Long Non-coding RNA LINC01234 Regulates Proliferation, Invasion and Apoptosis in Esophageal Cancer Cells

Ghaffar, Maliha; Khodahemmati, Sara; Li, Jintao; Shahzad, Muhammad; Wang, Minglian; Wang, Yangjunqi; Li, Changshuo; Su, Chen; Zeng, Yi

doi:10.7150/jca.26095

Cited by 29 publications

(29 citation statements)

References 32 publications

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“…To this end, we knocked down LINC01234 using siRNA; lower LINC01234 expression dramatically decreased cell viability, but increased apoptosis rate and upregulated apoptosis-related proteins. Knockdown of LINC01234 has been reported to induce cell cycle arrest and promote apoptosis in gastric cancer cells [14] and esophageal cancer cells [15]. Furthermore, the levels of several apoptosis-related proteins, including caspase-3, PARP, BAX, and BCL2, were altered in LINC01234 knockdown SW480 and HCT116 cells; similar results have also been reported in gastric cancer cells [14].…”

Section: Discussionsupporting

confidence: 74%

“…LINC01234 has recently attracted attention because of its high expression levels in different tumors [10], such as triple-negative breast cancer [13], gastric cancer [14], esophageal cancer [15], and colorectal adenocarcinoma [12]. We analyzed a total of 136 patients at different tumor stages and tumor sizes and containing positive or negative regional lymph node invasion.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, LINC01234 has been suggested as a potential cancer biomarker owing to its positive correlation with tumor size, tumor stage, and metastasis, as well as a negative correlation with patient survival [12,14]. In vitro results have further demonstrated that LINC01234 promotes the proliferation, migration, and invasion and inhibits the apoptosis of esophageal cancer cells [15]. However, little is known regarding the underlying mechanism through which LINC01234 promotes CRC development.…”

Section: Introductionmentioning

confidence: 99%

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Upregulated Long Noncoding RNA LINC01234 Predicts Unfavorable Prognosis for Colorectal Cancer and Negatively Correlates With KLF6 Expression

Jiang

Zhu

et al. 2020

Ann Lab Med

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Background: LINC01234, a long noncoding RNA (lncRNA), is overexpressed in several cancers, including colorectal cancer (CRC). We investigated the role of LINC01234 in CRC development and confirmed its correlation with Krüppel-like factor 6 (KLF6), a tumor suppressor gene that is dysregulated in CRC. Methods:We tested mRNA levels using quantitative reverse transcription PCR (qRT-PCR). Tissue samples from patients with CRC, inflammatory bowel disease (IBD), hyperplastic polyp, and adenoma were included. Correlations between clinicopathological parameters, overall survival (OS) rate, and LINC01234 were analyzed using Kruskal-Wallis H test. Additionally, cell proliferation, apoptosis, and tumor formation in nude mice were tested to investigate the mechanism of LINC01234. Western blotting was used to determine protein levels.Results: LINC01234 expression was significantly upregulated in CRC tissues and CRC cell lines than in non-tumor tissues and normal epithelial cells, respectively. LINC01234 was associated with high tumor stage, larger tumor size, and metastasis. Patients with higher LINC01234 expression showed reduced OS. Cell proliferation was inhibited by LINC01234 knockdown, whereas apoptosis was enhanced. Mice injected with SW480 cells with LINC01234 knockdown displayed decreased tumor volume, weight, and Ki-67 levels compared with those injected with control cells. KLF6 was negatively regulated by LINC01234. Overexpression of KLF6 showed effects similar to those observed following LINC01234 knockdown on cell proliferation and apoptosis.Conclusions: LINC01234 could be a prognostic biomarker in CRC patients. Upregulation of LINC01234 in CRC promotes tumor development through negative regulation of KLF6. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Upregulated Long Noncoding RNA LINC01234 Predicts Unfavorable Prognosis for Colorectal Cancer and Negatively Correlates With KLF6 Expression

Jiang

Zhu

et al. 2020

Ann Lab Med

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“…Using this method to screen lncRNA signature, we finally developed a novel RS (Rui et al, 2018). In addition, LINC01234 was a valuable tumor-associated lncRNA, the altered expression of which was observed in various malignancies, including colon, esophageal, gastric, and breast cancer (Chen et al, 2018;Ghaffar et al, 2018;Guo et al, 2016;Lin, Zhang, Chen, Bai, & Zhang, 2019). Further investigation demonstrated that the LINC01234-miR-204-5p-CBFB axis plays a critical role in gastric cancer tumorigenesis (Chen et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Prediction of head and neck squamous cell carcinoma survival based on the expression of 15 lncRNAs

