Long non-coding RNA DARS-AS1 promotes tumorigenesis of non-small cell lung cancer via targeting miR-532-3p

Liu, Dongguo; Li, Haibo; Jiang, Zongpeng; Chen, Minglian; Gao, Shuncui

doi:10.23736/s0026-4806.19.06198-6

Cited by 19 publications

(17 citation statements)

References 4 publications

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“…LncRNAs regulate mRNAs by acting as a sponge of miRNAs, thereby regulating modulating progression [ 11 , 12 ]. Therefore, we speculate that the carcinogenic effect of DARS-AS1 in HCC may be achieved by regulating miRNAs.…”

Section: Resultsmentioning

confidence: 99%

“…Here, we investigated the role of DARS-AS1 in regulating HCC progression. According to reports, DARS-AS1 is abnormally expressed in tumor tissues and can be used as a prognostic indicator for various tumors [ 9 , 10 , 11 , 12 ]. Functionally, DARS-AS1 modulates diversified cellular biological processes, including tumor cell proliferation, migration, invasion and apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, lncRNA HANR facilitates HCC tumorigenesis and chemoresistance to adriamycin [ 8 ]. Similarly, as a lncRNA, DARS1 antisense RNA 1 (DARS-AS1)’s carcinogenesis has been testified in ovarian cancer, clear cell renal cell carcinoma, non-small cell lung cancer, and cervical cancer [ 9 , 10 , 11 , 12 ]. However, its carcinogenesis in HCC has rarely drawn public’s attention.…”

Section: Introductionmentioning

confidence: 99%

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LncRNA DARS-AS1 aggravates the growth and metastasis of hepatocellular carcinoma via regulating the miR-3200-5p-Cytoskeleton associated protein 2 (CKAP2) axis

et al. 2021

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

LncRNA DARS-AS1 aggravates the growth and metastasis of hepatocellular carcinoma via regulating the miR-3200-5p-Cytoskeleton associated protein 2 (CKAP2) axis

et al. 2021

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“…Increasing evidences have revealed that lncRNAs are dysregulated in various cancers such as lung carcinoma, bladder cancer, gastric carcinoma, hepatocellular carcinoma, and also osteosarcoma [ 19 – 24 ]. Studies revealed that DARS-AS1 exerted oncogenic roles in human tumors such as thyroid cancer, myeloma, lung cancer, and ovarian cancer [ 25 – 28 ]. For example, Zheng et al [ 27 ] revealed that DARS-AS1 level was increased in thyroid tumor specimens and was associated with poor prognosis, distant metastasis, and pathological stage, and DARS-AS1 facilitated thyroid tumor cell migration and proliferation via regulating miR-129.…”

Section: Introductionmentioning

confidence: 99%

“…For example, Zheng et al [ 27 ] revealed that DARS-AS1 level was increased in thyroid tumor specimens and was associated with poor prognosis, distant metastasis, and pathological stage, and DARS-AS1 facilitated thyroid tumor cell migration and proliferation via regulating miR-129. Liu et al [ 28 ] demonstrated that DARS-AS1 induced nonsmall cell lung tumor progression through modulating miR-532-3p. Yan [ 26 ] showed that DARS-AS1 was upregulated via HIF-1 in myeloma.…”

Section: Introductionmentioning

confidence: 99%

lncRNA DARS-AS1 Promoted Osteosarcoma Progression through Regulating miR-532-3p/CCR7

