Long non‑coding RNA MEG3 inhibits cell migration and invasion of non‑small cell lung cancer cells by regulating the miR‑21‑5p/PTEN axis

Lv, Dongjin; Bi, Qing; Li, Yunxia; Deng, Jiehui; Wu, Na; Shu, Hao; Zhao, Mingli

doi:10.3892/mmr.2021.11830

Cited by 25 publications

(13 citation statements)

References 41 publications

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“…In this study, bioinformatics analysis predicted that miR-21-5p was a potential target of NBTA1 and validated by the luciferase activity reporter assay. As reported previously, miR-21-5p inhibits non-small-cell lung cancer progression, colon adenocarcinoma, and thyroid papillary carcinoma [26,40,41]. In this study, it was confirmed through cell experiments that NBTA1 was targeted to bind to miR-21-5p and overexpression of miR-21-5p not only promoted the proliferation and invasion of EC and inhibited apoptosis but also significantly blocked the effect of NBTA1 on EC cells.…”

Section: Discussionsupporting

confidence: 87%

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lncRNA NBAT1 Inhibits Cell Metastasis and Promotes Apoptosis in Endometrial Cancer by Sponging miR-21-5p to Regulate PTEN

Tian

et al. 2022

Computational and Mathematical Methods in Medicine

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Objective. Long noncoding RNA neuroblastoma-associated transcript 1 (NBAT1) is implicated in the progression of various cancers. Nevertheless, its biological function in endometrial cancer (EC) remains unknown. Methods. The levels of NBAT1, miR-21-5p, and PTEN in EC cells and EC tissues were examined by RT-qPCR. Western blot was carried out to assess the protein expression of PTEN. The dual-luciferase reporter assay was conducted to explore the interactions among NBAT1, miR-21-5p, and PTEN. The effect of NBAT1 on EC proliferation, metastasis, and apoptosis was evaluated by CCK-8, transwell assays, wound healing, and flow cytometry. miR-21-5p mimics or NBAT1+miR-21-5p were transfected into HEC-1A and Ishikawa cells to investigate whether NBAT1 regulated EC tumorigenesis via sponging miR-21-5p. Results. NBAT1 is downregulated, and miR-21-5p is upregulated in EC cells and tumor tissues. Overexpression of NBAT1 inhibits the proliferation, migration, and invasion abilities of EC cells and facilitated apoptosis. NBAT1 directly binds and negatively regulates miR-21-5p in EC. miR-21-5p mimics reverses the effect of lncRNA NBAT1 overexpression on the proliferation and migration of EC cells. PTEN is a downstream gene of miR-21-5p. lncRNA NBTA1 elevates PTEN expression via sponging miR-21-5p. Conclusions. lncRNA NBAT1 acts as a tumor suppressor in EC via regulating PTEN through sponging miR-21-5p.

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Section: Discussionsupporting

confidence: 87%

“…Prior research has exposed that PTEN was a potential target of miR-21-5p [ 26 ]. The present study proved the binding site between miR-21-5p and PTEN ( Figure 5(a) ).…”

Section: Resultsmentioning

confidence: 99%

lncRNA NBAT1 Inhibits Cell Metastasis and Promotes Apoptosis in Endometrial Cancer by Sponging miR-21-5p to Regulate PTEN

Tian

et al. 2022

Computational and Mathematical Methods in Medicine

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“…PTEN is a well-known tumor suppressor gene that regulates Akt signaling to promote tumorigenesis and metastasis [ 15 , 16 , 17 ], including in RCC [ 41 ]. It has been reported to be a direct target of miR-21-5p in some malignancies [ 42 , 43 ]. Here, we assessed if PTEN could be the target of miR-21-5p driving RCC aggression.…”

Section: Resultsmentioning

confidence: 99%

The miRNA-21-5p Payload in Exosomes from M2 Macrophages Drives Tumor Cell Aggression via PTEN/Akt Signaling in Renal Cell Carcinoma

Zhang

Ishihara

et al. 2022

IJMS

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M2 macrophages in the tumor microenvironment are important drivers of cancer metastasis. Exosomes play a critical role in the crosstalk between different cells by delivering microRNAs or other cargos. Whether exosomes derived from pro-tumorigenic M2 macrophages (M2-Exos) could modulate the metastatic behavior of renal cell carcinoma (RCC) is unclear. This study found that M2-Exos promotes migration and invasion in RCC cells. Inhibiting miR-21-5p in M2-Exos significantly reversed their pro-metastatic effects on RCC cells in vitro and in the avian embryo chorioallantoic membrane in vivo tumor model. We further found that the pro-metastatic mechanism of miR-21-5p in M2-Exos is by targeting PTEN-3′UTR to regulate PTEN/Akt signaling. Taken together, our results demonstrate that M2-Exos carries miR-21-5p promote metastatic features of RCC cells through PTEN/Akt signaling. Reversing this could serve as a novel approach to control RCC metastasis.

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“…For instance, CCDC144NL‐AS1, 23 AK027294, 24 and HOXA11‐AS 25 are overexpressed in NSCLC and exacerbate malignancy. In contrast, downregulation of ZNF674‐AS1, 26 WT1‐AS, 27 and MEG3 28 is identified in NSCLC and can lower the aggressive events of NSCLC. However, the functions of RNASEH1‐AS1 in NSCLC have rarely been explored.…”

Section: Discussionmentioning

confidence: 95%

Long noncoding RNASEH1‐AS1 exacerbates the progression of non‐small cell lung cancer by acting as a ceRNA to regulate microRNA‐516a‐5p/FOXK1 and thereby activating the Wnt/β‐catenin signaling pathway

Zhang

Huang

et al. 2022

Cancer Medicine

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Long non‑coding RNA MEG3 inhibits cell migration and invasion of non‑small cell lung cancer cells by regulating the miR‑21‑5p/PTEN axis

Cited by 25 publications

References 41 publications

lncRNA NBAT1 Inhibits Cell Metastasis and Promotes Apoptosis in Endometrial Cancer by Sponging miR-21-5p to Regulate PTEN

lncRNA NBAT1 Inhibits Cell Metastasis and Promotes Apoptosis in Endometrial Cancer by Sponging miR-21-5p to Regulate PTEN

The miRNA-21-5p Payload in Exosomes from M2 Macrophages Drives Tumor Cell Aggression via PTEN/Akt Signaling in Renal Cell Carcinoma

Long noncoding RNASEH1‐AS1 exacerbates the progression of non‐small cell lung cancer by acting as a ceRNA to regulate microRNA‐516a‐5p/FOXK1 and thereby activating the Wnt/β‐catenin signaling pathway

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