Long non-coding RNA ANRIL-mediated inflammation response is involved in protective effect of rhein in uric acid nephropathy rats

Abstract

BackgroundThe aim of this study was to investigate the role of long non-coding RNAs (LncRNAs) antisense non-coding RNA in the INK4 locus (ANRIL) in anti-inflammation of rhein in uric acid nephropathy (UAN) rats.MethodsRat models of UAN were induced by adenine and potassium oxonate. Enzyme-linked immunosorbent assay (ELISA) was performed to assess inflammation factor in serum and supernatant. ANRIL mRNA level was detected using real-time reverse transcription PCR (qRT-PCR). Immunostaining was used to observe pathological changes of renal tissues in rats.ResultsANRIL and inflammatory factor levels were highly expressed in patient with UAN. Furthermore, rhein showed an observable effect on anti-inflammatory and renal protection in UAN rats, rhein inhibited expressions of ANRIL in vivo or in vitro. Besides, ANRIL-mediated inflammatory response attenuated protective effect of rhein.ConclusionsANRIL-mediated inflammatory response attenuated the protective effect of rhein in UAN rats. This study showed an understanding of the role and mechanism of ANRIL in UAN, which provides a new target and therapy for the prevention and treatment of UAN.Electronic supplementary materialThe online version of this article (10.1186/s13578-019-0273-3) contains supplementary material, which is available to authorized users.

Elevated circulating lnc‐ANRIL/miR‐125a axis level predicts higher risk, more severe disease condition, and worse prognosis of sepsis

“…These previous findings of lnc‐ANRIL suggest that lnc‐ANRIL might be a mediator of inflammatory and immune responses in various diseases. Thus, we hypothesized that it might be involved in the development of sepsis which is featured by greatly increased immune responses and inflammation, and we observed that lnc‐ANRIL expression in plasma had good predictive value for sepsis risk and was positively correlated with severity and pro‐inflammatory cytokines levels, which was probably derived from that lnc‐ANRIL could promote the progression of inflammation in sepsis through enhancing inflammatory responses or inflammasome expressions through mediating multiple signaling pathways, such as increasing NLRP3 inflammasome by regulating miR‐122‐5p/BRCC3 axis …”

“…Since its first discovery in melanoma, lnc‐ANRIL has been extensively studied, despite the fact that its role in oncology, the association of lnc‐ANRIL with inflammation and immunity has also been found recently . An in vitro and in vivo experiment shows that the inflammatory response mediated by lnc‐ANRIL markedly decreases the protective effect of rhein in rat models of uric acid nephropathy . Another experiment discloses that downregulating lnc‐ANRIL represses the progression of ulcerative colitis, an inflammatory disease, via mediating the miR‐323b‐5p/TLR4/MyD88/NF‐κB signaling pathway .…”

“…25 An in vitro and in vivo experiment shows that the inflammatory response mediated by lnc-ANRIL markedly decreases the protective effect of rhein in rat models of uric acid nephropathy. 13 Another experiment discloses that downregulating lnc-ANRIL represses the progression of ulcerative colitis, an inflammatory disease, via mediating the miR-323b-5p/TLR4/MyD88/ NF-κB signaling pathway. 26 In addition, an experiment that is also conducted in rat models of uric acid nephropathy reveals that lnc-ANRIL enhances the activation of the NLRP3 inflammasome by regulating the miR-122-5p/BRCC3 axis.…”

“…With respect to the values of noncoding RNAs to be biomarkers or therapeutic targets in sepsis patients, long noncoding RNA (lncRNA) and microRNA (miRNA) are the most implicated ones . Lnc‐antisense noncoding RNA in the INK4 locus (ANRIL) is a lncRNA located on chromosome 9p21 region and transcribed by RNA polymerase II and is indicated in the regulation of inflammation and immune responses . miR‐125a, a highly reserved miRNA locating on chromosome 19, 11, and 21, is also closely related to inflammation and immunity, and this miRNA has been reported to have the potential in regulating sepsis pathogenesis .…”

“…11,12 Lnc-antisense noncoding RNA in the INK4 locus (ANRIL) is a lncRNA located on chromosome 9p21 region and transcribed by RNA polymerase II and is indicated in the regulation of inflammation and immune responses. 13,14 miR-125a, a highly reserved miRNA locating on chromosome 19, 11, and 21, is also closely related to inflammation and immunity, and this miRNA has been reported to have the potential in regulating sepsis pathogenesis. 15,16 Moreover, it is a direct target of lnc-ANRIL, and the lnc-ANRIL/miR-125a axis has been indicated in many diseases.…”