Xue

Liu

Nie³

et al. 2022

Disease Markers

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Background. lncRNAs have been indicated to involve in cell invasion, proliferation, and metastasis. However, function of DARS-AS1 in osteosarcoma remains poorly explored. Methods. DARS-AS1 and miR-532-3p level were measured using qRT-PCR. CCK-8 assay and cell invasion assay were done to study cell functions. Luciferase reporter assay was performed to study the mechanism about DARS-AS1 and miR-532-3p. Results. We firstly showed that DARS-AS1 expression is upregulated in 73.5% (25/34) of cases with osteosarcoma. Moreover, DARS-AS1 expression is overexpressed in osteosarcoma specimens than in nontumor samples. The DARS-AS1 is overexpressed in the osteosarcoma cell lines (Saos-2, SOSP-9607, U2OS, and MG-63) compared to hFOB. Overexpression of DARS-AS1 promotes cell growth and invasion in MG-63 osteosarcoma cell. DARS-AS1 plays as one sponge for miR-532-3p in osteosarcoma cell, and miR-532-3p overexpression inhibits luciferase activity of DARS-AS1-WT, not DARS-AS1-MUT in MG-63 cell. Ectopic expression of DARS-AS1 inhibits miR-532-3p expression in MG-63 cell. Furthermore, miR-532-3p expression is downregulated in osteosarcoma specimens compared to in paired nontumor samples. MiR-532-3p expression is downregulated in osteosarcoma cell lines compared to hFOB. MiR-532-3p expression is negatively associated with DARS-AS1 expression in osteosarcoma specimens. miR-532-3p directly regulates CCR7 expression in osteosarcoma cell. Elevated DARS-AS1 expression enhances cell growth and invasion via regulating CCR7. Conclusions. These data firstly suggested that DARS-AS1 exerted as one oncogene in osteosarcoma partly via regulating miR-532-3p/CCR7.

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Upregulation of lncRNA DARS‐AS1 accelerates tumor malignancy in cervical cancer by activating cGMP‐PKG pathway

Kong

Wang

Yang

2021

J Biochem & Molecular Tox

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This paper investigates the function of lncRNA DARS-AS1 in cervical cancer (CC) as well as its in-depth mechanism. The differential expression of DARS-AS1 and ATP1B2 were analyzed based on The Cancer Genome Atlas and the Genotype-Tissue Expression databases, and the survival rate was measured using Kaplan-Meier survival analysis. Biological function experiments were performed to detect cell proliferation, invasion, and migration. Quantitative real-time polymerase chain reaction was carried out to detect the expression of DARS-AS1 and ATP1B2. Western blot analysis was utilized to assess the protein levels of ATP1B2 and cGMP-PKG pathway-related proteins. DARS-AS1 was expressed at high levels in CC tissues and cell lines, and high expression of DARS-AS1 indicated a lower survival rate. CCK-8 and colony formation assays revealed that the overexpression of DARS-AS1 promoted the proliferation of CC cells. Furthermore, bioinformatics analysis suggested that the cGMP-PKG pathway ranks as the first pathway enriched by the differential genes that correlated with DARS-AS1 (|r| > 0.4). ATP1B2, as a cGMP-PKG pathway-related gene, was significantly correlated with the overall survival of CC patients. We further confirmed that ATP1B2 was lowly expressed in CC and negatively correlated with the DARS-AS1 expression. Then, biological function experiments exhibited that the promotion of cell proliferation, invasion, and migration resulted due to the upregulation of DARS-AS1 could be canceled by ATP1B2 overexpression. Finally, Western blot revealed that upregulation of DARS-AS1 could activate the cGMP-PKG pathway, while overexpression of ATP1B2 reversed this activation. Our study revealed that DARS-AS1/ATP1B2 contributes to regulating the progression of CC at least partially by modulating the cGMP-PKG pathway.

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Long non-coding RNA DARS-AS1 promotes tumorigenesis of non-small cell lung cancer via targeting miR-532-3p

Cited by 19 publications

References 4 publications

LncRNA DARS-AS1 aggravates the growth and metastasis of hepatocellular carcinoma via regulating the miR-3200-5p-Cytoskeleton associated protein 2 (CKAP2) axis

LncRNA DARS-AS1 aggravates the growth and metastasis of hepatocellular carcinoma via regulating the miR-3200-5p-Cytoskeleton associated protein 2 (CKAP2) axis

lncRNA DARS-AS1 Promoted Osteosarcoma Progression through Regulating miR-532-3p/CCR7

Upregulation of lncRNA DARS‐AS1 accelerates tumor malignancy in cervical cancer by activating cGMP‐PKG pathway

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