Long-Chain Bases from Sea Cucumber Alleviate Obesity by Modulating Gut Microbiota

Hu, Shiwei; Xu, Yangli; Gao, Xiang; Li, Shijie; Jiang, Wei; Liu, Yu; Su, Laijin; Yang, Haitao

doi:10.3390/md17080455

Cited by 48 publications

(39 citation statements)

References 50 publications

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“… 36 Ruminococcus 1 is a highly heterogeneous genus in which some species have probiotic effects, 36 whereas others are considered pathogenic. A prior study found a positive correlation between the abundance of Romboutsia and obesity, 37 suggesting that these bacteria play a negative role in lipid metabolism. Interestingly, Faecalibaculum , a genus believed to promote the beneficial generation of SCFAs in the intestines, 38 had an increased relative abundance in NAFL/NASH mice, as did Bifidobacterium , a genus that has long been considered a probiotic.…”

Section: Discussionmentioning

confidence: 99%

Using intestinal flora to distinguish non-alcoholic steatohepatitis from non-alcoholic fatty liver

Cui

Wang

et al. 2020

J Int Med Res

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Objective To explore specific flora in mouse models of non-alcoholic steatohepatitis (NASH) to improve NASH diagnostic protocols. Methods Sixty mice were divided into normal diet (ND, 20 mice) and high-fat/high-sugar diet (HFSD) groups (40 mice). After 8 weeks of feeding, 10 mice in the ND group and 20 mice in the HFSD group were sacrificed to create the short-term ND and non-alcoholic fatty liver (NAFL) groups, respectively. After 16 weeks of feeding, the remaining mice were sacrificed to create the long-term ND and NASH groups, respectively. We then examined fecal flora, serum biochemical indices, and lipopolysaccharide and tumor necrosis factor-α levels and analyzed liver tissue. Results The relative abundance of Lactobacillus, Desulfovibrio, Ruminiclostridium 9, and Turicibacter differed between NASH and NAFL mice, and the areas under the receiver operating characteristic curve of the four genera for diagnosing NASH were 0.705, 0.734, 0.737, and 0.937. The non-alcoholic fatty liver disease activity score was positively correlated with the relative abundance of Desulfovibrio (r = 0.353), Ruminiclostridium 9 (r = 0.431), and Turicibacter (r = 0.688). Conclusions The relative abundance of Lactobacillus, Desulfovibrio, Ruminiclostridium, and Turicibacter may help distinguish NASH from NAFL.

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Section: Discussionmentioning

confidence: 99%

Using intestinal flora to distinguish non-alcoholic steatohepatitis from non-alcoholic fatty liver

Cui

Wang

et al. 2020

J Int Med Res

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“… Zeng et al (2019) performed a clinical study and found that Blautia , Romboutsia , Ruminococcus , and Dorea were positively correlated with indicators of bodyweight and serum lipids. In addition, Hu et al (2019) showed that both Romboutsia and Desulfovibrio were obesity-related bacteria. Li et al (2018) reported a significant relationship between Romboutsia and serum TC, TG, and LDL-C levels.…”

Section: Discussionmentioning

confidence: 99%

The Therapeutic Efficacy of Curcumin vs. Metformin in Modulating the Gut Microbiota in NAFLD Rats: A Comparative Study

Yao

Gao

et al. 2021

Front. Microbiol.

