Long-lived effector/central memory T-cell responses to severe acute respiratory syndrome coronavirus (SARS-CoV) S antigen in recovered SARS patients

Yang, Lei; Peng, Hui; Zeng, Zhu; Li, Gang; Huang, Zitong; Zhao, Zhigang; Koup, Richard A.; Bailer, Robert T.; Wu, Changyou

doi:10.1016/j.clim.2006.05.002

Cited by 118 publications

(141 citation statements)

References 26 publications

(30 reference statements)

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“…Of course, T cell responses are known to play their roles in protection against many viral infections and in supporting an anamnestic Ab response, because the anamnestic response of B cells depends on Agspecific memory CD4 + T cells for rapid reactivation and differentiation into Ab-secreting cells (14). Cellular immune responses to SARS-CoV were confirmed in both SARS patients and experimental animals (15,16), Memory T cell responses were detected in patients recovering from SARS 1, 2, and even 4 y later, although with a clear decline over time (17)(18)(19). Whether the memory T cell response will be maintained at a detectable level thereafter remains to be investigated.…”

Section: S Evere Acute Respiratory Syndrome (Sars) Is An Emerging Infmentioning

confidence: 89%

Lack of Peripheral Memory B Cell Responses in Recovered Patients with Severe Acute Respiratory Syndrome: A Six-Year Follow-Up Study

Tang

Yan

Xin

et al. 2011

The Journal of Immunology

505

468

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Six years have passed since the outbreak of severe acute respiratory syndrome (SARS). Previous studies indicated that specific Abs to SARS-related coronavirus (SARS-CoV) waned over time in recovered SARS patients. It is critical to find out whether a potential anamnestic response, as seen with other viral infections, exists to protect a person from reinfection in case of another SARS outbreak. Recovered SARS patients were followed up to 6 y to estimate the longevity of specific Ab. The specific memory B cell and T cell responses to SARS-CoV Ags were measured by means of ELISPOT assay. Factors in relation to humoral and cellular immunity were investigated. Six years postinfection, specific IgG Ab to SARS-CoV became undetectable in 21 of the 23 former patients. No SARS-CoV Ag-specific memory B cell response was detected in either 23 former SARS patients or 22 close contacts of SARS patients. Memory T cell responses to a pool of SARS-CoV S peptides were identified in 14 of 23 (60.9%) recovered SARS patients, whereas there was no such specific response in either close contacts or healthy controls. Patients with more severe clinical manifestations seemed to present a higher level of Ag-specific memory T cell response. SARS-specific IgG Ab may eventually vanish and peripheral memory B cell responses are undetectable in recovered SARS patients. In contrast, specific T cell anamnestic responses can be maintained for at least 6 y. These findings have applications in preparation for the possible reemergence of SARS.

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Section: S Evere Acute Respiratory Syndrome (Sars) Is An Emerging Infmentioning

confidence: 89%

Lack of Peripheral Memory B Cell Responses in Recovered Patients with Severe Acute Respiratory Syndrome: A Six-Year Follow-Up Study

Tang

Yan

Xin

et al. 2011

The Journal of Immunology

505

468

View full text Add to dashboard Cite

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“…Although whether the memory T-cell response is sufficient to protect from reinfection requires further investigation, it has been suggested that a more robust CTL response contributes to protection against SARS-CoV in mice Chen et al, 2010a;Zhao et al, 2010a). 2009; Peng et al, 2006;Yang et al, 2006Yang et al, , 2007. Unlike waning serum antibody levels in patients, CTL responses against the S and N proteins can still be detected from the PBMCs of recovered SARS patients 1, 2, 4, 6, and even >10 years post-infection (Da Guan et al, 2015;Ng et al, 2016;Oh et al, 2011;Tang et al, 2011).…”

