5-AS1 Inhibits the Proliferation and Migration of Esophageal Squamous Carcinoma Cells by Targeting FOXD1/miR-30a-5p Axis and the Effect of Micro- and Nano-Particles on Targeting Transfection System

Abstract

Esophageal cancer is one of the most common human malignancies and ranks sixth for global mortality; the major histological type is esophageal squamous cell carcinoma (ESCC). Here we assessed the effect of long non-coding (lnc) RNA OIP5-AS1 on the miR-30a-5p/Forkhead box protein D1 (FOXD1) axis in ESCC and investigated the underlying mechanism involving the ERK1/2 signaling pathway. lnc RNA OIP5-AS1 was highly expressed in human ESCC tissues and cells, targeted miR-30a-5p, and inhibited miR-30a-5p expression. Additionally, in human ESCC tissues, miR-30a-5p was poorly expressed, whereas FOXD1 mRNA and protein were highly expressed, with a negative correlation between miR-30a-5p and FOXD1 expression. miR-30a-5p targeted and inhibited FOXD1 expression. FOXD1 promoted the proliferation and invasion of ESCC and was related to the ERK1/2 signaling pathway; ERK1/2 inhibitors (LY-3214996) reversed the biological function of FOXD1. miR-30a-5p combined with FOXD1 regulated ERK1/2 expression and inhibited tumor growth in vivo. In this study, micro- and nano-particles were used as carriers to construct Nanocapsules carrying miR-30a-5p mimics and miR-30a-5p inhibitor through self-assembly method, so as to realize an efficient Nanocapsules delivery system of miR-30a-5p to esophageal cancer cells. It provides insights into targeted drug therapy and the development of micro- and nano-particles carriers.

LncRNA OIP5-AS1 Knockdown Facilitated the Ferroptosis and Immune Evasion by Modulating the GPX4 in Oesophageal Carcinoma

“…However, there is considerable evidence that GPX4 can be used as a reference marker for determining cellular ferroptosis [27]. Inactivation of GPX4 protein, which has the function of scavenging lipid peroxides, leads to disruption of oxidative homeostasis and disruption of membrane structure by lipid peroxides, triggering ferroptosis [28]. Fe 2+ , MDA, and lipid ROS as key markers of ferroptosis [29].…”

Decoding common genetic alterations between Barrett's esophagus and esophageal adenocarcinoma: A bioinformatics analysis

“…The role of lncRNA OIP5-AS1 , encoded by the anti-sense of the OIP5 gene, in promoting the EMT process, migration, proliferation, and invasion of tumor cells was elucidated in diverse cancers [ 62 , 63 ]. Consistently, recent investigations illustrated that lncRNA OIP5-AS1 could enhance the proliferation, migration, and invasion of esophageal cancer cells by sponging miR-30a [ 64 , 65 ].…”

Activating transcription factor 3 is a new biomarker correlation with renal clear cell carcinoma progression

“…As such, PAXIP1-AS2 was considered to be a TLS regulator. 39 Regarding, OIP5-AS1, this lncRNA reportedly functions as an oncogene in multiple malignancies, 40 – 42 but it also plays a protective role in esophageal squamous carcinoma cells 43 and esophageal carcinoma. 44 Taken together, the PAXIP1-AS2 and OIP5-AS1/hsa-miR-221-3p/ATF3 axis were identified as potential regulatory pathways in ccRCC.…”