Long-chain Non-coding RNA MIR4435-2HG Expression Correlates With Size of Intestinal Polyps in Children and With Metastasis of Colon Cancer

Abstract

Background/Aim: To determine if long-chain noncoding RNA (lncRNA) MIR4435-2HG (MIR4435) expression is associated with pre-malignant colon polyps and colon cancer. Materials and Methods: Children's colonic-polyp specimens were sequenced for MIR4435 expression. LncRNA MIR4435 expression data in colorectal cancer and normal intestinal tissues were retrieved from The Cancer Genome Atlas (TCGA). The proliferation, adhesion, and invasion ability of human colon-cancer cell line HCT116 with or without MIR4435 knockdown was analyzed. The expression of Smad4, desmoplakin, and β-catenin genes was detected by western blotting in HCT116 cells. Results: MIR4435 expression correlated with the size of intestinal polyps in children. Expression of MIR4435 was up-regulated in colorectal cancer. MIR4435 knockdown in HCT116 cells inhibited their proliferation, adhesion, and invasion ability. Smad4 and desmoplakin were up-regulated and β-catenin was down-regulated in HCT116 cells by MIR4435 knockdown. Conclusion: MIR4435 expression correlated with the size of intestinal polyps in children and with the proliferation, adhesion, and invasion ability of colon-cancer cells and was upregulated in colon cancer.

Exploring potential biomarkers and therapeutic targets in inflammatory bowel disease: insights from a mega-analysis approach

“…In addition, it was shown that EZH2 suppression ameliorate intestinal inflammation and delay the onset of colitis associated cancer, suggesting the feasibility of EZH2 inhibitor for the control of IBD ( 74 ). Finally, the expression of MIR4435-2HG has been found to correlate with the size of intestinal polyps in Colorectal Cancer (CRC) ( 75 ) suggesting MIR4435-2HG to play a role in colorectal health. Collectively, these observations suggest that MIR4435-2HG may serve as a common molecular marker for inflammation-mediated processes and intestinal health.…”

Biological Roles and Pathogenic Mechanisms of LncRNA MIR4435-2HG in Cancer: A Comprehensive Review

“…The upregulation of MIR4435-2HG is closely linked to the size of tumors, the spread of cancer cells to lymph nodes, TNM staging, progression-free survival, and overall survival, indicating its potential as a biomarker for diagnosing and predicting colorectal cancer [11,[31][32][33]71]. The expression level of MIR4435-2HG is strongly associated with the ability of colon cancer cells to proliferate, adhere, and invade [34,35]. Inhibiting MIR4435-2HG can suppress the proliferation, invasion, and migration of colorectal cancer cells, as well as inhibit the growth of colorectal cancer and its spread to the liver in laboratory settings [11].…”