Long non‑coding RNA CCAT1 enhances human non‑small cell lung cancer growth through downregulation of microRNA‑218

Zhao, Lei; Wang, Limin; Wang, Yongfeng; Ma, Ping

doi:10.3892/or.2020.7500

Cited by 6 publications

(8 citation statements)

References 27 publications

(23 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…25 In consistency with the previous finding, we also observed significantly higher CCAT1 expression in NSCLC cells than in human bronchial epithelial cell 16HBE. 8 Actually, CCAT1 was located around the proto-oncogene c-Myc, thus possibly contributing to upregulate its expression. 26 Similarly, the expression level of CCAT1 in radioresistant breast cancer tissues was remarkably higher than that in radiosensitive breast cancer tissues.…”

Section: Effects Of Ccat1 On the Radiosensitivity Of Nsclc Cells May Be Related To Mapk Signaling Pathwaymentioning

confidence: 99%

“…For example, CCAT1 knockdown in prostate cancer can hinder the survival and reduce the half maximal inhibitory concentration (IC50) of paclitaxel (PTX), but enhance the apoptosis rate of PC3-TXR and DU145-TXR cells treated with PTX. 28 Of note, CCAT1 gene deletion can induce the apoptosis of human NSCLC cells, 8 and further increase the apoptosis of NSCLC cells induced by gefitinib. 20 On the other hand, it has also been shown a correlation of the radiosensitivity of tumor cells with cell cycle.…”

Section: Effects Of Ccat1 On the Radiosensitivity Of Nsclc Cells May Be Related To Mapk Signaling Pathwaymentioning

confidence: 99%

“…Long noncoding RNA (lncRNA) is a newly found RNA family consisting of over 200 nucleotides, and cannot encode proteins due to the lack of complete open reading framework. 8 In recent years, many studies have reported the effects of lncRNAs on the radiotherapy of lung cancer, including SBF2-AS1, 9 GAS5, 10 PVT1, 11 and CRNDE. 12 LncRNA CARLo-5 was initially found abnormally elevated in colon cancer with very high specificity and sensitivity, demonstrating a relation with invasion, metastasis and prognosis of colon cancer, thus it is also named as colon cancer associated transcript-1 (CCAT1).…”

mentioning

confidence: 99%

See 2 more Smart Citations

LncRNA CCAT1 downregulation increases the radiosensitivity of non‐small cell lung cancer cells

Zhao

2021

The Kaohsiung J of Med Scie

View full text Add to dashboard Cite

This study aims to investigate if the radiosensitivity of non-small cell lung cancer (NSCLC) cells can be regulated by long noncoding RNA (lncRNA) colon cancer associated transcript1 (CCAT1). CCAT1 was detected by quantitative reverse transcriptionpolymerase chain reaction (qRT-PCR) in NSCLC cells (A549, H1299, SK-MES1, H460, and H647) and human bronchial epithelial cells (16HBE). H460 and A549 cells were then selected for the determination of CCAT1 expression after exposure to radiation (0, 2, 4, 6 Gy) at different time points (0, 6, 12, 24 h). Colony forming assay was performed to evaluate the effects of CCAT1 siRNA or pcDNA3.1-CCAT1 vector on the radiosensitivity of H460 and A549 cells. Then, flow cytometry, western blotting and qRT-PCR were also conducted. CCAT1 was increased in NSCLC cells when compared with 16HBE cells, which was declined in a time-and dosage-dependent manner after exposure to radiation. The H460 and A549 cell colonies were decreased and the γ-H2AX expression was elevated with the increase of radiation dosage, which was more obvious in those transfected with CCAT1 siRNA. CCAT1 downregulation arrested NSCLC cells at G2/M phase. Moreover, the enhanced apoptosis of radiotherapy-treated NSCLC cells with reductions of p-p38/p38, p-ERK/ ERK, and p-JNK/JNK was promoted by siCCAT1, but it was reversed by pcDNA3.1-CCAT1 vector. Inhibiting CCAT1 regulated cell cycle, DNA damage and apoptosis of NSCLC cells, and affected MAPK pathway, eventually improving the radiosensitivity of NSCLC.

show abstract

Section: Effects Of Ccat1 On the Radiosensitivity Of Nsclc Cells May Be Related To Mapk Signaling Pathwaymentioning

confidence: 99%

Section: Effects Of Ccat1 On the Radiosensitivity Of Nsclc Cells May Be Related To Mapk Signaling Pathwaymentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

LncRNA CCAT1 downregulation increases the radiosensitivity of non‐small cell lung cancer cells

