Long non-coding RNA ANRIL promotes proliferation, clonogenicity, invasion and migration of laryngeal squamous cell carcinoma by regulating miR-181a/Snai2 axis

Yu, Hao; Zhang, Dejun; Fu, Zhongying; Guo, Yingyuan; Guan, Guofang

doi:10.1016/j.reth.2019.07.007

Cited by 25 publications

(19 citation statements)

References 35 publications

(34 reference statements)

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“…Due to higher expression levels of lncRNA ANRIL in Daudi and CA46 cells, the cell lines Daudi and CA46 were selected for further study. The present results revealed that the genetic silencing of lncRNA ANRIL inhibited cell proliferation and enhanced cell apoptosis, as evidenced by decreased cell viability and downregulated Ki67 expression levels, and increased levels of apoptotic cells and Hoechst-positive cells; these findings are consistent with those reported in laryngeal squamous cell (29) and hepatocellular carcinoma (30). The cell cycle is a fundamental process for cell life that serves an essential role in the accurate regulation of the survival, reproduction, development and inheritance of an organism.…”

Section: Discussionsupporting

confidence: 91%

Knockdown of long non‑coding RNA ANRIL inhibits the proliferation and promotes the apoptosis of Burkitt lymphoma cells through the TGF‑β1 signaling pathway

Mao

Jin

et al. 2020

Mol Med Rep

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Burkitt lymphoma (BL) has a high mortality rate and its treatment is currently limited to chemotherapy combined with immunotherapy. The long non-coding RNA antisense non-coding RNA in the INK4 locus (ANRIL) has been identified as an oncogene that can regulate cell proliferation and apoptosis in multiple types of cancer. However, the function of ANRIL in BL remains unknown. The present study aimed to determine the effect of ANRIL on cell proliferation and apoptosis in BL. Reverse transcription-quantitative PCR was used to analyze the expression levels of ANRIL in BL cells. The effect of ANRIL knockdown on BL cells was determined using Cell Counting Kit-8, flow cytometric, western blotting, immunofluorescence staining and Hoechst staining assays. The results revealed that ANRIL silencing inhibited the proliferation and promoted the apoptosis of BL cells. In addition, the expression levels of cyclin D1, E2F transcription factor 1 and Bcl-2 were downregulated, while the expression levels of cyclin-dependent kinase inhibitor 1A, Bcl-2-associated X protein, cleaved-caspase-9/pro-caspase-9 and cleaved-caspase-3/pro-caspase-3 were upregulated. Furthermore, the knockdown of ANRIL activated the TGF-β1 signaling pathway, as evidenced by the upregulated expression levels of TGF-β1, phosphorylated (p)-SMAD2/3/SMAD2/3, p-SMAD1/SMAD1 and sphingosine-1-phosphate receptor 2. Moreover, the protective effect of ANRIL silencing in BL could be inhibited by the TGF-β receptor type I/II dual inhibitor, LY2109761. In conclusion, the findings of the present study suggested that the knockdown of ANRIL may inhibit cell proliferation and promote cell apoptosis in BL by regulating the TGF-β1 signaling pathway, which may provide a novel target for the treatment of BL.

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Section: Discussionsupporting

confidence: 91%

Knockdown of long non‑coding RNA ANRIL inhibits the proliferation and promotes the apoptosis of Burkitt lymphoma cells through the TGF‑β1 signaling pathway

Mao

Jin

et al. 2020

Mol Med Rep

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“…Long non-coding RNA (lncRNA) antisense non-coding RNA in the INK4 locus (ANRIL) is located in the chromosome 9p21 region and is identified in patients with familial melanoma with germline deletion in the INK4B-ARF-INK4A gene cluster (5,6). Several studies have reported that lncRNA ANRIL is essential for the pathogenesis of various cancers (7)(8)(9)(10)(11)(12)(13). For instance, lncRNA ANRIL enhances the growth, invasion and migration of cancer cells in laryngeal squamous cell and hepatocellular carcinoma (7,8).…”

Section: Introductionmentioning

confidence: 99%

“…Several studies have reported that lncRNA ANRIL is essential for the pathogenesis of various cancers (7)(8)(9)(10)(11)(12)(13). For instance, lncRNA ANRIL enhances the growth, invasion and migration of cancer cells in laryngeal squamous cell and hepatocellular carcinoma (7,8). In AML, lncRNA ANRIL facilitates cell proliferation, migration and invasion, and represses cell apoptosis by modulating the expression of microRNA (miR)-34a, histone deacetylase 1 and ASPP2 (12).…”

