Long-chain monounsaturated fatty acids improve endothelial function with altering microbial flora

Tsutsumi, Rie; Yamasaki, Yuji; Takeo, Jiro; Miyahara, Hiroko; Sebe, Mayu; Bando, Masahiro; Tanba, Yousuke; Mishima, Yuna; Takeji, Kana; Ueshima, Nanako; Kuroda, Masashi; Masumoto, Shuji; Harada, Nagakatsu; Fukuda, Daiju; Yoshimoto, Ryoko; Tsutsumi, Yasuo M.; Aihara, Ken-ichi; Sata, Masataka; Sakaue, Hiroshi

doi:10.1016/j.trsl.2021.03.016

Cited by 31 publications

(23 citation statements)

References 62 publications

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“…The high content of LC MUFA (e.g., C20:1 n-9, gondoic acid and C22:1 n-11, cetoleic acid) recorded in this study is typical for oils from herring and mackerel (Yang et al, 2016 ), and are further to the LC n-3 PUFA themselves, interesting as a few animal studies have indicated they may prevent life-style related diseases, such as type 2 diabetes, metabolic syndrome, and atherosclerosis. For example, mice-studies have revealed that the mentioned LC MUFA can improve endothelial function by altering the microbial flora (Tsutsumi et al, 2021 ) as well as the glucose/lipid homeostasis (Yang et al, 2011 ). Cetoleic acid may also improve the efficiency of the conversion of ALA to EPA and DHA (Østbye et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Pilot-Scale Ensilaging of Herring Filleting Co-Products and Subsequent Separation of Fish Oil and Protein Hydrolysates

Sajib

Trigo

Abdollahi

et al. 2022

Food Bioprocess Technol

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In this study, ensilaging of herring (Clupea harengus) filleting co-products was taken from lab-scale to pilot scale (1500 L) while monitoring the protein degree of hydrolysis (DH) and lipid oxidation. Subsequently, the possibility of recovering fish oil and protein hydrolysates using batch centrifugation at different g-forces/times was investigated. Around 38% DH was recorded after 2-day pilot-scale ensilaging of herring co-products at ambient temperature (i.e., ~ 22 °C), which was similar to the DH found in lab-scale (40% after 2 days; 22 °C). The lipid oxidation marker 2-thiobarbituric acid reactive substances (TBARS) reached 20 µmole TBARS/kg silage after 2-day ensilaging. Centrifugation of the silage at 3000–8500 × g for 2–20 min revealed successful separation into fish oil and protein hydrolysates. Heat-treating the silage (85 °C; 30 min) prior to centrifugation resulted in significantly higher oil and hydrolysates recoveries; the same being true for increased g-force. At 8500 × g, the recovery of oil and hydrolysates were 9.7 and 53.0% w/w, respectively, from heat-treated silage, while recoveries were 4.1 and 48.1% w/w, respectively, from non-heat treated silage. At 4500 × g, being a more scalable approach, corresponding numbers were 8.2 and 47.1% (w/w) as well as 2.0 and 40.2% (w/w). The recovered fish oil contained 8% EPA and 11% DHA of total fatty acids. Free fatty acids (FFA), peroxide value (PV), p-anisidine value (p-AV), and total oxidation (TOTOX) values of oils were in the range of 4–7% (FFA), 3.6–3.7 meq/kg oil (PV), 2.5–4.0 (p-AV), and 9.9–11.1 (TOTOX), respectively, which were within the acceptable limits for human consumption specified by the GOED voluntary monograph. The recovered protein hydrolysates contained peptides in the molecular weight range 0.3–6 kDa (~ 37%) and 11–34 kDa (~ 63%). Also, the remaining solids contained 15–17% (w/w) protein, having 44–45% essential amino acids. Overall, the results suggest that herring co-product silage is a valuable source of fish oil and protein hydrolysates, paving the way for ensilaging based-biorefining of herring co-products into multiple products.

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Section: Resultsmentioning

confidence: 99%

Pilot-Scale Ensilaging of Herring Filleting Co-Products and Subsequent Separation of Fish Oil and Protein Hydrolysates

Sajib

Trigo

Abdollahi

et al. 2022

Food Bioprocess Technol

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“…Without any treatment, there was a higher baseline F/B ratio in male mice. A higher abundant Firmicutes and a lower abundant of Bacteroidetes are often manifestations of serious diseases [ 26 ]. In the experimental stroke model established in male mice, female microbiota transplantation transmitted the protective characteristics of females to males.…”

Section: Discussionmentioning

confidence: 99%

Different gender-derived gut microbiota influence stroke outcomes by mitigating inflammation

