Long non-coding RNA CASC2 restrains high glucose-induced proliferation, inflammation and fibrosis in human glomerular mesangial cells through mediating miR-135a-5p/TIMP3 axis and JNK signaling

Dong-ju, Zhu; Wu, Xiang; Xue, Qingwu

doi:10.1186/s13098-021-00709-5

Cited by 12 publications

(11 citation statements)

References 35 publications

(40 reference statements)

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“…The uncontrolled proliferation of renal mesangial cells is an important pathological feature of human renal diseases, including diabetic nephropathy (Cove‐Smith & Hendry, 2008). In our study, we found that the proliferation of HRMCs was promoted under HG condition, which was consistent with the results of previous studies (Wang et al, 2022; Zhu et al, 2021). Hyperglycemia‐induced oxidative stress has important roles in pathogenesis of diabetic nephropathy (Rosas‐Martínez et al, 2021; Vodosek Hojs et al, 2020), and HG could result in oxidative stress in renal mesangial cells (Wan et al, 2022; Wei et al, 2021).…”

Section: Discussionsupporting

confidence: 93%

Salviolone protects against high glucose‐induced proliferation, oxidative stress, inflammation, and fibrosis of human renal mesangial cells by upregulating membrane metalloendopeptidase expression

Wang

Ruili

2022

Chem Biol Drug Des

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As one of complications of diabetes mellitus, diabetic nephropathy is related to renal dysfunction. Membrane metalloendopeptidase (MME) is associated with the pathogenesis of diabetic nephropathy and exerts a protective function in high glucose (HG)‐treated podocytes. Salviolone, one of important bioactive components from Salvia miltiorrhiza, possesses an anti‐inflammatory activity. However, the roles of salviolone in renal mesangial cell dysfunction under HG condition remain unknown. The targets of salviolone in diabetic nephropathy were predicted by bioinformatics analysis. Relative mRNA level of MME was detected by qPCR in HG‐treated human renal mesangial cells (HRMCs). Cell viability was analyzed using CCK‐8 assay. Cell proliferation was investigated by EdU staining. Oxidative stress was evaluated by detection of ROS generation and levels of oxidative stress‐related biomarkers. The inflammatory cytokines and fibrosis‐related biomarkers were examined by ELISA. Our results showed that MME expression was decreased in diabetic nephropathy and HG‐treated HRMCs. Salviolone increased MME level in HG‐treated HRMCs. Salviolone mitigated HG‐induced HRMC proliferation by increasing MME expression. Salviolone attenuated HG‐induced ROS generation, MDA level increase, and SOD activity decrease through upregulating MME expression. Moreover, salviolone suppressed HG‐induced increase of levels of TNF‐α, IL‐1β, IL‐6, fibronectin, and collagen IV through upregulating MME expression. In conclusion, salviolone attenuates proliferation, oxidative stress, inflammation, and fibrosis in HG‐treated HRMCs through upregulating MME expression.

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Section: Discussionsupporting

confidence: 93%

Salviolone protects against high glucose‐induced proliferation, oxidative stress, inflammation, and fibrosis of human renal mesangial cells by upregulating membrane metalloendopeptidase expression

Wang

Ruili

2022

Chem Biol Drug Des

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“…It is generally accepted that lncRNAs exert their biological roles by acting as miRNA sponges and forming an lncRNA-miRNA-mRNA axis. 29 SNHG6 sponges miR-944 and promotes KPNA5 expression, thereby weakening gemcitabine resistance in pancreatic cancer. 30 In addition, SNHG6 enhances hyperoxia-induced lung cell injury via miR-335-KLF5.…”

Section: Discussionmentioning

confidence: 99%

Suppression of long noncoding RNA SNHG6 alleviates cigarette smoke‐induced lung inflammation by modulating NF‐κB signaling

