Long Non-Coding RNA FLVCR1-AS1 Acts as miR-493-3p Sponge to Modulate Cancer Cell Proliferation, Invasion and Migration in Colorectal Cancer

Abstract

This study aimed to investigate the effect and mechanism of long non-coding RNA FLVCR1-AS1 on proliferation, invasion and migration of colorectal cancer. QRT-PCR was used to detect the expression of FLVCR1-AS1 in colorectal cancer. The target genes of FLVCR1-AS1 were predicted using the StarBase and Luciferase assay. The biological function of miR-493-3p through a series of in vitro experiments. We examined the expression of FLVCR1-AS1 in colorectal cancer cell lines was remarkably increased. Interference with FLVCR1-AS1 inhibits proliferation, invasion and migration of colorectal cancer cell lines. MiR-493-3p is a targeted miRNA of lncRNA FLVCR1-AS1, and overexpression of miR-493-3p inhibits proliferation of colorectal cancer cells, invasion and migration of colorectal cancer cells, and it can be reversed by lncRNA FLVCR1-AS1. LncRNA FLVCR1-AS1 acts as miR-493-3p sponge to modulate cancer cell proliferation, invasion and migration in colorectal cancer.

FLVCR1-AS1 and FBXL19-AS1: Two Putative lncRNA Candidates in Multiple Human Cancers

“…The authors suggested targeting FLVCR1-AS1 as a novel approach for treating CRC [ 32 ]. In another study, LncFLVCR1-AS1 acts as a miR-493-3p sponge to modulate cancer cell proliferation, invasion, and migration in CRC [ 33 ].…”

“…The key roles in gene regulation of lncRNAs affecting cellular homeostasis leading to tumorigenesis, cancer progression, and distant metastasis have been well established in many studies. Increasing evidence has demonstrated that lncRNAs participate in various cancer development and progression, very often acting as sponges of miRNAs to regulate their target gene expression ( Figure 5 ) [ 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 ].…”

“…In addition, According to the lncLocator database ( , accessed on 26 January 2021), FLVCR1-AS1 is located in the nucleus and cytoplasm ( Table 1 ). FLVCR1-AS1 has been described as a new tumor suppressor as its impaired expression can lead to the development and spread of various cancers such as cholangiocarcinoma (CCA) [ 28 ], hepatocellular carcinoma (HCC) [ 29 , 30 ], gastric cancer (GC) [ 31 ], colorectal cancer (CRC) [ 32 , 33 ], glioma and glioblastoma (GBM) [ 34 , 35 ], non-small cell lung cancer (NSCLC) [ 36 , 37 ], ovarian cancer (OC) [ 38 ], breast cancer (BC) [ 39 ], and osteosarcoma (OS) [ 40 ], and in October 2021, was reported in pancreatic cancer (PC) [ 41 ].…”