Long Non-coding RNA DLEU1 Promotes Cell Proliferation, Invasion, and Confers Cisplatin Resistance in Bladder Cancer by Regulating the miR-99b/HS3ST3B1 Axis

Li, Yongzhi; Shi, Benkang; Dong, Feihong; Zhu, Xiao‐Feng; Liu, Bing; Liu, Yili

doi:10.3389/fgene.2019.00280

Cited by 31 publications

(29 citation statements)

References 30 publications

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“…It has been documented that DLEU1 functions as an oncogene by regulating target miRNA. For instance, DLEU1 was found to be upregulated in bladder cancer tissues [6]. Patients with high DLEU1 expression exhibit a shorter survival time.…”

Section: Discussionmentioning

confidence: 98%

“…Increasingly, many lncRNAs have been reported to play important roles in multiply biological processes of osteosarcoma including tumour cell proliferation, invasion, migration, and chemoresistance [3,4]. LncRNA DLEU1 is overexpressed and exerts an oncogene function by regulating cell proliferation, migration and invasion in several types of cancers including bladder cancer [6], cervical cancer [7], oral squamous cell carcinoma [8], non-small cell lung cancer [9], and colorectal cancer [10]. However, the role of DLEU1 in osteosarcoma remains unclear.…”

Section: Introductionmentioning

confidence: 99%

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Long noncoding RNA DLEU1 aggravates osteosarcoma carcinogenesis via regulating the miR-671-5p/DDX5 axis

Chen

Zhang

Wang

2019

Artificial Cells, Nanomedicine, and Biotechnology

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Long non-coding RNAs (LncRNAs) have been proven to be involved in the progression of osteosarcoma. LncRNA DLEU1 was found to act as an oncogene in various types of cancer. In this study, we aimed to explore the biological functions of DLEU1 in osteosarcoma and the specific mechanism. Our results showed that DLEU1 was highly expressed in osteosarcoma tissue specimens and cells. Downregulation of DLEU1 in osteosarcoma cells inhibited the cell proliferation, migration and invasion. Further mechanistic analysis and functional assays demonstrated that DLEU1 exerted its role by sponging microRNA (miR)-671-5p in osteosarcoma cells. Moreover, DEAD-box helicase 5 (DDX5) was confirmed as the target of miR-671-5p. Furthermore, rescue assays indicated that miR-671-5p inhibitor significantly reversed the effects of DLEU1 knockdown on osteosarcoma cell proliferation and invasion while restoration of DDX5 rescued the proliferation and invasion of osteosarcoma cells transfected with miR-671-5p mimics. In conclusion, DLEU1 acted as an oncogene in osteosarcoma by directly sponging miR-671-5p and regulating the expression of DDX5. These findings suggested that DLEU1 might be a novel therapeutic target in the treatment of osteosarcoma. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA DLEU1 aggravates osteosarcoma carcinogenesis via regulating the miR-671-5p/DDX5 axis

Chen

Zhang

Wang

2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…The same function is suggested in BC where miR-99b shows significant downregulation [26] and targets, among other genes, FGFR3 [115] which is constitutively activated in NMIBC [116]. Sponging miR-99b by DLEU1 results in increased expression of oncogenic membrane protein HS3ST3B1 [26]. Although limited information about the exact function in BC is known, in other tumors HS3ST3B1 is associated mainly with EMT [117].…”

Section: Dleu1mentioning

confidence: 79%

“…In BC, DLEU1 is significantly increased and induces cell proliferation, invasion, and cisplatin resistance. The mechanism involves sponging miR-99b [26], which was described as a tumor suppressive factor in multiple cancers [114,115]. The same function is suggested in BC where miR-99b shows significant downregulation [26] and targets, among other genes, FGFR3 [115] which is constitutively activated in NMIBC [116].…”

