Long non‐coding RNA HCG11 modulates glioma progression through cooperating with miR‐496/CPEB3 axis

Abstract

Objectives It has been widely reported that long non‐coding RNAs (lncRNAs) can participate in multiple biological processes of human cancers. lncRNA HLA complex group 11 (HCG11) has been reported in human cancers as a tumour suppressor. This study focused on investigating the function and mechanism of HCG11 in glioma. Materials and methods Based on The Cancer Genome Atlas (TCGA) data set and qRT‐PCR analysis, the expression pattern of HCG11 was identified in glioma samples. The mechanism associated with HCG11 downregulation was determined by mechanism experiments. Gain‐of‐function assays were conducted for the identification of HCG11 function in glioma progression. Mechanism investigation based on the luciferase reporter assay, RIP assay and pull‐down assay was used to explore the downstream molecular mechanism of HCG11. The role of molecular pathway in the progression of glioma was analysed in accordance with the rescue assays. Results HCG11 was expressed at low level in glioma samples compared with normal samples. FOXP1 could bind with HCG11 and transcriptionally inactivated HCG11. Overexpression of HCG11 efficiently suppressed cell proliferation, induced cell cycle arrest and promoted cell apoptosis. HCG11 was predominantly enriched in the cytoplasm of glioma cells and acted as a competing endogenous RNAs (ceRNAs) by sponging micro‐496 to upregulate cytoplasmic polyadenylation element binding protein 3 (CPEB3). CEPB3 and miR‐496 involved in HCG11‐mediated glioma progression. Conclusions HCG11 inhibited glioma progression by regulating miR‐496/CPEB3 axis.

<p>Long Noncoding MAGI2-AS3 Suppresses Several Cellular Processes of Lung Squamous Cell Carcinoma Cells by Regulating miR-374a/b-5p/CADM2 Axis</p>

“…As examples, HCG11 inhibits cell glioma growth by modulating miR-496/CPEB or miR-4425/ MTA3 axis. 10,11 Up-regulated HEIH promotes colorectal cancer tumorigenesis by cooperating with miR-939 to repress the transcription of Bcl-xl. 12 Recently, MAGI2 antisense RNA 3 (MAGI2-AS3) is reported to act as a tumor suppressor in bladder cancer, breast cancer and hepatocellular carcinoma.…”

Identification and Validation of RNA&nbsp;Binding Protein-associated Prognostic Model for Neuroblastoma

“…CPEB3 can disrupt the crosstalk between cancer cells and tumor-associated macrophages through IL-6R/STAT3 signaling, thereby inhibiting epithelial-mesenchymal transition. Studies have found that CPEB3 is related to the tumorigenesis and development of glioma [25], high-grade serous ovarian cancer [26], colorectal cancer [27], hepatocellular carcinoma [28], cervical cancer [29].…”

Long non‐coding RNA HCG11 sponging miR‐522‐3p inhibits the tumorigenesis of non‐small cell lung cancer by upregulating SOCS5

“…14 LncRNA HCG11 has been found to inhibit glioma progression through cooperating with miR-496/CPEB3 axis. 15 These studies indicate that the function of lncRNA HCG11 is tissue specific. Moreover, the role of lncRNA HCG11 in NSCLC remains unclear.…”