Long non-coding RNA H19 promotes corneal neovascularization by targeting microRNA-29c

Sun, Baoqi; Ding, Yiheng; Jin, Xin; Xu, Shuo; Zhang, Hong

doi:10.1042/bsr20182394

Cited by 20 publications

(12 citation statements)

References 42 publications

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“…This result seems to be consistent with the idea that lncRNA may be important in maintaining tissue identity [127]. The involvement of lncRNA H19 in neovascularization has recently described with lncRNA H19 acting as a molecular sponge for miR-29c to regulate the expression of VEGF-A [128].…”

Section: Non-coding Rnas In Lymphangiogenesissupporting

confidence: 90%

Role of Endogenous Regulators of Hem- And Lymphangiogenesis in Corneal Transplantation

Clahsen

Büttner

Hatami

et al. 2020

JCM

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Under normal conditions, the cornea, being the transparent "windscreen" of the eye, is free of both blood and lymphatic vessels. However, various diseases of the eye, like infections, can interfere with the balance between promoting and inhibiting factors, which leads to ingrowth of blood and lymphatic vessels. The newly formed lymphatic vessels increase the risk of graft rejection after subsequent corneal transplantation. Corneal transplantation is one of the most commonly performed transplantations worldwide, with more than 40,000 surgeries per year in Europe. To date, various anti-hem-and anti-lymphangiogenic treatment strategies have been developed specifically for the corneal vascular endothelial growth factor (VEGF) pathway. Currently, however, no treatment strategies are clinically available to specifically modulate lymphangiogenesis. In this review, we will give an overview about endogenous regulators of hem-and lymphangiogenesis and discuss potential new strategies for targeting pathological lymphangiogenesis. Furthermore, we will review recently identified modulators and demonstrate that the cornea is a suitable model for the identification of novel endogenous modulators of lymphangiogenesis. The identification of novel modulators of lymphangiogenesis and a better understanding of the signaling pathways involved will contribute to the development of new therapeutic targets for the treatment of pathological lymphangiogenesis. This, in turn, will improve graft rejection, not only for the cornea.

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Section: Non-coding Rnas In Lymphangiogenesissupporting

confidence: 90%

Role of Endogenous Regulators of Hem- And Lymphangiogenesis in Corneal Transplantation

Clahsen

Büttner

Hatami

et al. 2020

JCM

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“…In the present study, H19 was downregulated in the dermal fibroblast exposure to FGF2, suggesting FGF2 attenuated the expression of H19. Conversely, Sun et al reported the H19 levels were remarkably increased in FGF2-treated human umbilical vein endothelial cells (Sun et al 2019). Thus, our study indicated the FGF2-mediated H19 expression appeared to exhibit a context-dependent pattern.…”

Section: Discussioncontrasting

confidence: 51%

Peer Review #1 of "RNA sequencing analysis of FGF2-responsive transcriptome in skin fibroblasts (v0.1)"

2021

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Background: Fibroblast growth factor 2 (FGF2) is a highly pleiotropic cytokine with antifibrotic activity in wound healing. During the process of wound healing and fibrosis, fibroblasts are the key players. Although accumulating evidence has suggested the antagonistic effects of FGF2 in the activation process of fibroblasts, the mechanisms by which FGF2 hinders the fibroblast activation remains incompletely understood. This study aimed to identify the key genes and their regulatory networks in skin fibroblasts treated with FGF2. Methods: RNA-seq was performed to identify the differentially expressed mRNA (DEGs) and lncRNA between FGF2-treated fibroblasts and control. DEGs were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Furthermore, the networks between mRNAs and lncRNAs were constructed by Pearson correlation analysis and the networkanalyst website. Finally, hub genes were validated by real time-PCR. Results: Between FGF2-treated fibroblasts and control fibroblasts, a total of 1475 DEGs was obtained. These DEGs were mainly enriched in functions such as the ECM organization, cell adhesion, and cell migration. And they were mainly involved in ECMreceptor interaction, PI3K-Akt signaling, and the Hippo pathway. The hub DEGs included COL3A1, COL4A1, LOX, PDGFA, TGFBI, and ITGA10. Subsequent real-time PCR, as well as bioinformatics analysis consistently demonstrated that the expression of ITGA10 was significantly upregulated while the other 5 DEGs (COL3A1, COL4A1, LOX, PDGFA, TGFBI) were downregulated in FGF2-treated fibroblasts. Meanwhile, 213 differentially expressed lncRNAs were identified and three key lncRNAs (HOXA-AS2, H19, and SNHG8) were highlighted in FGF2-treated fibroblasts. Conclusion: The current study comprehensively analyzed the FGF2-responsive transcriptional profile and provided candidate mechanisms that may account for FGF2-mediated wound healing.

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“…In the present study, H19 was downregulated in the dermal fibroblast exposure to FGF2, suggesting FGF2 attenuated the expression of H19. Conversely, Sun et al (2019) reported the H19 levels were remarkably increased in FGF2-treated human umbilical vein endothelial cells. Thus, our study indicated the FGF2-mediated H19 expression appeared to exhibit a context-dependent pattern.…”

Section: Discussionmentioning

confidence: 96%

RNA sequencing analysis of FGF2-responsive transcriptome in skin fibroblasts

Tang

Zhou

et al. 2021

PeerJ

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Background Fibroblast growth factor 2 (FGF2) is a highly pleiotropic cytokine with antifibrotic activity in wound healing. During the process of wound healing and fibrosis, fibroblasts are the key players. Although accumulating evidence has suggested the antagonistic effects of FGF2 in the activation process of fibroblasts, the mechanisms by which FGF2 hinders the fibroblast activation remains incompletely understood. This study aimed to identify the key genes and their regulatory networks in skin fibroblasts treated with FGF2. Methods RNA-seq was performed to identify the differentially expressed mRNA (DEGs) and lncRNA between FGF2-treated fibroblasts and control. DEGs were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Furthermore, the networks between mRNAs and lncRNAs were constructed by Pearson correlation analysis and the networkanalyst website. Finally, hub genes were validated by real time-PCR. Results Between FGF2-treated fibroblasts and control fibroblasts, a total of 1475 DEGs was obtained. These DEGs were mainly enriched in functions such as the ECM organization, cell adhesion, and cell migration. They were mainly involved in ECM-receptor interaction, PI3K-Akt signaling, and the Hippo pathway. The hub DEGs included COL3A1, COL4A1, LOX, PDGFA, TGFBI, and ITGA10. Subsequent real-time PCR, as well as bioinformatics analysis, consistently demonstrated that the expression of ITGA10 was significantly upregulated while the other five DEGs (COL3A1, COL4A1, LOX, PDGFA, TGFBI) were downregulated in FGF2-treated fibroblasts. Meanwhile, 213 differentially expressed lncRNAs were identified and three key lncRNAs (HOXA-AS2, H19, and SNHG8) were highlighted in FGF2-treated fibroblasts. Conclusion The current study comprehensively analyzed the FGF2-responsive transcriptional profile and provided candidate mechanisms that may account for FGF2-mediated wound healing.

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Long non-coding RNA H19 promotes corneal neovascularization by targeting microRNA-29c

Cited by 20 publications

References 42 publications

Role of Endogenous Regulators of Hem- And Lymphangiogenesis in Corneal Transplantation

Role of Endogenous Regulators of Hem- And Lymphangiogenesis in Corneal Transplantation

Peer Review #1 of "RNA sequencing analysis of FGF2-responsive transcriptome in skin fibroblasts (v0.1)"

RNA sequencing analysis of FGF2-responsive transcriptome in skin fibroblasts

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