Long non-coding RNA H19, a negative regulator of microRNA-148b-3p, participates in hypoxia stress in human hepatic sinusoidal endothelial cells via NOX4 and eNOS/NO signaling

Zhu, Yiping; Tien, Ni; Lin, Jiayun; Zhang, Chihao; Zheng, Lei; Luo, Meng

doi:10.1016/j.biochi.2019.04.006

Cited by 29 publications

(11 citation statements)

References 32 publications

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“…The nuclear adapter LDB1 is the core component of a multiprotein transcription complex that regulates differentiation of multiple cell types, and plays an important role in regulating the transcription process responsible for maintaining hematopoietic stem cells [ 44 ]. Furthermore, NOX4 ( di value = 12.84), which contributes to hypoxia-induced angiogenesis by producing H 2 O 2 [ 45 ], was strongly selected. In addition, DPP4 (dipeptidyl peptidase 4) ( di value = 7.5), an important enzyme involved in glycolysis and the maintenance of glucose stability [ 46 ], has also been detected in the Tibetan sheep [ 23 ].…”

Section: Resultsmentioning

confidence: 99%

Selection Signatures Analysis Reveals Genes Associated with High-Altitude Adaptation in Tibetan Goats from Nagqu, Tibet

Jin

Lü

Fei

et al. 2020

Animals

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Tibetan goat is an ancient breed, which inhabits the adverse conditions of the plateaus in China. To investigate the role of selection in shaping its genomes, we genotyped Tibetan goats (Nagqu Prefecture, above 4500 m) and three lowland populations (Xinjiang goats, Taihang goats and Huanghuai goats). The result of PCA, neighbor-joining (N-J) tree and model-based clustering showed that the genetic structure between the Tibetan goat and the three lowland populations has significant difference. As demonstrated by the di statistic, we found that some genes were related to the high-altitude adaptation of Tibetan goats. Functional analysis revealed that these genes were enriched in the VEGF (vascular endothelial growth factor) signaling pathway and melanoma, suggesting that nine genes (FGF2, EGFR, AKT1, PTEN, MITF, ENPEP, SIRT6, KDR, and CDC42) might have important roles in the high-altitude adaptation of Nagqu Tibetan goats. We also found that the LEPR gene was under the strongest selection (di value = 16.70), and it could induce upregulation of the hypoxic ventilatory response. In addition, five genes (LEPR, LDB1, EGFR, NOX4 and FGF2) with high di values were analyzed using q-PCR. Among them, we found that LEPR, LDB1 and FGF2 exhibited higher expression in the lungs of the Tibetan goats; LEPR, EGFR and LDB1 exhibited higher expression in the hearts of the Huanghuai goat. Our results suggest that LEPR, LDB1, EGFR and FGF2 genes may be related to the high-altitude adaptation of the goats. These findings improve our understanding of the selection of the high-altitude adaptability of the Nagqu Tibetan goats and provide new theoretical knowledge for the conservation and utilization of germplasm resources.

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Section: Resultsmentioning

confidence: 99%

Selection Signatures Analysis Reveals Genes Associated with High-Altitude Adaptation in Tibetan Goats from Nagqu, Tibet

Jin

Lü

Fei

et al. 2020

Animals

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“…miR‐148b‐3p inhibition attenuates oxidized low‐density‐lipoprotein‐induced apoptosis in vascular smooth muscle cells . In hepatic sinusoidal endothelial cells, miR‐148b‐3p expression is dysregulated in response to hypoxia treatment and contributes to the modulation of hypoxic injury . Notably, miR‐148b‐3p inhibition protects cardiomyocytes from hypoxia/reoxygenation‐induced apoptosis in vitro .…”

Section: Discussionmentioning

confidence: 99%

“…35 In hepatic sinusoidal endothelial cells, miR-148b-3p expression is dysregulated in response to hypoxia treatment and contributes to the modulation of hypoxic injury. 36 Notably, miR-148b-3p inhibition protects cardiomyocytes from hypoxia/reoxygenation-induced apoptosis in vitro. 29 Moreover, in an in vivo model of myocardial ischaemia/reperfusion injury, miR-148b-3p inhibition decreases myocardial infarction area and improves myocardial function, benefits associated with suppression of apoptosis and oxidative stress in cardiomyocytes.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of microRNA‐148b‐3p alleviates oxygen‐glucose deprivation/reoxygenation‐induced apoptosis and oxidative stress in HT22 hippocampal neuron via reinforcing Sestrin2/Nrf2 signalling

