Long non‑coding RNA 01614 hyperactivates WNT/β‑catenin signaling to promote pancreatic cancer progression by suppressing GSK‑3β

Chen, Long-Jiang; Wu, Lun; Wang, Wei; Zhai, Limin; Feng, Xin; Li, Weibo; Tang, Zhigang

doi:10.3892/ijo.2022.5406

Cited by 12 publications

(7 citation statements)

References 53 publications

(59 reference statements)

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“…Dysregulated phosphate metabolism enhances the osteoblast gene and promotes mineralization [ 48 ]. According to existing literature, LINC01614 can promote pancreatic cancer progression by WNT/β‑catenin signaling which is the crucial pathway in aortic valve calcification [ 49 ]. While expression of the WNT/β‑catenin signaling pathway can also be against phosphate-induced calcification [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Differential expression profiles and functional analysis of long non-coding RNAs in calcific aortic valve disease

Song

Guo

Yao

et al. 2023

BMC Cardiovasc Disord

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Aim To evaluate the expression profile of long non-coding RNAs (lncRNAs) in calcific aortic valve disease (CAVD) and explore their potential mechanism of action. Methods The gene expression profiles (GSE153555, GSE148219, GSE199718) were downloaded from the Gene Expression Omnibus (GEO) database and FastQC was run for quality control checks. After filtering and classifying candidate lncRNAs by differentially expressed genes (DEGs) and weighted co-expression networks (WGCNA) in GSE153555, we predicted the potential cis- or trans-regulatory target genes of differentially expressed lncRNAs (DELs) by using FEELnc and established the competitive endogenous RNA (ceRNA) network by miRanda, more over functional enrichment was analyzed using the ClusterProfiler package in R Bioconductor. The hub cis- or trans-regulatory genes were verified in GSE148219 and GSE199718 respectively. Results There were 340 up-regulated lncRNAs identified in AS group compared with the control group (|log2Fold Change| ≥ 1.0 and Padj ≤ 0.05), and 460 down-regulated lncRNAs. Based on target gene prediction and co-expression network construction, twelve Long non-coding RNAs (CDKN2B-AS1, AC244453.2, APCDD1L-DT, SLC12A5-AS1, TGFB3, AC243829.4, MIR4435-2HG, FAM225A, BHLHE40-AS1, LINC01614, AL356417.2, LINC01150) were identified as the hub cis- or trans-regulatory genes in the pathogenesis of CAVD which were validated in GSE148219 and GSE19971. Additionally, we found that MIR4435-2HG was the top hub trans-acting lncRNA which also plays a crucial role by ceRNA pattern. Conclusion LncRNAs may play an important role in CAVD and may provide a new perspective on the pathogenesis, diagnosis, and treatment of this disease. Further studies are required to illuminate the underlying mechanisms and provide potential therapeutic targets.

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Section: Discussionmentioning

confidence: 99%

Differential expression profiles and functional analysis of long non-coding RNAs in calcific aortic valve disease

Song

Guo

Yao

et al. 2023

BMC Cardiovasc Disord

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“…Wnt signaling pathway promotes tumor progression and metastasis in various types of cancer, such as gastric cancer, pancreatic cancer, lung cancer, and cervical cancer (24)(25)(26)(27)(28)(29). Previous studies have shown that the Wnt/β-catenin pathway is activated in numerous types of cancer, including cervical cancer (30-32).…”

Section: Discussionmentioning

confidence: 99%

OTX1 promotes tumorigenesis and progression of cervical cancer by regulating the Wnt signaling pathway

Zhou

Zhang

et al. 2022

Oncol Rep

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Cervical cancer is a common malignant tumor in females. Orthodenticle homolog 1 (OTX1) serves a key role in the occurrence and progression of tumors. The present study aimed to investigate the role and potential mechanism of OTX1 in cervical cancer. OTX1 expression was analyzed by western blotting, reverse transcription-quantitative PCR and immunohistochemistry. MTT assay was performed to assess cell viability. EdU and colony formation assay were used to measure cell proliferation. Wound healing and Transwell assays were performed to measure cell migration and invasion. Western blot assay was performed for the assessment of protein expression. Gene set enrichment analysis (GSEA) was performed to analyze signaling pathways regulated by OTX1. Co-Immunoprecipitation assay was performed to confirm the interaction between OTX1 and Wnt9b. In cervical cancer tissue and cells, OTX1 was significantly upregulated. OTX1 overexpression promoted proliferation, migration and invasion of cervical cancer cells. OTX1 silencing significantly decreased cell proliferation, migration and invasion of cervical cancer. GSEA showed that OTX1 activated the Wnt signaling pathway. OTX1 silencing inhibited the increased levels of adenomatous polyposis coli (APC), glycogen synthase kinase (GSK)-3β and axis inhibition protein (AXIN)2 and decreased levels of Wnt9b and β-catenin. OTX1 overexpression decreased the levels of APC, GSK-3β and AXIN2 and increased levels of Wnt9b and β-catenin. However, XAV939 (a Wnt signaling inhibitor) and β-catenin silencing partly eliminated the effect of OTX1 overexpression on cervical cancer cells. OTX1 promoted the progression of cervical cancer by activating the Wnt signaling pathway.

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“…Inhibiting the original activity of lncRNAs by binding specific proteins is another way to regulate the Wnt/β-catenin pathway. Both lncRNA long intergenic non-protein coding RNA 01614 (LINC01614) [35] binding GSK-3β and lncRNA LINC01197 [36] binding β-catenin can affect the original function of the protein to regulate Wnt signal transduction.…”

Section: Lncrnas Regulate Wnt/β-catenin Signaling Pathway In Pcmentioning

confidence: 99%

LncRNAs as nodes for the cross-talk between autophagy and Wnt signaling in pancreatic cancer drug resistance

Huang,

Zhang,

Lyu

et al. 2024

Int. J. Biol. Sci.

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Pancreatic cancer is a malignancy with high mortality. In addition to the few symptoms until the disease reaches an advanced stage, the high fatality rate is attributed to its rapid development, drug resistance and lack of appropriate treatment. In the selection and research of therapeutic drugs, gemcitabine is the first-line drug for pancreatic cancer. Solving the problem of gemcitabine resistance in pancreatic cancer will contribute to the progress of pancreatic cancer treatment. Long non coding RNAs (lncRNAs), which are RNA transcripts longer than 200 nucleotides, play vital roles in cellular physiological metabolic activities. Currently, our group and others have found that some lncRNAs are aberrantly expressed in pancreatic cancer cells, which can regulate the process of cancer through autophagy and Wnt/β-catenin pathways simultaneously and affect the sensitivity of cancer cells to therapeutic drugs. This review presents an overview of the recent evidence concerning the node of lncRNA for the cross-talk between autophagy and Wnt/β-catenin signaling in pancreatic cancer, together with the practicability of lncRNAs and the core regulatory factors as targets in therapeutic resistance.

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Long non‑coding RNA 01614 hyperactivates WNT/β‑catenin signaling to promote pancreatic cancer progression by suppressing GSK‑3β

Cited by 12 publications

References 53 publications

Differential expression profiles and functional analysis of long non-coding RNAs in calcific aortic valve disease

Differential expression profiles and functional analysis of long non-coding RNAs in calcific aortic valve disease

OTX1 promotes tumorigenesis and progression of cervical cancer by regulating the Wnt signaling pathway

LncRNAs as nodes for the cross-talk between autophagy and Wnt signaling in pancreatic cancer drug resistance

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