Long non-coding RNA DANCR facilitates glioma malignancy by sponging miR-33a-5p

Abstract

Glioma is among the most fatal brain tumors characterized by a highly malignancy and rapid progression and early metastasis. Dysregulation of long non-coding RNA differentiation antagonizing non-protein coding RNA (LncRNA DANCR) is associated with the development, progression and metastasis of various cancers. In the present study, we investigated functional role of LncRNA DANCR in the malignancy of glioma. The results showed that LncRNA DANCR was increased in glioma tissues and cells compared with normal brain tissues and cells. DANCR expression was positively correlated with the malignancy and poor prognosis of glioma patients. DANCR contained a binding site of miR-33a-5p. miR-33a-5p was decreased in glioma tissues and cells compared with normal brain tissues and cells. Downregulation of miR-33a-5p was positively correlated with the malignancy and poor prognosis of glioma patients. In glioma tissues, the expression of DANCR was negatively correlated with the expression of miR-33a-5p. Downregulation of DANCR increased miR-33a-5p expression. miR-33a-5p mimic reduced the luciferase of DANCR-WT but not DANCR-MUT. DANCR pull-down showed the expression of miR-33a-5p. miR-33a-5p mimic enhanced knockdown of DANCR -induced inhibition of cell proliferation, migration, and EMT, and increase of apoptosis. Anti-miR-33a-5p reversed the effects of si- DANCR on cell malignancy. Knockdown of DANCR remarkably reduced the increase of tumor volumes in xenograft mouse models. In tumor tissues, knockdown of DANCR increased the expression of miR-33a-5p, reduced EMT and increased apoptosis. Our study provides novel insights in the functions of LncRNA DANCR-miR-33a-5p axis in tumorigenesis of glioma.

Long noncoding RNA DANCR confers cytarabine resistance in acute myeloid leukemia by activating autophagy via the miR‐874‐3P/ATG16L1 axis

“…One critical mechanism by which lncRNAs regulate gene expression is acting as ceRNAs to sponge targeted miRNAs (Salmena et al, 2011). This is also true for DANCR, not only because several miRNAs have been identified as direct targets of DANCR, but also the function of DANCR is prominently mediated through downregulating the expression of these miRNAs, as reported by recent studies Lu et al, 2018;Wang et al, 2018a;Wang et al, 2018b;Xu et al, 2018;Yang et al, 2018), to name a few. Combining predictive methods and some biochemical experiments, we demonstrate that miR-874-3P is a new binding target of DANCR and that by suppressing the inhibitory effect of miR-874-3P on ATG16L1 expression via interacting and decreasing miR-874-3P level, DANCR possesses an ability to upregulate ATG16L1 expression.…”

DANCR‐mediated microRNA‐665 regulates proliferation and metastasis of cervical cancer through the ERK/SMAD pathway

“…Furthermore, Guo et al reported that lncRNA SNHG20 promotes cell proliferation and invasion by the miR‐140‐5p‐ADAM10 axis in cervical cancer. Recently, many studies have reported that lnc‐DANCR plays an important role in esophageal cancer, glioma, osteosarcoma, colorectal cancer, cervical cancer, and so on. Recently, Liang et al reported that lnc‐DANCR promoted cervical cancer progression by upregulating ROCK1 through sponging miR‐335‐5p .…”

Long noncoding RNA DANCR knockdown inhibits proliferation, migration and invasion of glioma by regulating miR-135a-5p/BMI1

“…For instance, DANCR regulated RAB1A expression in glioma by functioning as a ceRNA of miR-634 [27]. In addition, DANCR has been reported to promote glioma malignancy by miR-33a-5p [28]. However, there are no more reports on target miRNAs of DANCR in glioma.…”