Zhang

Wang

Guo

et al. 2019

Journal Cellular Physiology

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Recent evidence suggests that long noncoding RNAs (lncRNAs) are essential regulators of many cancer‐related processes, including cancer cell proliferation, invasion, and migration. There is thus a reason to believe that the detection of lncRNAs may be useful as a diagnostic and prognostic strategy for cancer detection, however, at present no effective genome‐wide tests are available for clinical use, constraining the use of such a strategy. In this study, we performed a comprehensive assessment of lncRNAs expressed in samples in the head and neck squamous cell carcinoma (HNSCC) cohort available in The Cancer Genome Atlas database. A risk score (RS) model was constructed based on the expression data of these 15 lncRNAs in the validation data set of HNSCC patients and was subsequently validated in validation data set and the entire data set. We were able to stratify patients into high‐ and low‐risk categories, using our lncRNA expression panel to determine an RS, with significant differences in overall survival (OS) between these two groups in our test set (median survival, 1.863 vs. 5.484 years; log‐rank test, p < 0.001). We were able to confirm the predictive value of our 15‐lncRNA signature using both a validation data set and a full data set, finding our signature to be reproducible and effective as a means of predicting HNSCC patient OS. Through the multivariate Cox regression and stratified analyses, we were further able to confirm that the predictive value of this RS was independent of other predictive factors such as clinicopathological parameters. The Gene set enrichment analysis revealed potential functional roles for these 15 lncRNAs in tumor progression. Our findings indicate that an RS established based on a panel of lncRNA expression signatures can effectively predict OS and facilitate patient stratification in HNSCC.

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“…Linc01234 (ENSG00000249550) is a conserved lncRNA located at 12q24.13 that is aberrantly expressed in several cancers [10][11][12]. Linc01234 was closely correlated with poor survival in OSCC and breast cancer through analyses of the TCGA database [13,14].…”

Section: Introductionmentioning

confidence: 99%

Linc01234 promotes cell proliferation and metastasis in oral squamous cell carcinoma via miR-433/PAK4 axis

Liu

Jian

et al. 2020

BMC Cancer

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Background: Increasing studies have demonstrated that long non-coding RNAs (lncRNAs) play an important role in tumor progression. However, the potential biological functions and clinical importance of Linc01234 in oral squamous cell carcinoma (OSCC) remain unclear. Methods: We evaluated the expression profile and prognostic value of Linc01234 in OSCC tissues by RT-qPCR. Then, functional in vitro experiments were performed to investigate the effects of Linc01234 on tumor growth, migration and invasion in OSCC. Mechanistically, RT-qPCR, bioinformatic analysis and dual luciferase reporter assays were performed to identify a competitive endogenous RNA (ceRNA) mechanism involving Linc01234, miR-433-3p and PAK4. Results: We found that Linc01234 was clearly upregulated in OSCC tissues and cell lines, and its level was positively associated with T stage, lymph node metastasis, differentiation and poor prognosis of patients with OSCC. Our results shown that Linc01234 inhibited cell proliferation and metastatic abilities in CAL27 and SCC25 cells following its knockdown. Mechanistic analysis indicated that Linc01234 may act as a ceRNA (competing endogenous RNA) of miR-433-3p to relieve the repressive effect of miR-433-3p on its target PAK4. Conclusions:Our results indicated that Linc01234 promotes OSCC progression through the Linc01234/miR-433/ PAK4 axis and might be a potential therapeutic target for OSCC.

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Long Non-coding RNA LINC01234 Regulates Proliferation, Invasion and Apoptosis in Esophageal Cancer Cells

Cited by 29 publications

References 32 publications

Upregulated Long Noncoding RNA LINC01234 Predicts Unfavorable Prognosis for Colorectal Cancer and Negatively Correlates With KLF6 Expression

Upregulated Long Noncoding RNA LINC01234 Predicts Unfavorable Prognosis for Colorectal Cancer and Negatively Correlates With KLF6 Expression

Prediction of head and neck squamous cell carcinoma survival based on the expression of 15 lncRNAs

Linc01234 promotes cell proliferation and metastasis in oral squamous cell carcinoma via miR-433/PAK4 axis

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