Long non-coding RNA <i>Gm10561</i> promotes myogenesis by sponging miR-432

Wang, Shanshan; Tan, Baohua; Xiao, Liyao; Zeng, Jiekang; Zhao, Xinming; Hong, Linjun; Li, Zicong; Cai, Guangyan; Zheng, Enqin; Gu, Tao; Wu, Zhenfang

doi:10.1080/15592294.2022.2105052

Cited by 8 publications

(8 citation statements)

References 76 publications

(74 reference statements)

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“…MyHC is also concerned because it is a skeletal muscle-specific contractile protein expressed during muscle development [ 35 ]. On the other hand, accumulating evidence supports the fact that lncRNAs regulate target genes by competitively adsorbing miRNAs, which is termed the ceRNA hypothesis [ 16 , 36 , 37 , 38 ]. For instance, linc-MD1, a muscle-specific lncRNA, competes with miR-133 and miR-135, to upregulate the expression of myocyte enhancer factor 2C (MEF2C) and mastermind-like transcriptional coactivator 1 (MAML1), which activate muscle-specific gene expression and regulate myoblast differentiation in mouse and human [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals the Profile of Long Non-Coding RNAs during Myogenic Differentiation in Goats

Yang

Zhou

Xue

et al. 2023

IJMS

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The long non-coding RNAs (lncRNAs) are emerging as essential regulators of the growth and development of skeletal muscles. However, little is known about the expression profiles of lncRNAs during the proliferation and differentiation of skeletal muscle satellite cells (MuSCs) in goats. In this study, we investigate potential regulatory lncRNAs that govern muscle development by performing lncRNA expression profiling analysis during the proliferation (cultured in the growth medium, GM) and differentiation (cultured in the differentiation medium, DM1/DM5) of MuSCs. In total, 1001 lncRNAs were identified in MuSC samples, and 314 differentially expressed (DE) (FDR < 0.05, |log2FC| > 1) lncRNAs were screened by pairwise comparisons from three comparison groups (GM-vs-DM1, GM-vs-DM5, DM1-vs-DM5). Moreover, we identified the cis-, trans-, and antisense-regulatory target genes of DE lncRNAs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses showed that these target genes were significantly enriched in muscle development-related GO terms and KEGG pathways. In addition, the network of interactions between DE lncRNAs and their target genes was identified, which included well-known myogenesis regulators such as Myogenic differentiation 1 (MyoD), Myogenin (MyoG), and Myosin heavy chain (MyHC). Meanwhile, competing endogenous RNA (ceRNA) network analysis showed that 237 DE lncRNAs could bind to 329 microRNAs (miRNAs), while miRNAs could target 564 mRNAs. Together, our results provide a genome-wide resource of lncRNAs that may contribute to myogenic differentiation in goats and lay the groundwork for future investigation into their functions during skeletal muscle development.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals the Profile of Long Non-Coding RNAs during Myogenic Differentiation in Goats

Yang

Zhou

Xue

et al. 2023

IJMS

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“…In this study, E230016M11Rik and Gm20515 were found to be involved in muscle atrophy for the first time; however, the underlying mechanisms are unknown. A recent study showed the important role of lncRNA Gm10561 in myogenesis [ 45 ], which was upregulated during myogenic differentiation and is highly expressed in skeletal muscle. In that study, authors indicated the function of Gm10561 as a competing endogenous RNA (ceRNA) for miRNA miR-432 .…”

Section: Discussionmentioning

confidence: 99%

Up- and Downregulated Genes after Long-Term Muscle Atrophy Induced by Denervation in Mice Detected Using RNA-Seq

