Long non‑coding RNA DLEU1 promotes cell proliferation of glioblastoma multiforme

Abstract

Glioblastoma multiforme (GBM) is the most common malignant tumor with high morbidity and mortality. This study investigated the role of long non-coding rnas (lncrnas) in glioblastomagenesis progression. using the GSe2223 and GSe59612 datasets, and rna sequencing data of GBM from The cancer Genome atlas, differentially expressed (de) genes including de messenger rnas (demrnas) and delncrnas between GBM and normal controls were identified. Based on the competing endogenous RNA hypothesis, delncrna-micro rna (mirna)-demrna interactions were obtained by target gene prediction. Gene ontology (Go) and Kyoto encyclopedia of Gene and Genomes pathway analysis of demrnas in the delncrna-mirna-demrna network was performed. expression and function analyses of delncrnas were performed by reverse transcription-polymerase chain reaction (rT-Pcr) and an established viability assay, respectively. In total, 712 DE genes were identified. Significant upregulation of lncRNA deleted in lymphocytic leukemia 1 (DLEU1) was revealed in GBM and a number of other types of cancer. dleu1 interacted with 315 mirnas and 105 demrnas. The demrnas were mainly enriched in tumorigenesis-associated Go terms (angiogenesis, positive regulation of cell proliferation, positive regulation of fibroblast apoptotic processes and regulation of neutrophil migration) and pathways (Hippo signaling pathway, cancer pathways, and Wnt signaling pathway). correlation analysis revealed that mrna TnF receptor associated factor 4 (TraF4) was associated with dleu1 expression. rT-Pcr demonstrated that the expression levels of dleu1 and TraF4 were increased in GBM tissues. Small interfering rna demonstrated that silencing dleu1 downregulated TraF4. The viability of GBM cells was significantly decreased following RNA interference with dleu1 and TraF4 production. The results demonstrate that dleu1 and TraF4 is highly expressed in GBM tissues and promotes proliferation of GBM cells. it may act as a competing endogenous RNA and influence tumorigenesis of GBM.

Exploring the potential biomarkers for prognosis of glioblastoma via weighted gene co-expression network analysis

“…Further determination of the key gene requires a combination of its expression, typicality in previous studies, and previous research experience in our lab. Among the 14 candidate genes we selected, most genes have been confirmed to be related to the prognosis of GBM, such as: SPAG4, FKBP1B, DLEU1, PRKAR2B, NRL, CD24, GAS6; some genes have not been clearly studied to be related to the occurrence of any known tumors, such as CAMSAP2; the remaining genes have not been significantly expressed in GBM, such as CORO6 ( Lo et al, 2009 ; Sun et al, 2021 ; Wang et al, 2019 ; Zhao et al, 2019 ). Finally, we found that four genes (DARS/GDI2/P4HA2/TRUB1) had an impact on the survival rate of patients with GBM, and were also confirmed to be related to tumorigenesis.…”

The Research Progress in Physiological and Pathological Functions of TRAF4

“…High expression of TRAF4 significantly increased cyclinD1 and c-myc protein expression levels in cancer cells and activated the Wnt/β-catenin signaling pathway ( 75 ). DLEU1 can act as a competitive endogenous RNA that promotes elevated TRAF4 expression levels, promotes cancer cell proliferation through the Hippo signaling pathway and Wnt/β-catenin signaling pathway and regulate cell migration ( 79 ).…”

“…Increased expression levels of DLEU1 and TRAF4 in glioblastoma multiforme (GBM) tissues promote GBM cell proliferation and regulate cell migration through the Hippo signaling pathway and Wnt signaling pathway. DLEU1 in GBM can act as a competitive endogenous RNA, thereby promoting GBM tumorigenesis ( 79 ).…”

Advances in Understanding the LncRNA-Mediated Regulation of the Hippo Pathway in Cancer

“…Glioma is the most common type of primary brain tumor, accounting for 51.4% of all primary brain tumors, and glioblastoma is the most aggressive glioma, with high mortality in adults. 111,112 In the GSE2223 and GSE59612 datasets Wang et al 113 screened 712 lncRNAs dysregulated in glioblastoma multiforme compared with normal control tissues. Among these lncRNAs, DLEU1 was found to be significantly upregulated and to interact with 315 miRNAs and 105 mRNAs associated mainly with tumorigenesisrelated terms and pathways, including the Hippo signaling pathway.…”