Yang,

Yuan,

Wang

et al. 2024

Environmental Toxicology

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BackgroundChronic obstructive pulmonary disease (COPD) is a widespread inflammatory disease with a high mortality rate. Long noncoding RNAs play important roles in pulmonary diseases and are potential targets for inflammation intervention.MethodsThe expression of small nucleolar RNA host gene 6 (SNHG6) in mouse lung epithelial cell line MLE12 with or without cigarette smoke extract (CSE) treatment was first detected using quantitative reverse‐transcription PCR. ELISA was used to evaluate the release of inflammatory cytokines (TNF‐α, IL‐1β, and IL‐6). The binding site of miR‐182‐5p with SNHG6 was predicted by using miRanda, which was verified by double luciferase reporter assay.ResultsHere, we revealed that SNHG6 was upregulated in CS‐exposed MLE12 alveolar epithelial cells and lungs from COPD‐model mice. SNHG6 silencing weakened CS‐induced inflammation in MLE12 cells and mouse lungs. Mechanistic investigations revealed that SNHG6 could upregulate IκBα kinase through sponging the microRNA miR‐182‐5p, followed by activated NF‐κB signaling. The suppressive effects of SNHG6 silencing on CS‐induced inflammation were blocked by an miR‐182‐5p inhibitor.ConclusionOverall, our findings suggested that SNHG6 regulates CS‐induced inflammation in COPD by activating NF‐κB signaling, thereby offering a novel potential target for COPD treatment.

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“…Previously, miR‐135a‐5p was mainly discussed in the context of functional disability in cancer, neurogenic diseases and cardiovascular diseases 17–19 . However, miR‐135a‐5p may also contribute to inflammation, adipogenesis and glucose metabolism, implying its potential for further mechanical exploration of GDM 20–22 . It is still unknown how miR‐135a‐5p affects the development of GDM.…”

Section: Discussionmentioning

confidence: 99%

Placenta‐derived exosomal miR‐135a‐5p promotes gestational diabetes mellitus pathogenesis by activating PI3K/AKT signalling pathway via SIRT1

Zhang,

Ye,

et al. 2023

J Cellular Molecular Medi

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Most people are aware of gestational diabetes mellitus (GDM), a dangerous pregnancy complication in which pregnant women who have never been diagnosed with diabetes develop chronic hyperglycaemia. Exosomal microRNA (miRNA) dysregulation has been shown to be a key player in the pathophysiology of GDM. In this study, we looked into how placental exosomes and their miRNAs may contribute to GDM. When compared to exosomes from healthy pregnant women, it was discovered that miR‐135a‐5p was elevated in placenta‐derived exosomes that were isolated from the maternal peripheral plasma of GDM women. Additionally, we discovered that miR‐135a‐5p encouraged HTR‐8/SVneo cell growth, invasion and migration. Further research revealed that miR‐135a‐5p activates HTR‐8/SVneo cells' proliferation, invasion and migration by promoting PI3K/AKT pathway activity via Sirtuin 1 (SIRT1). The transfer of exosomal miR‐135a‐5p generated from the placenta could be viewed as a promising agent for targeting genes and pertinent pathways involved in GDM, according to our findings.

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Long non-coding RNA CASC2 restrains high glucose-induced proliferation, inflammation and fibrosis in human glomerular mesangial cells through mediating miR-135a-5p/TIMP3 axis and JNK signaling

Cited by 12 publications

References 35 publications

Salviolone protects against high glucose‐induced proliferation, oxidative stress, inflammation, and fibrosis of human renal mesangial cells by upregulating membrane metalloendopeptidase expression

Salviolone protects against high glucose‐induced proliferation, oxidative stress, inflammation, and fibrosis of human renal mesangial cells by upregulating membrane metalloendopeptidase expression

Suppression of long noncoding RNA SNHG6 alleviates cigarette smoke‐induced lung inflammation by modulating NF‐κB signaling

Placenta‐derived exosomal miR‐135a‐5p promotes gestational diabetes mellitus pathogenesis by activating PI3K/AKT signalling pathway via SIRT1

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