Section: Introductionmentioning

confidence: 99%

Long non‑coding RNA ANRIL is a potential indicator of disease progression and poor prognosis in acute myeloid leukemia

et al. 2020

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The present study explored the association of long non-coding RNA (lncRNA) antisense non-coding RNA in the INK4 locus (ANRIL) with the development of acute myeloid leukemia (AML) clinical features and prognosis of patients with AML. Bone marrow mononuclear cells (BMMCs) were obtained from 178 patients with de novo AML prior to initial therapy and from 30 healthy donors. The expression of lncRNA ANRIL in BMMCs was detected by reverse transcription-quantitative PCR. Complete remission (CR) was assessed after induction therapy. Event-free survival (EFS) and overall survival (OS) were evaluated during the follow-up. The levels of lncRNA ANRIL were increased in patients with AML compared with those in healthy donors and were capable of distinguishing patients with AML from healthy donors (area under the curve, 0.886; 95% CI, 0.820-0.952). Furthermore, lncRNA ANRIL was associated with an increased occurrence internal tandem duplications in the FMS-like tyrosine kinase 3, decreased occurrence inv(16) or t(16;6), intermediate-risk and poor-risk stratification while no association of lncRNA ANRIL was identified with French-American-British classification, cytogenetics, isolated biallelic CCAAT/enhancer-binding protein α mutation and nucleophosmin 1 mutation in patients with AML. Furthermore, lncRNA ANRIL was significantly associated with a lower CR rate. In addition, EFS and OS were shorter in patients with high expression of lncRNA ANRIL compared with those in patients with low expression of lncRNA ANRIL. Multivariate Cox regression analyses revealed that high expression of lncRNA ANRIL, poor-risk stratification and white blood cells (>10.0x10 9 cells/l) were independent prognostic factors for shorter EFS, while high expression of lncRNA ANRIL and poorer risk stratification were independent prognostic factors for shorter OS. The present results suggested that lncRNA ANRIL has clinical relevance as a biomarker for assisting diagnosis treatment decisions and prognosis prediction and the identification of potential drug target for AML.

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“…The experimental results reported that ANRIL can directly combine with miR-181a sponge to counteract the inhibitory effect of miR-181a on Snail2 and play a positive role in regulating Snail2. Knockout of ANRIL gene or overexpression of miR-181a can greatly inhibit the proliferation, clonogenicity, invasion, migration, and EMT process of LSCC cells and elevate apoptosis (Hao et al, 2019).…”

Section: Lncrna and Emt Processmentioning

confidence: 99%

Regulatory Mechanisms of lncRNAs and Their Target Gene Signaling Pathways in Laryngeal Squamous Cell Carcinoma

Chen

Nie

2020

Front. Pharmacol.

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Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumor that occurs in the head and neck. People living in areas with serious air pollution and those who smoke and drink for a long time belong to high-risk groups. Although great progress has been made in chemotherapy, radiotherapy, and molecular targeted therapy in recent years, the prognosis of patients is still not good. The proliferation, invasion, and apoptosis of LSCC are controlled by many factors, which are the key factors influencing the prognosis of patients. Previous researches have demonstrated that long noncoding RNAs (lncRNAs) can be used as oncogenes or tumor suppressor genes in the occurrence and development of cancer and regulate cancer through various ways including epigenetic regulation and post-transcriptional regulation. The characteristics and roles of lncRNAs in LSCC, however, are not clear. In this review, we will discuss the role and function of lncRNAs in the proliferation, invasion, and apoptosis of LSCC and analyze the relationship between lncRNAs and lncRNA-regulated signaling pathways in LSCC pathological process. The difficulties faced by the related research of LSCC are discussed. It provides reference ideas for the molecular mechanism research of LSCC targeting lncRNA and its signaling pathways, the development of clinical prevention and therapeutic drug and individualized treatment, thereby improving the quality of life of patients.

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Long non-coding RNA ANRIL promotes proliferation, clonogenicity, invasion and migration of laryngeal squamous cell carcinoma by regulating miR-181a/Snai2 axis

Cited by 25 publications

References 35 publications

Knockdown of long non‑coding RNA ANRIL inhibits the proliferation and promotes the apoptosis of Burkitt lymphoma cells through the TGF‑β1 signaling pathway

Knockdown of long non‑coding RNA ANRIL inhibits the proliferation and promotes the apoptosis of Burkitt lymphoma cells through the TGF‑β1 signaling pathway

Long non‑coding RNA ANRIL is a potential indicator of disease progression and poor prognosis in acute myeloid leukemia

Regulatory Mechanisms of lncRNAs and Their Target Gene Signaling Pathways in Laryngeal Squamous Cell Carcinoma

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