Section: T-cell Immunity To Sars-covmentioning

confidence: 99%

T-cell immunity of SARS-CoV: Implications for vaccine development against MERS-CoV

et al. 2017

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Over 12 years have elapsed since severe acute respiratory syndrome (SARS) triggered the first global alert for coronavirus infections. Virus transmission in humans was quickly halted by public health measures and human infections of SARS coronavirus (SARS-CoV) have not been observed since. However, other coronaviruses still pose a continuous threat to human health, as exemplified by the recent emergence of Middle East respiratory syndrome (MERS) in humans. The work on SARS-CoV widens our knowledge on the epidemiology, pathophysiology and immunology of coronaviruses and may shed light on MERS coronavirus (MERS-CoV). It has been confirmed that T-cell immunity plays an important role in recovery from SARS-CoV infection. Herein, we summarize T-cell immunological studies of SARS-CoV and discuss the potential cross-reactivity of the SARS-CoV-specific immunity against MERS-CoV, which may provide useful recommendations for the development of broad-spectrum vaccines against coronavirus infections.

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“…And to respiratory syncytial virus (RSV) infection, CTLs not only contribute to virus elimination, but also induce immunopathology [15,16]. Excited to us, we found that SARS-CoV S specific CTL responses were detected in patients who had recovered from SARS and mouse model [17,18]. Therefore, CTLs may determine the role of immunity in controlling viral pathogenesis.…”

Section: Introductionmentioning

confidence: 97%

CD8+ T cell response in HLA-A*0201 transgenic mice is elicited by epitopes from SARS-CoV S protein

Zhao

Yang

et al. 2010

Vaccine

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Cytotoxic CD8(+) T lymphocytes (CTLs) play an important role in antiviral immunity. Several human HLA-A*0201 restricted CTL epitopes of severe acute respiratory syndrome (SARS) spike (S) protein have been identified in HLA-A*0201 transgenic (Tg) mice, but the mechanisms and properties of immune responses are still not well understood. In this study, HLA-A*0201 Tg mice were primed intramuscularly with SARS S DNA and boosted subcutaneously with HLA-A*0201 restricted peptides. The lymphocytes from draining lymph nodes, spleens and lungs were stimulated with the cognate peptides. Three different methods (ELISA, ELISPOT and FACS) were used to evaluate the immune responses during short and long periods of time after immunization. Results showed that peptide-specific CD8(+) T cells secreted IFN-γ, TNF-α and IL-2 and expressed CD107a/b on cell surface. IFN-γ(+)CD8(+) T cells and CD107a/b(+)CD8(+) T cells distributed throughout the lymphoid and non-lymphoid tissues, but the frequency of peptide-specific CD8(+) T cells was higher in lungs than in spleens and lymph nodes. The phenotype of the CD8(+) T cells was characterized based on the expression of IFN-γ. Most of the HLA-A*0201 restricted peptide-specific CD8(+) T cells represented a memory subset with CD45RB(high) and CD62L(low). Taken together, these data demonstrate that immunization with SARS S DNA and HLA-A*0201 restricted peptides can elicit antigen-specific CD8(+) T cell immune responses which may have a significant implication in the long-term protection. We provide novel information in cellular immune responses of SARS S antigen-specific CD8(+) T cells, which are important in the development of vaccine against SARS-CoV infection.

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Long-lived effector/central memory T-cell responses to severe acute respiratory syndrome coronavirus (SARS-CoV) S antigen in recovered SARS patients

Cited by 118 publications

References 26 publications

Lack of Peripheral Memory B Cell Responses in Recovered Patients with Severe Acute Respiratory Syndrome: A Six-Year Follow-Up Study

Lack of Peripheral Memory B Cell Responses in Recovered Patients with Severe Acute Respiratory Syndrome: A Six-Year Follow-Up Study

T-cell immunity of SARS-CoV: Implications for vaccine development against MERS-CoV

CD8+ T cell response in HLA-A*0201 transgenic mice is elicited by epitopes from SARS-CoV S protein

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