Sawano

Fukushima

Akasaka

et al. 2023

Life

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Skeletal muscle atrophy occurs rapidly as a result of inactivity. Although there are many reports on changes in gene expression during the early phase of muscle atrophy, the patterns of up-and downregulated gene expression after long-term and equilibrated muscle atrophy are poorly understood. In this study, we comprehensively examined the changes in gene expression in long-term denervated mouse muscles using RNA-Seq. The murine right sciatic nerve was denervated, and the mice were housed for five weeks. The cross-sectional areas of the hind limb muscles were measured using an X-ray CT system 35 days after denervation. After 28 d of denervation, the cross-sectional area of the muscle decreased to approximately 65% of that of the intact left muscle and reached a plateau. Gene expression in the soleus and extensor digitorum longus (EDL) muscles on the 36th day was analyzed using RNA-Seq and validated using RT-qPCR. RNA-Seq analysis revealed that three genes—Adora1, E230016M11Rik, and Gm10718—were upregulated and one gene—Gm20515—was downregulated in the soleus muscle; additionally, four genes—Adora1, E230016M11Rik, Pigh, and Gm15557—were upregulated and one gene—Fzd7—was downregulated in the EDL muscle (FDR < 0.05). Among these genes, E230016M11Rik, one of the long non-coding RNAs, was significantly upregulated in both the muscles. These findings indicate that E230016M11Rik could be a candidate gene for the maintenance of atrophied skeletal muscle size and an atrophic state.

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“…Primers for amplification of dfrB10 and pUC19 backbone (without AmpR, retaining the AmpR promoter) were designed using CE Design software (Vazyme, Nanjing, China). The Tks Gflex DNA Polymerase (Takara, Otsu, Japan) was used to amplify the pUC19 backbone and the dfrB10 fragment ( 31 ). The ClonExpress Ultra One Step Cloning Kit (Vazyme, Nanjing, China) was used to ligate fragments of dfrB10 and pUC19 backbone ( 32 ).…”

Section: Methodsmentioning

confidence: 99%

Construction and functional verification of size-reduced plasmids based on TMP resistance gene dfrB10

Li,

Hu,

Hua

et al. 2023

Microbiol Spectr

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Antibiotic resistance screening is crucial in the construction of recombinant plasmids. The dfrB10 gene, a short-size trimethoprim (TMP)-resistance type B dihydrofolate reductase gene, is a promising antibiotic drug screening marker for plasmid construction in molecular genetic research. Currently, available plasmid backbones (without the resistance gene) were amplified using PCR, and then we ligated these products with dfrB10 gene fragments via homologous recombination to construct new plasmid vectors with TMP resistance. The cloning, expression, and gene editing functions of the new plasmids based on their different purposes were verified. By successfully replacing the original resistance genes of pUC19, pET28a, pcDNA3.1, and pX330 plasmids with dfrB10 , we constructed a series of plasmids with TMP resistance. The change of the resistance selection marker to TMP did not affect the function of the plasmids compared to the original ones. Furthermore, a short (988 bp) TMP-resistant miniature tool plasmid, pTMi, was constructed based on the pUC19 plasmid. With the use of the dfrB10 gene as a resistance marker under TMP drug screening, various size-reduced tool plasmids can be routinely constructed. These plasmids have similar functions to the original ones and can be used for research applications in molecular genetics. IMPORTANCE Plasmid size is one of the factors affecting transfection efficacy in most of the molecular genetic research studies. One effective approach for reducing plasmid size is to replace relatively large, conventional antibiotic resistance genes with the short-size dfrB10 gene. The successful construct of a series of dfrB10- based tool plasmids and their functional validation, via comparison with original plasmids, suggest that dfrB10 is a potent drug resistance selection marker. The antibiotic trimethoprim offers convenient usage comparable to that of ampicillin or kanamycin. Additionally, fluorescence analysis has demonstrated the compatibility of TMP with protein expression in various host cells. Based on these findings, TMP- dfrB10 could be an alternative choice for future use in molecular genetic research studies that require miniature plasmids to achieve optimal results.

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Long non-coding RNA Gm10561 promotes myogenesis by sponging miR-432

Cited by 8 publications

References 76 publications

Transcriptome Analysis Reveals the Profile of Long Non-Coding RNAs during Myogenic Differentiation in Goats

Transcriptome Analysis Reveals the Profile of Long Non-Coding RNAs during Myogenic Differentiation in Goats

Up- and Downregulated Genes after Long-Term Muscle Atrophy Induced by Denervation in Mice Detected Using RNA-Seq

Construction and functional verification of size-reduced plasmids based on TMP resistance gene dfrB10

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