et al. 2020

Clin Exp Pharma Physio

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MicroRNAs (miRNAs) have emerged as crucial regulators of neuronal injury during cerebral ischaemia/reperfusion injury. Various miRNAs are dysregulated during this pathological process; however, the precise role of these miRNAs in regulating neuronal injury remains largely unknown. In the current study, we explored the potential function of microRNA‐148b‐3p (miR‐148b‐3p) in regulating neuronal injury induced by oxygen‐glucose deprivation/reoxygenation (OGD/R) in vitro, a cellular model for mimicking cerebral ischaemia/reperfusion injury. We found that miR‐148b‐3p expression was significantly decreased in neurons in response to OGD/R exposure. Importantly, miR‐148b‐3p overexpression decreased cell viability and exacerbated apoptosis and reactive oxygen species (ROS) production in OGD/R‐exposed neurons. By contrast, miR‐148b‐3p inhibition improved cell viability and decreased apoptosis and ROS production in OGD/R‐exposed neurons. Notably, Sestrin2, a cytoprotective gene, was identified as a miR‐148b‐3p target gene. miR‐148b‐3p inhibition markedly increased Sestrin2 expression as well as the activation of nuclear factor erythroid‐2‐related factor 2 (Nrf2) antioxidant signalling. Moreover, silencing of Sestrin2 or Nrf2 significantly reversed the miR‐148‐3p‐inhibition‐mediated protective effect in OGD/R‐injured neurons. Overall, these results demonstrate that miR‐148b‐3p inhibition protects neurons from OGD/R‐induced apoptosis and ROS production through reinforcing Nrf2 antioxidant signalling via upregulation of Sestrin2. Our study indicates that the miR‐148b‐3p/Sestrin2/Nrf2 axis plays an important role in regulating neuronal injury and may serve as a potential therapeutic target for providing neuroprotection during cerebral ischaemia/reperfusion injury.

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“…Circulating long noncoding RNAs H19 and GAS5 are associated with type 2 diabetes but not with diabetic retinopathy: A preliminary study ABSTRACT www.bjbms.org H19 is also suggested to participate in hypoxia-induced stress response via regulating NADPH oxidase 4 (NOX4) and endothelial nitric oxide synthase/nitric oxide (eNOS/NO) signaling [14]. The serum level of another lncRNA, growth arrest-specific transcript 5 (GAS5), has been correlated with T2DM prevalence [15] and its downregulation has been associated with insulin resistance [16].…”

Section: Introductionmentioning

confidence: 99%

“…For example, Thomas et al [ 12 ] indicated H19 to participate in the regulation of endothelial-mesenchymal transition (EMT) in the diabetic retina through several mechanisms, including the positive regulation of microRNA-200b, which is one of the highly characterized angiomiRs involved in angiogenesis [ 13 ]. H19 is also suggested to participate in hypoxia-induced stress response via regulating NADPH oxidase 4 (NOX4) and endothelial nitric oxide synthase/nitric oxide (eNOS/NO) signaling [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Circulating long noncoding RNAs H19 and GAS5 are associated with type 2 diabetes but not with diabetic retinopathy: A preliminary study

Fawzy

Abdelghany

Toraih

et al. 2020

Bosn J of Basic Med Sci

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Recently, a wide range of biological and pathological roles of long noncoding RNAs (lncRNAs) have been discovered. However, the potential role of circulating lncRNAs H19 and GAS5 in type 2 diabetes mellitus (T2DM) and diabetic retinopathy (DR) is not clear. Here, we assessed the plasma levels of H19 and GAS5 lncRNAs in T2DM patients with/without DR and evaluated if H19 and GAS5 pre-treatment plasma levels are a predictor of early response to a single aflibercept dose in DR subgroup. Plasma lncRNA expression profiles of 119 T2DM patients (66 with DR and 53 without DR) and 110 healthy controls were determined by quantitative reverse transcription PCR. The association of lncRNA expression profiles with clinical features and aflibercept early response in DR patients was investigated. Relative H19 expression levels were significantly increased in T2DM group (including DR and non-DR subgroups) vs. controls, while GAS5 levels were decreased in T2DM group (p < 0.001).

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Long non-coding RNA H19, a negative regulator of microRNA-148b-3p, participates in hypoxia stress in human hepatic sinusoidal endothelial cells via NOX4 and eNOS/NO signaling

Cited by 29 publications

References 32 publications

Selection Signatures Analysis Reveals Genes Associated with High-Altitude Adaptation in Tibetan Goats from Nagqu, Tibet

Selection Signatures Analysis Reveals Genes Associated with High-Altitude Adaptation in Tibetan Goats from Nagqu, Tibet

Inhibition of microRNA‐148b‐3p alleviates oxygen‐glucose deprivation/reoxygenation‐induced apoptosis and oxidative stress in HT22 hippocampal neuron via reinforcing Sestrin2/Nrf2 signalling

Circulating long noncoding RNAs H19 and GAS5 are associated with type 2 diabetes but not with diabetic retinopathy: A preliminary study

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