Wang

Zhong

Zhu

et al. 2022

J Neuroinflammation

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Background and purpose Stroke is associated with high disability and mortality rates and increases the incidence of organ-related complications. Research has revealed that the outcomes and prognosis of stroke are regulated by the state of the intestinal microbiota. However, the possibility that the manipulation of the intestinal microbiota can alter sex-related stroke outcomes remain unknown. Methods To verify the different effects of microbiota from different sexes on stroke outcomes, we performed mouse fecal microbiota transplantation (FMT) and established a model of ischemic stroke. Male and female mice received either male or female microbiota through FMT. Ischemic stroke was triggered by MCAO (middle cerebral artery occlusion), and sham surgery served as a control. Over the next few weeks, the mice underwent neurological evaluation and metabolite and inflammatory level detection, and we collected fecal samples for 16S ribosomal RNA analysis. Results We found that when the female mice were not treated with FMT, the microbiota (especially the Firmicutes-to-Bacteroidetes ratio) and the levels of three main metabolites tended to resemble those of male mice after experimental stroke, indicating that stroke can induce an ecological imbalance in the biological community. Through intragastric administration, the gut microbiota of male and female mice was altered to resemble that of the other sex. In general, in female mice after MCAO, the survival rate was increased, the infarct area was reduced, behavioral test performance was improved, the release of beneficial metabolites was promoted and the level of inflammation was mitigated. In contrast, mice that received male microbiota were much more hampered in terms of protection against brain damage and the recovery of neurological function. Conclusion A female-like biological community reduces the level of systemic proinflammatory cytokines after ischemic stroke. Poor stroke outcomes can be positively modulated following supplementation with female gut microbiota.

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“…The Ludwigshafen Risk and Cardiovascular Health Study showed an inverse association of gadoleic acid with LDL-C, HDL-C and eGFR but direct correlations with markers of inflammation and endothelial activation, as well as heart failure [ 60 ]. In contrast, a randomized controlled trial found that the supplementation of LCMUFAs (gadoleic acid and cetoleic acid) improved endothelial function by lowering trimethylamine-N-oxide levels, IL-6 and TNF-α, possibly due to improved gut microbiota profile [ 61 ]. The debate on the effects of LCMUFAs (more than 18 carbons) remains inconclusive as research is still scarce.…”

Section: Discussionmentioning

confidence: 99%

Dietary Fats and Cardio-Metabolic Outcomes in a Cohort of Italian Adults

et al. 2022

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Background: Dietary fats, and especially saturated fatty acid (SFA), have been blamed for being the culprit in the dramatic increase in obesity and its associated diseases. However multiple systematic reviews and recent meta-analyses do not support the association between SFA and cardiovascular diseases. Thus, the objective of this study was to test whether specific types and subtypes of dietary fats are associated with metabolic outcomes in a cohort of Italian adults. Methods: Nutritional and demographic data of 1936 adults living in the south of Italy were examined. Food frequency questionnaires (FFQs) were administered to assess the intake of total dietary fat and each specific class of dietary fat, such as SFA, monounsaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA). The intake of fatty acids was also examined according to the carbon-chain length of each individual class. Cases of hypertension, type-2 diabetes and dyslipidemias were collected from previous doctor-confirmed diagnosis records (or direct measurement of blood pressure). Results: After adjustment for potential confounding factors, individuals reporting higher intakes of total and saturated fats were associated with lower likelihood of having hypertension (odds ratio (OR) = 0.57, 95% CI: 0.35, 0.91 and OR = 0.55, 95% CI: 0.34, 0.89, respectively). Moreover, higher intake of short-chain saturated fatty acids (SCSFAs) and medium-chain saturated fatty acids (MCSFAs) was inversely associated with dyslipidemia and diabetes (OR = 0.43, 95% CI: 0.23, 0.82 and OR = 0.25, 95% CI: 0.09, 0.72, respectively). Among MUFAs, C18:1 was inversely associated with hypertension and diabetes (OR = 0.52, 95% CI: 0.30, 0.92 and OR = 0.21, 95% CI: 0.07, 0.67, respectively), while C14:1 intake was inversely associated only with hypertension (OR = 0.57, 95% CI: 0.37, 0.88). In contrast, C20:1 intake was associated with dyslipidemia (OR = 3.35, 95% CI: 1.33, 8.42). Regarding PUFA, C18:2 and 20:5 were inversely associated with hypertension (OR = 0.33, 95% CI: 0.18, 0.60 and OR = 0.30, 95% CI: 0.10, 0.89, respectively). Conclusions: The consumption of SFA does not seem to be harmful to cardio-metabolic health and, on the contrary, SCSFA may exert beneficial effects. Further studies are needed to clearly validate the results of the present study.

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Long-chain monounsaturated fatty acids improve endothelial function with altering microbial flora

Cited by 31 publications

References 62 publications

Pilot-Scale Ensilaging of Herring Filleting Co-Products and Subsequent Separation of Fish Oil and Protein Hydrolysates

Pilot-Scale Ensilaging of Herring Filleting Co-Products and Subsequent Separation of Fish Oil and Protein Hydrolysates

Different gender-derived gut microbiota influence stroke outcomes by mitigating inflammation

Dietary Fats and Cardio-Metabolic Outcomes in a Cohort of Italian Adults

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