Long Non-coding RNA DLEU1 Promotes Proliferation and Invasion by Interacting With miR-381 and Enhancing HOXA13 Expression in Cervical Cancer

Abstract

Although growing evidence has demonstrated that the long non-coding RNA DLEU1 is involved in the progression of various cancers, its functional role and underlying mechanisms have not been explored in cervical cancer (CC). In this study, we found that DLEU1 was up-regulated in both CC tissues and CC cell lines, and overexpression of DLEU1 was significantly correlated with shorter patient survival. Knockdown of DLEU1 suppressed CC cell proliferation and invasion, whereas overexpression of DLEU1 promoted the proliferation and invasion of CC cells. Bioinformatics analysis was used to elucidate the potential correlation between DLEU1 and miR-381. Moreover, qRT-PCR analysis, luciferase reporter assay and RNA immunoprecipitation assay confirmed that DLEU1 inhibited the expression of miR-381, and revealed a direct interaction between DLEU1 and miR-381. In addition, we demonstrated that miR-381 directly targeted HOXA13 in CC cells. The restoration of HOXA13 expression reversed DLEU1 knockdown or miR-381 overexpression-mediated suppression of cell proliferation and invasion. These results suggested that DLEU1 can promote CC cell proliferation and invasion via the miR-381/HOXA13 axis.

The Communication between the PI3K/AKT/mTOR Pathway and Y-Box Binding Protein-1 in Gynecological Cancer

“…A systematic analysis of the literature showed that among currently identified lncRNAs, there are two overlapping lncRNAs in gynecological malignancies. These are MEG3 (maternally expressed gene 3) [81][82][83] and DLEU1 (deleted in lymphocytic leukemia 1) [84][85][86], which have been identified in all three gynecological cancers. MEG3 is a maternally imprinted gene functioning as a tumor suppressor.…”

Long noncoding RNA DLEU1 aggravates osteosarcoma carcinogenesis via regulating the miR-671-5p/DDX5 axis

“…Increasingly, many lncRNAs have been reported to play important roles in multiply biological processes of osteosarcoma including tumour cell proliferation, invasion, migration, and chemoresistance [3,4]. LncRNA DLEU1 is overexpressed and exerts an oncogene function by regulating cell proliferation, migration and invasion in several types of cancers including bladder cancer [6], cervical cancer [7], oral squamous cell carcinoma [8], non-small cell lung cancer [9], and colorectal cancer [10]. However, the role of DLEU1 in osteosarcoma remains unclear.…”

Retracted Article: Long noncoding RNA DLEU1 promotes cell proliferation and migration of Wilms tumor through the miR-300/HOXC8 axis

“…13 LncRNA deleted in lymphocytic leukemia 1 (DLEU1) is an identied oncogenic lncRNA in various cancers, such as lung cancer, colorectal cancer, cervical cancer, endometrial cancer, and ovarian carcinoma. [14][15][16][17][18] DLEU1 expression is upregulated in colorectal cancer tissues, the higher expression of DLEU1 indicates lower survival rate and poorer prognosis. 15 DLEU1 promotes tumorigenesis and progression of non-small cell lung cancer (NSCLC) and might be a promising therapeutic target for NSCLC.…”