Long non-coding RNA DSCAM-AS1 contributes to the tumorigenesis of cervical cancer by targeting miR-877-5p/ATXN7L3 axis

Liang, Jie; Zhang, Shujuan; Wang, Wei; Xu, Yan; Kawuli, Atikan; Lü, Jiaqi; Xiu, Xuemei

doi:10.1042/bsr20192061

Cited by 41 publications

(39 citation statements)

References 26 publications

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“…Previous studies revealed a reduction of miR‐877‐5p expression levels in malignant tumors 27,28 . The present study showed miR‐877‐5p was suppressed in IL‐1β‐stimulated chondrocytes and the proximal tibia of IL‐1β‐treated mice.…”

Section: Discussionsupporting

confidence: 64%

“…* p < 0.05 bioinformatics algorithms deduced a highly conserved sequence in the Previous studies revealed a reduction of miR-877-5p expression levels in malignant tumors. 27,28 The present study showed miR- IL-1β as an inflammatory cytokine is widely reported to establish both in vivo and in vitro models of OA by accelerating articular cartilage degeneration and chondrocyte apoptosis. 24 Usually, overactivation of the inflammatory response is accompanied by IL-1βinduced OA-like lesions.…”

Section: Discussionmentioning

confidence: 55%

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miR‐877‐5p alleviates chondrocyte dysfunction in osteoarthritis models via repressing FOXM1

Zhu

Deng

Gao

et al. 2020

The Journal of Gene Medicine

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Background: The present study aimed to investigate whether forkhead box M1 (FOXM1), as a putative target of miR-877-5p, participated in interleukin (IL)-1βinduced cartilage degeneration in experimental osteoarthritis (OA) models in vitro and in vivo. Methods: In vitro and in vivo models of OA were established using IL-1β treated primary mouse chondrocytes and anterior cruciate ligament transection (ACLT) operation in mice. miR-877-5p mimics or agomir-miR-877-5p were used as therapeutic agents in both in vitro and in vivo models of OA. Cell viability and apoptosis were evaluated using cell counting kit-8 and terminal deoxynucleotidyl transferase dUTP nick end labeling staining, respectively. A quantitative reverse transcriptasepolymerase chain reaction and western blotting were used to measure gene and protein expression, respectively. Results: FOXM1 was up-regulated in IL-1β-stimulated chondrocytes and the proximal tibia of ACLT-operated mice. Bioinformatics algorithms deduced a highly conserved sequence in the 3'-UTR of FOXM1 that could be bound with miR-877-5p. A luciferase assay indicated that miR-877-5p directly targeted the 3'-UTR of FOXM1. Overexpression of miR-877-5p could reduce protein expression of FOXM1 in chondrocytes. Concurrently, IL-1β-evoked up-regulation of FOXM1 protein expression was neutralized in chondrocytes following transfection with miR-877-5p mimics. miR-877-5p mimics or agomir-miR-887-5p could inhibit IL-1β-induced inflammation in both in vitro and in vivo models of OA. miR-877-5p might have beneficial effects on the synthesis of cartilage matrix via the promotion of SRY-box transcription factor 9 and type II collagen expression and inhibition of matrix metalloproteinase 9 expression. Conclusions: miR-877-5p can improve chondrocyte function in both in vivo and in vitro models of OA, based on post-transcriptional repression of FOXM1 as a postulated molecular mechanism.

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Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 55%

miR‐877‐5p alleviates chondrocyte dysfunction in osteoarthritis models via repressing FOXM1

Zhu

Deng

Gao

et al. 2020

The Journal of Gene Medicine

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“…A previous study showed that circ-BIRC6 was highly expressed in HCC tissues and cells, and circ-BIRC6 knockdown inhibited the progression of HCC via regulating the miR-3918/ Bcl2 axis. 13 Moreover, miR-877-5p was reported to be downregulated and play an anti-cancer role in many cancers, such as cervical cancer, 31 glioblastoma, 32 renal cell carcinoma, 33 and colorectal cancer, 34 as well as HCC. 17 In agreement with these findings, we also observed that circ-BIRC6 expression was enhanced, and the miR-877-5p level was reduced in HCC tissue samples and cells.…”

Section: Discussionmentioning

confidence: 99%

<p>Paclitaxel Suppresses Hepatocellular Carcinoma Tumorigenesis Through Regulating Circ-BIRC6/miR-877-5p/<em>YWHAZ</em> Axis</p>

