Long-lived protein radicals induced by X-ray irradiation are the source of reactive oxygen species in aqueous medium

Gudkov, Sergey V.; Гармаш, С. А.; Штаркман, И. Н.; Chernikov, A. V.; Karp, O. E.; Брусков, В. И.

doi:10.1134/s1607672910010011

Cited by 40 publications

(24 citation statements)

References 13 publications

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“…A DSB can potentially be caused upon action of these radicals on opposite strands. [37] Ward, ( 1981) [57] also reasoned that DSBs induced by high-LET radiation are an "attractive candidate" for investigating the production of BSS. However, he questioned the ability of one DSB being able to generate the BSS.…”

Section: Nuclear Dna Damage For Bystander Effect-to Be or Not To Be?mentioning

confidence: 97%

“…The traditional biological effects include multiple radicals reacting on opposite DNA strands. [37] Radiolytically generated OH-radicals form a radical with deoxyribose, but steric considerations make it unlikely to cause a break on the adjoining strand of DNA; hence single-strand breaks are more logical in this scenario. A DSB can potentially be caused upon action of these radicals on opposite strands.…”

Section: Nuclear Dna Damage For Bystander Effect-to Be or Not To Be?mentioning

confidence: 99%

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Radiation induced bystander effect and DNA damage

et al. 2014

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Bystander effects (BSEs) have been investigated for a long time but without much deliberation as to the cause in targeted cells and the subsequent effect in naïve cells. BSEs have traditionally been associated with radiation. Currently, this phenomenon is at a juncture where nuclear DNA damage is being debated as either essential or nonessential. If DNA damage is essential for the bystander signal (BSS) production then, this raises a number of questions about, radiotherapy and chemotherapy of cancer patients. This review presents a detailed analysis of the work done to investigate nuclear DNA damage versus exclusively cytoplasmic targeting with ionizing radiations and measurement of bystander end-points in naïve cells. The review also analyzes some of the research work done to investigate cell models that were developed specifically to study and track radiation-induced DNA damage to construct mutation spectra. Production of reactive oxygen species and reactive nitrogen species as possible candidates of the elusive BSS are also discussed besides the signal transduction pathways implicated in reception of a BSS by the naïve cell.

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Section: Nuclear Dna Damage For Bystander Effect-to Be or Not To Be?mentioning

confidence: 97%

Section: Nuclear Dna Damage For Bystander Effect-to Be or Not To Be?mentioning

confidence: 99%

Radiation induced bystander effect and DNA damage

et al. 2014

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“…Most patients diagnosed with head and neck cancer (HNC) in the US are treated with irradiation (IR) therapy alone, or IR in combination with surgery or chemotherapy. While IR is targeted to the tumor, damage to non-tumor adjacent tissues such as oral mucosa and the salivary glands also occurs, and in some cases can limit the course of therapy (1). This is despite improvements in radiation delivery such as intensity modulated radiation therapy (IMRT), which enable more precise delivery of IR to the tumor (2).…”

Section: Introductionmentioning

confidence: 99%

Tyrosine kinase inhibitors protect the salivary gland from radiation damage by increasing DNA double-strand break repair

Affandi

Ohm

Gaillard

et al. 2021

Journal of Biological Chemistry

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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“…Apparently, the radioprotective effect of Prdx6 is mediated by several components. First of all, by the peroxidase activity of Prdx6, which provides neutralization of a wide range of peroxide substrates, including long-lived reactive protein species (LRPS), Prdx6 eliminating LRPS more effectively than it was shown earlier for such compounds as inosine, guanosine, vitamin C, and l -methionine [116,117]. Secondly, it is caused also by the signaling and regulatory function of Prdx6, which facilitates triggering of recovery processes in stress conditions and is not related to its peroxidase activity [110,111].…”

Section: Application Of Exogenous Prdx6 As a Radioprotectormentioning

confidence: 99%

Radioprotective Role of Peroxiredoxin 6

2019

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Peroxiredoxin 6 (Prdx6) is a member of an evolutionary ancient family of peroxidase enzymes with diverse functions in the cell. Prdx6 is an important enzymatic antioxidant. It reduces a wide range of peroxide substrates in the cell, thus playing a leading role in the maintenance of the redox homeostasis in mammalian cells. Beside peroxidase activity, Prdx6 has been shown to possess an activity of phospholipase A2, an enzyme playing an important role in membrane phospholipid metabolism. Moreover, Prdx6 takes part in intercellular and intracellular signal transduction due to its peroxidase and phospholipase activity, thus facilitating the initiation of regenerative processes in the cell, suppression of apoptosis, and activation of cell proliferation. Being an effective and important antioxidant enzyme, Prdx6 plays an essential role in neutralizing oxidative stress caused by various factors, including action of ionizing radiation. Endogenous Prdx6 has been shown to possess a significant radioprotective potential in cellular and animal models. Moreover, intravenous infusion of recombinant Prdx6 to animals before irradiation at lethal or sublethal doses has shown its high radioprotective effect. Exogenous Prdx6 effectively alleviates the severeness of radiation lesions, providing normalization of the functional state of radiosensitive organs and tissues, and leads to a significant elevation of the survival rate of animals. Prdx6 can be considered as a potent and promising radioprotective agent for reducing the pathological effect of ionizing radiation on mammalian organisms. The radioprotective properties and mechanisms of radioprotective action of Prdx6 are discussed in the current review.

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Long-lived protein radicals induced by X-ray irradiation are the source of reactive oxygen species in aqueous medium

Cited by 40 publications

References 13 publications

Radiation induced bystander effect and DNA damage

Radiation induced bystander effect and DNA damage

Tyrosine kinase inhibitors protect the salivary gland from radiation damage by increasing DNA double-strand break repair

Radioprotective Role of Peroxiredoxin 6

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