Long non‑coding RNA OIP5‑AS1 facilitates the progression of ovarian cancer via the miR‑128‑3p/CCNG1 axis

Abstract

long non-coding rna (lncrna) o-phthalaldehydeinteracting protein 5 antisense transcript 1 (oiP5-aS1) serves major roles in the progression of various types of cancer. The present study investigated its biological function in ovarian cancer (oc) and its mechanisms. The levels of oiP5-aS1, microrna-128-3p (mir-128-3p) and cyclin G1 (ccnG1) were examined by reverse transcription-quantitative Pcr. cell viability, apoptosis, migration and invasion were detected to analyze cellular progression. Glycolytic metabolism was assessed by detecting the levels of glucose consumption and lactate production. ccnG1 and hexokinase 2 protein levels were measured by western blotting. dual-luciferase reporter assay, rna immunoprecipitation and rna pull-down assays were performed to affirm the interaction between two molecules. oiP5-aS1 was found to be upregulated in oc tissues and cells. Knockdown of oiP5-aS1 suppressed cell viability, migration, invasion and glycolysis while promoting apoptosis in oc cells. oiP5-aS1 interacted with mir-128-3p and functioned as an oncogene by sequestering mir-128-3p. in addition, ccnG1 was a target gene for mir-128-3p and mir-128-3p regulated the ccnG1-induced effects on oc cells by downregulating ccnG1. oiP5-aS1 upregulated the expression of ccnG1 via targeting mir-128-3p. oiP5-aS1 knockdown also inhibited tumor growth of oc in vivo by modulating the expression of mir-128-3p and ccnG1. collectively, these data illustrated that the oncogenic role of oiP5-aS1 in oc was associated with the mir-128-3p/ccnG1 axis at least in part. oiP5-aS1 might be a probable diagnostic and therapeutic biomarker for the treatment of oc patients.

lncRNA MSC-AS1 Aggravates Diabetic Nephropathy by Regulating the miR-325/CCNG1 Axis

“…CCNG1 has been reported to participate in the pathogenesis of human diseases by mediating lncRNA or miRNA. For example, lncRNA OIP5-AS1 promoted the progression of ovarian cancer by regulating CCNG1 [ 11 ]. MiR-122-5p inhibited cell proliferation, migration, and invasion by targeting CCNG1 in pancreatic ductal adenocarcinoma [ 12 ].…”

DPP10-AS1–Mediated Downregulation of MicroRNA-324-3p Is Conducive to the Malignancy of Pancreatic Cancer by Enhancing CLDN3 Expression

“…33 More importantly, miR-324-3p in ovarian cancer also confers an antitumor effect, and its effect was abolished when lncRNA-OIP5-AS1 acts as a sponge through competitive binding. 34 Moreover, our study furthered the mechanistic understanding and delineated that DPP10-AS1 competitively bound to miR-324-3p and thus upregulated the expression of tumor-supporting CLDN3. The migration distance and invasion ability of PC cells were diminished after the silencing of CLDN3.…”

Long non-coding RNA OIP5-AS1 suppresses microRNA-92a to augment proliferation and metastasis of ovarian cancer cells through upregulating ITGA6

“…Currently, OIP5-AS1 has been determined to overexpress in epithelial OC and depletion of OIP5-AS1 contributes to limit cellular aggressiveness and EMT process [28]. Moreover, Q-Y Liu et al have defined OIP5-AS1 with oncogenic property in OC and found that OIP5-AS1 knockdown represses OC cell viability, invasion, and migration [29]. A recent publication has uncovered that miR-92a-3p suppressed cell growth in Wilms' tumor [30].…”