Section: Dleu1mentioning

confidence: 99%

lncRNA and Mechanisms of Drug Resistance in Cancers of the Genitourinary System

Barth

Juráček

Slabý

et al. 2020

Cancers

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Available systemic treatment options for cancers of the genitourinary system have experienced great progress in the last decade. However, a large proportion of patients eventually develop resistance to treatment, resulting in disease progression and shorter overall survival. Biomarkers indicating the increasing resistance to cancer therapies are yet to enter clinical routine. Long non-coding RNAs (lncRNA) are non-protein coding RNA transcripts longer than 200 nucleotides that exert multiple types of regulatory functions of all known cellular processes. Increasing evidence supports the role of lncRNAs in cancer development and progression. Additionally, their involvement in the development of drug resistance across various cancer entities, including genitourinary malignancies, are starting to be discovered. Consequently, lncRNAs have been suggested as factors in novel therapeutic strategies to overcome drug resistance in cancer. In this review, the existing evidences on lncRNAs and their involvement in mechanisms of drug resistance in cancers of the genitourinary system, including renal cell carcinoma, bladder cancer, prostate cancer, and testicular cancer, will be highlighted and discussed to facilitate and encourage further research in this field. We summarize a significant number of lncRNAs with proposed pathways in drug resistance and available reported studies.

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“…ncRNA including miRNA, long ncRNA (lncRNA) and circular RNA (circRNA) [ 4–6 ]. miRNA and lncRNA have been extensively studied in various tumors, such as glioblastoma [ 7 ], gastric cancer [ 8 ], liver cancer [ 9 ], colorectal cancer [ 10 ] and bladder cancer [ 11 ]. However, their role in oncogenesis and development of circRNA is still largely unclear.…”

Section: Introductionmentioning

confidence: 99%

Hsa_circ_0026134 expression promoted TRIM25- and IGF2BP3-mediated hepatocellular carcinoma cell proliferation and invasion via sponging miR-127-5p

et al. 2020

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Increasing evidence shows that circular RNAs (circRNAs) play a regulatory role in cancer. In the present study, we aimed to investigate the characteristics and effects of hsa_circ_0026134 in hepatocellular carcinoma (HCC). We investigated hsa_circ_0026134 expression in 20 pairs of clinical tissues from HCC patients; expression of hsa_circ_0026134 in different cell lines; effect of hsa_circ_0026134 on proliferation and invasion of HCC cell lines; and the regulatory mechanisms and interactions among hsa_circ_0026134, miR-127-5p, tripartite motif-containing protein 25 (TRIM25) and insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3). hsa_circ_0026134 expression was increased in HCC samples and cell lines. Downregulation of hsa_circ_0026134 attenuated HCC cell proliferation and metastatic properties. Micro (mi)RNA (miR)-127-5p was sponged by hsa_circ_0026134. Rescue experiments indicated that inhibition of miR-127-5p expression promoted cell proliferation and invasion even after hsa_circ_0026134 silencing. TRIM25 and IGF2BP3 were targets of miR-127-5p. Overexpression of TRIM25 or IGF2BP3 promoted cell proliferation and invasion in cells overexpressing miR-127-5p. Downregulation of hsa_circ_0026134 suppressed TRIM25- and IGF2BP3-mediated HCC cell proliferation and invasion via promotion of miR-127-5p expression, which have been confirmed by luciferase reporter assay. This study provides a new treatment target for HCC.

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Long Non-coding RNA DLEU1 Promotes Cell Proliferation, Invasion, and Confers Cisplatin Resistance in Bladder Cancer by Regulating the miR-99b/HS3ST3B1 Axis

Cited by 31 publications

References 30 publications

Long noncoding RNA DLEU1 aggravates osteosarcoma carcinogenesis via regulating the miR-671-5p/DDX5 axis

Long noncoding RNA DLEU1 aggravates osteosarcoma carcinogenesis via regulating the miR-671-5p/DDX5 axis

lncRNA and Mechanisms of Drug Resistance in Cancers of the Genitourinary System

Hsa_circ_0026134 expression promoted TRIM25- and IGF2BP3-mediated hepatocellular carcinoma cell proliferation and invasion via sponging miR-127-5p

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