Long Non-Coding RNA LINC01929 Accelerates Progression of Oral Squamous Cell Carcinoma by Targeting the miR-137-3p/FOXC1 Axis

Abstract

Recently, additional long noncoding RNAs (lncRNAs) have been identified and their possible roles were investigated in a variety of human tumors. One of these lncRNAs, LINC01929, promoted the progression of some cancers, whereas its expression and biological function in human oral squamous cell carcinoma (OSCC) remains still mostly uncertain. The LINC01929 expression in OSCC tissues or cell lines was identified via quantitative real-time polymerase chain reaction. The cell counting kit-8, transwell migration, wound-healing, and flow cytometry assays were utilized to characterize the functions of LINC01929 in OSCC cells. The interactive relationships between LINC01929 and miR-137-3p, miR-137-3p and Forkhead box C1 (FOXC1) were investigated by the dual-luciferase activity assay. Our findings demonstrated that LINC01929 was highly expressed in OSCC tissue samples and cell lines, whereas miR-137-3p expression was downregulated. LINC01929 acted as a carcinogenic lncRNA with accelerated OSCC cell proliferation, migration and invasion, and suppression of apoptosis. We further indicated that LINC01929 facilitated tumor growth in xenograft mouse models. Mechanistically, LINC01929 acted as a sponge for miR-137-3p to elevate FOXC1 expression, which is the target of miR-137-3p. In addition, downregulated miR-137-3p expression rescued the suppressive behaviors of LINC01929 knockdown on the biological behaviors of OSCC cells. Taken together, LINC01929 functioned as a tumor-promoting lncRNA via the miR-137-3p/FOXC1 axis in OSCC, suggesting novel targets for OSCC therapy.

Hypoxia-Related Long Non-Coding RNA Signature Predicts the Prognosis of Breast Cancer

“…High LINC01614 expression was also markedly associated with the epithelial-mesenchymal transition (EMT), in uencing metastasis and the prognosis of several cancers 56 .LncRNA LINC02446 suppresses the proliferation and metastasis of bladder cancer cells by binding with EIF3G and regulating the mTORsignaling pathway 57 . Results from a related study suggest that TFAP2A-AS1 acts as a tumour suppressor in BC via the miR-933/SMAD2 axis 58 .lncLINC01929 accelerates the progression of oral squamous cell carcinoma by targeting the miR-137-3p/FOXC1 axis and is associated with survival in the TCGA dataset [59][60][61] . The high expression of KLHDC7B-DT aggravates pancreatic ductal adenocarcinoma development via inducing cross-talk between cancer cells and macrophages 62 .…”

The LINC01929/miR-6875-5p/ADAMTS12 Axis in the ceRNA Network Regulates the Development of Advanced Bladder Cancer

“…Overexpression of LINC01929 and ADAMTS12 enhanced cell invasion, while overexpression of miR-6785-5p had the opposite effect. lncRNA function may depend on its downstream binding molecules, such as ceRNA, which can regulate changes in target mRNA expression by competitively binding miRNA ( 50 ). In gastric cancer, lncRNA MT1JP binds miR-92a-3p to regulate FBXW7 expression, which in turn affects gastric cancer progression ( 51 ).…”

Identifying glycolysis-related LncRNAs for predicting prognosis in breast cancer patients

“…So far, among 14 prognostic lncRNAs, the function AC002546.1, AC010503.4, AC092142.1, AC092718.4, AL031316.1, AL136084.3 and U62317.1 have not been previously explored in breast cancer or other cancers. LINC01929 has been found positively correlated with tumor progression in liver cancer, oral squamous cell carcinoma and lung cancer[ 23 , 24 , 25 ]. Che et al indicated that LINC01929 promotes tumor progression through miR-137-3p/FOXC1 axis in oral squamous cell carcinoma [ 23 ].…”