Long non-coding RNA DNM3OS promotes tumor progression and EMT in gastric cancer by associating with Snail

Wang, Shuchang; Ni, Bo; Zhang, Zizhen; Wang, Chaojie; Wo, Lulu; Zhou, Chenyu; Zhao, Qian; Zhao, Enyue

doi:10.1016/j.bbrc.2019.02.030

Cited by 21 publications

(19 citation statements)

References 27 publications

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“…Our results demonstrated that silencing of DNM3OS in GIST cells reduced cell viability and the mitotic index. These results con rmed the role of DNM3OS as an oncogenic lncRNA in the progression of various cancers, consistent with the results of previous studies [39,43].…”

Section: Discussionsupporting

confidence: 92%

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Integrated Analysis of Long Non-Coding RNAs and mRNAs Associated With Malignant Transformation of Gastrointestinal Stromal Tumors

Yin

Dai

et al. 2020

Preprint

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Abstract Background: Malignant transformation of gastrointestinal stromal tumors (GISTs) is correlated with poor prognosis; however, the underlying biological mechanism is not well understood. Methods: In the present study, low-risk (LR) GISTs, GISTs categorized as high-risk based on tumor size (HBS), and on mitotic rate (HBM) were collected for RNA sequencing. Candidate hub lncRNAs were selected by Oncomine analysis. Expression of a selected hub lncRNA, DNM3OS, and its correlation with patients’ prognosis were analyzed using FISH staining, followed with the determination of function and underlying mechanism. Results: Our results revealed a series of key pathways and hub lncRNAs involved in the malignant transformation of GISTs. Oncomine analysis revealed a tight association between clinical signatures and DNM3OS and suggested that DNM3OS is a hub lncRNA that is involved in the Hippo signaling pathway. In addition, DNM3OS was upregulated in HBS, HBM, and HBS/M GIST and correlated with worse prognosis in patients with GISTs. In addition, DNM3OS promoted GIST cell proliferation and mitosis by regulating the expression of GLUT4 and CD36. Conclusions: These results improve our understanding of the malignant transformation of GISTs and unveil a series of hub lncRNAs in GISTs.

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Section: Discussionsupporting

confidence: 92%

“…DNM3OS regulates gene expression post-transcriptionally, and overexpression of DNM3OS has been shown to be associated with tumor progression and radio resistance in ovarian cancer, gastric cancer, and esophageal squamous cell carcinoma [39,41,42]. Our results indicated that DNM3OS was higher in GISTs tissues than in adjacent normal tissues.…”

Section: Discussionmentioning

confidence: 54%

Integrated Analysis of Long Non-Coding RNAs and mRNAs Associated With Malignant Transformation of Gastrointestinal Stromal Tumors

Yin

Dai

et al. 2020

Preprint

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“…HAGLROS is pointed out as a highly expressed factor in several cancers, such as osteosarcoma [17] and gastric cancer [20]. However, whether HAGLROS is also dysregulated in NPC remains unknown.…”

Section: Correlation Between Haglros Level and Clinicopathological Fementioning

confidence: 99%

RETRACTED ARTICLE: Upregulation of lncRNA HAGLROS enhances the development of nasopharyngeal carcinoma via modulating miR-100/ATG14 axis-mediated PI3K/AKT/mTOR signals

Zhang

2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…21 The depletion of lncRNA DNM3OS inhibits the migration and invasion of gastric cancer cells by suppressing snail-mediated EMT. 22 In human U251 glioma cells, β-Asarone suppressed EMT and invasion through the inhibition of the splicing factor HnRNP A2/B1. 23 Furthermore, it is noteworthy that there is growing evidence that autophagy inhibitors could enhance the efficacy of treatment of different cancer types.…”

Section: Introductionmentioning

confidence: 99%

<p>Meg3 Induces EMT and Invasion of Glioma Cells via Autophagy</p>

Yang

Bian

et al. 2020

OTT

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Background: Glioma is one of the most common malignant tumors. Glioblastoma (grade IV) is considered the most malignant form of human brain tumors. Maternal expression gene 3 (Meg3) encodes a non-coding RNA (ncRNA) that plays an important role in the development and progression of cancer. However, the role of Meg3 in glioma cells remains largely unclear. Methods: Reverse transcription-quantitative (RT-q) PCR was conducted to evaluate the mRNA expression related to cell autophagy and EMT while protein expression was detected by Western blotting. Staining of acidic vacuoles and immunofluorescence staining were used to detect autophagy. The ability of cells to migrate and invade was detected by Transwell migration and invasion assays. Results: In the present study, it was found that the overexpression of Meg3 induced EMT, migration and invasion of glioma cells, whereas Meg3 overexpression induced autophagy of glioma cells. More importantly, the inhibition of autophagy impaired the EMT of glioma cells. In addition, Meg3-induced EMT, migration and invasion could be partially reversed by autophagy inhibitors, chloroquine (CQ) and Lys05, in glioma cells. Conclusion: All data suggest that Meg3 induces EMT and invasion of glioma cells via autophagy. Overall, the findings of the present study demonstrate the importance of Meg3 in the molecular etiology of glioma, which also indicate its potential applications in the treatment of glioma.

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Long non-coding RNA DNM3OS promotes tumor progression and EMT in gastric cancer by associating with Snail

Cited by 21 publications

References 27 publications

Integrated Analysis of Long Non-Coding RNAs and mRNAs Associated With Malignant Transformation of Gastrointestinal Stromal Tumors

Integrated Analysis of Long Non-Coding RNAs and mRNAs Associated With Malignant Transformation of Gastrointestinal Stromal Tumors

RETRACTED ARTICLE: Upregulation of lncRNA HAGLROS enhances the development of nasopharyngeal carcinoma via modulating miR-100/ATG14 axis-mediated PI3K/AKT/mTOR signals

<p>Meg3 Induces EMT and Invasion of Glioma Cells via Autophagy</p>

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