Zhao

2021

The Kaohsiung J of Med Scie

View full text Add to dashboard Cite

show abstract

“…Chen et al identified the oncogene function of CCAT1 and induce chemoresistance in docetaxel resistant NSCLC cells 26 . Zhao et al shown that the upregulated CCAT1 in NSCLC cells was involved in the poor prognosis in NSCLC and knockdown of CCAT1 induces apoptosis and inhibits progression by targeting the miR‐218/BMI‐1 axis in NSCLC cells 27 …”

Section: Introductionmentioning

confidence: 99%

Long non‐coding RNAs as potential biomarkers in the prognosis and diagnosis of lung cancer: A review and target analysis

et al. 2020

View full text Add to dashboard Cite

Long non-coding RNAs (lncRNA) have been emerged as a novel class of molecular regulators in cancer. They are dysregulated in many types of cancer; however, there is not enough knowledge available on their expression and functional profiles. Lung cancer is the leading cause of the cancer deaths worldwide. Generally, lncRNAs may be associated with lung tumor pathogenesis and they may act as biomarkers for the cancer prognosis and diagnosis. Compared to other invasive prognostic and diagnostic methods, detection of lncRNAs might be a user-friendly and noninvasive method. In this review article, we selected 27 tumor-associated lncRNAs by literature reviewing to further discussing in detail for using as diagnostic and prognostic biomarkers in lung cancer. Also, in an in silico target analysis, the "Experimentally supported functional regulation" approach of the LncTarD web tool was used to identifying the target genes and regulatory mechanisms of the selected lncRNAs. The reports on diagnostic and prognostic potential of all selected lncRNAs were discussed. However, the target genes and regulatory mechanisms of the 22 lncRNAs were identified by in silico analysis and we found the pathways that are controlled by each target group of lncRNAs. They use epigenetic mechanisms, ceRNA mechanisms, protein interaction and sponge mechanism. Also, 10, 23, 5, and 28 target genes for each of these mechanisms were identified, respectively. Finally, each group of target genes controls 50, 12, 7, and 2 molecular pathways, respectively. In conclusion, LncRNAs could be used as

show abstract

“…Zhao's study found that lncRNA-CCAT1 knockdown suppressed tumor proliferation and induced apoptosis. High expression of lncRNA-CCAT1 was related to tumor growth and reduced survival rate, which can be a novel marker for lung cancer[29]. Ju's study systematically found prognosis-related lncRNAs, miRNAs, and mRNAs and constructed a prognosis-related competing endogenous RNA network[30].…”

mentioning

confidence: 99%

Large-Scale Transcriptome Analysis Identified Novel Ferroptosis-Related Gene Signatures for Predicting the Prognostic of Patients with Lung Adenocarcinoma

Sun

Liu

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Lung cancer is the most common of all malignant tumors. Traditional tumor staging has general sensitivity and specificity in predicting patient prognosis. Ferroptosis is a novel form of non-apoptotic form of cell death promoted by lipid peroxidation. Ferroptosis may be involved in the malignant progression of tumors through the regulation of glutathione depletion or the P53 signaling pathway. However, there are fewer studies on the impact of ferroptosis on tumor prognosis.Methods: We summarized 22 molecules that regulate ferroptosis. The transcriptome and corresponding clinical data were obtained from the Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) databases. The Wilcoxon test was utilized to analyze the expression of ferroptosis-related genes. We established risk score signatures, and all patients divided into two groups. Survival curves and multifactorial Cox regression analyses were performed to explore the prognostic value of ferroptosis on lung adenocarcinoma (LUAD).Results: We found that most ferroptosis-related genes are specifically expressed in LUAD tissue. We established a six‑mRNA signature and a seven‑IncRNA signature. All the models showed high predictive performance (AUC: 0.66–0.8), and patients in the low-risk group had higher overall survival than those in the high-risk group (P<0.05). The risk score is an independent risk factor for predicting the prognosis of LUAD.Conclusions: Our study demonstrates the critical role of ferroptosis in LUAD, and it is expected to supply a reference for the prognostic stratification of LUAD.

show abstract

Long non‑coding RNA CCAT1 enhances human non‑small cell lung cancer growth through downregulation of microRNA‑218

Cited by 6 publications

References 27 publications

LncRNA CCAT1 downregulation increases the radiosensitivity of non‐small cell lung cancer cells

LncRNA CCAT1 downregulation increases the radiosensitivity of non‐small cell lung cancer cells

Long non‐coding RNAs as potential biomarkers in the prognosis and diagnosis of lung cancer: A review and target analysis

Large-Scale Transcriptome Analysis Identified Novel Ferroptosis-Related Gene Signatures for Predicting the Prognostic of Patients with Lung Adenocarcinoma

Contact Info

Product

Resources

About