Liu

Guo

Shen

et al. 2020

OTT

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Background: Paclitaxel is an effective chemotherapeutic agent for the treatment of cancer patients. Accumulating evidence suggests that circular RNAs (circRNAs) play critical roles in the occurrence and development of human cancers. However, there are few studies on interactions between paclitaxel and circRNAs in hepatocellular carcinoma (HCC). Materials and Methods: Cell counting kit-8 (CCK-8) assay and colony formation assay were conducted to determine cell proliferation. Cell apoptosis was assessed by flow cytometry. The expression levels of circRNA baculoviral IAP repeat-containing 6 (circ-BIRC6), microRNA-877-5p (miR-877-5p), and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta (YWHAZ) were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The mice xenograft model was established to investigate the roles of circ-BIRC6 and paclitaxel in vivo. The interaction between miR-877-5p and circ-BIRC6 or YWHAZ was predicted by bioinformatics analysis and verified by dual-luciferase reporter assay. Western blot assay was applied for measuring the protein expression of YWHAZ. Results: Paclitaxel suppressed HCC tumorigenesis through decreasing cell proliferation and accelerating apoptosis. Circ-BIRC6 and YWHAZ were upregulated, and miR-877-5p was downregulated in HCC tissues and cells. Paclitaxel treatment inhibited the expression of circ-BIRC6 and YWHAZ while promoted the expression of miR-877-5p. Circ-BIRC6 overexpression or miR-877-5p interference reversed the inhibitory effect of paclitaxel on HCC tumorigenesis. Moreover, miR-877-5p could specially bind to YWHAZ, and its knockdown abated the suppressive effect of circ-BIRC6 depletion on HCC tumorigenesis. Additionally, YWHAZ was identified as a direct target of miR-877-5p. Besides, circ-BIRC6 functioned as a molecular sponge of miR-877-5p to regulate YWHAZ expression. Conclusion: Paclitaxel limited HCC tumorigenesis via modulating circ-BIRC6/miR-877-5p/YWHAZ axis, providing a novel therapeutic approach for the treatment of HCC.

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“…MiR-877-5p had been proved to exert a repressive role in several cancers. Liang et al uncovered that lncRNA DSCAM-AS1 inhibition elevated the expression of miR-877-5p, thereby repressing invasion, migration, and proliferation of cervical cancer cells [ 13 ]. Report of Xie et al revealed that lncRNA TRG-AS1 accelerated proliferation of glioblastoma through elevating SUZ12 expression by adsorbing miR-877-5p [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

“…Studies have demonstrated that miRs exert vital regulatory roles in cellular processes, such as cell differentiation, invasion, migration, autophagy, and apoptosis [ 12 ]. MiR-877-5p had been proved to play an anti-tumor impact on cervical cancer [ 13 ] and glioblastoma [ 14 ]. Also, miR-877-5p constrained malignant behaviors of HCC cells [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Circular RNA hsa_circ_0061395 accelerates hepatocellular carcinoma progression via regulation of the miR-877-5p/PIK3R3 axis

Bian

Liu³

et al. 2021

Cancer Cell Int

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Background Circular RNA hsa_circ_0061395 (circ_0061395) has been reported to accelerate the advancement of hepatocellular carcinoma (HCC). However, the regulatory mechanism by which circ_0061395 modulates the progression of HCC is unclear. Methods The morphology and size of exosomes were analyzed by transmission electron microscope (TEM) and nanoparticle-tracking analysis (NTA). Protein levels were detected by western blotting. Expression levels of circ_0061395, microRNA (miR)-877-5p, and phosphoinositide-3-kinase regulatory subunit 3 (PIK3R3) mRNA were assessed by quantitative real time polymerase chain reaction (qRT-PCR). The proliferation, invasion, migration, cell cycle progression, and apoptosis were analyzed by cell counting kit-8 (CCK-8), plate clone, transwell, or flow cytometry assays. The targeting relationship between circ_0061395 or PIK3R3 and miR-877-5p was verified using the dual-luciferase reporter and/or RNA immunoprecipitation (RIP) assays. Xenograft assay was performed to confirm the biological function of circ_0061395 in HCC. Results Circ_0061395 was upregulated in HCC tissues, serum, cells, and serum-derived exosomes. Circ_0061395 silencing decreased tumor growth in vivo, and induced cell cycle arrest, apoptosis, repressed proliferation, invasion, and migration of HCC cells in vitro. MiR-877-5p was downregulated while PIK3R3 was upregulated in HCC. Circ_0061395 regulated PIK3R3 expression via competitively binding to miR-877-5p. MiR-877-5p inhibitor overturned circ_0061395 knockdown-mediated influence on malignant behaviors of HCC cells. PIK3R3 overexpression reversed the suppressive influence of miR-877-5p mimic on malignant behaviors of HCC cells. Conclusion Circ_0061395 facilitated HCC progression via regulating the miR-877-5p/PIK3R3 axis, providing a new perspective on the advancement of HCC.

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Long non-coding RNA DSCAM-AS1 contributes to the tumorigenesis of cervical cancer by targeting miR-877-5p/ATXN7L3 axis

Cited by 41 publications

References 26 publications

miR‐877‐5p alleviates chondrocyte dysfunction in osteoarthritis models via repressing FOXM1

miR‐877‐5p alleviates chondrocyte dysfunction in osteoarthritis models via repressing FOXM1

<p>Paclitaxel Suppresses Hepatocellular Carcinoma Tumorigenesis Through Regulating Circ-BIRC6/miR-877-5p/<em>YWHAZ</em> Axis</p>

Circular RNA hsa_circ_0061395 accelerates hepatocellular carcinoma progression via regulation of the miR-877-5p/PIK3R3 axis

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