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Structural disruption of gut microbiota is closely related to the occurrence of non-alcoholic fatty liver disease (NAFLD). Previous research has demonstrated that both curcumin (CUR) and metformin (MET) have a therapeutic effect against NAFLD and play a role in modulating the gut microbiota. However, there is a lack of direct comparison between the two medications in terms of the therapeutic efficacy and the regulatory effect on gut microbiota. In this study, we administered either CUR or MET to rats with high-fat diet (HFD)-induced obesity to observe changes in body parameters, biochemical parameters, liver, and ileum pathology and gut microbiota, and used next generation sequencing and multivariate analysis to evaluate the structural changes of gut microbiota in a NAFLD rat model before and after CUR and MET intervention. It was found that both CUR and MET attenuated hepatic ectopic fat deposition, alleviated inflammatory factors, and improved intestinal barrier integrity in HFD-fed rats. More importantly, CUR and MET reduced the Firmicutes/Bacteroidetes ratio and reverted the composition of the HFD-disrupted gut microbiota. Both CUR and MET treatments effectively modified the gut microbiome, enriched the abundance of beneficial bacteria and reduced opportunistic pathogens in obese rats. The abundance of Butyricicoccus was increased while the abundance of Dorea was decreased in HFD + CUR group. Besides, some beneficial bacteria such as Prevotella were increased in MET-treated animals. Spearman’s correlation analysis showed that Helicobacter, Akkermansia, Desulfovibrio, Romboutsia, Corynebacterium, Lactobacillus, Ruminococcaceae_unclassified, Lachnospiraceae_unclassified, and Clostridiales_unclassified showed significantly positive correlations with TG, TC, LDL-C, GLU, IL-6, IL-1β, and TNF-α, and negative correlations with HDL-C (both p < 0.05). However, Prevotella and Stomatobaculum showed an opposite trend. In summary, CUR and MET showed similar effects in alleviating hepatic steatosis, improving intestinal barrier integrity and modulating gut microbiota in HFD-induced obesity rats, and therefore may prove to be a novel adjunctive therapy for NAFLD.

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“…Among these is Ruminiclostridium_ 9, a bacterial group which has been previously connected to obesity and other metabolic disorders ( Wang et al, 2019 ), although these results are regarded with caution. While some studies have proposed that obese mice have a higher abundance of Ruminiclostridium_9 in their fecal microbiota than the leaner counterparts ( Hou et al, 2019 ; Luo et al, 2019 ; Wang et al, 2020 ), others have shown that Ruminiclostridium_9 is instead negatively correlated with obesity ( Zhao et al, 2017 ; Zhu et al, 2018 ; Hu et al, 2019 ). Another example of a MSTN-edited upregulated group in the cecum is Succinivibrio , a bacteria that has high potential for fiber degradation ( Hippe et al, 1999 ) and thus can improve metabolism.…”

Section: Discussionmentioning

confidence: 99%

Integrated Microbiome and Metabolome Analysis Reveals a Positive Change in the Intestinal Environment of Myostatin Edited Large White Pigs

Pei

Chen

et al. 2021

Front. Microbiol.

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Myostatin (MSTN) functional inactivation can change the proportion of lean meat and fat content in pigs. While both genotype and microbial composition are known to affect the host phenotype, so far there has been no systematic study to detect the changes in the intestinal microbial composition and metabolome of MSTN single copy mutant pigs. Here, we used 16S rDNA sequencing and metabolome analysis to investigate how MSTN gene editing affects changes in the microbial and metabolome composition in the jejunum and the cecum of Large White pigs. Our results showed that Clostridium_sensu_stricto_1, Bifidobacterium, Lachnospiraceae_UCG-007, Clostridium_sensu_stricto_6, Ruminococcaceae_UCG-002, and Ruminococcaceae_UCG-004 were significantly upregulated; while Treponema_2 and T34_unclassified were significantly downregulated in the jejunum of MSTN pigs. Similarly, Phascolarctobacterium, Ruminiclostridium_9, Succinivibrio, Longibaculum, and Candidatus_Stoquefichus were significantly upregulated, while Barnesiella was significantly downregulated in the cecum of MSTN pigs. Moreover, metabolomics analysis showed significant changes in metabolites involved in purine, sphingolipid and tryptophan metabolism in the jejunum, while those associated with glycerophospholipid and pyrimidine metabolism were changed in the cecum. Spearman correlation analysis further demonstrated that there was a significant correlation between microflora composition and metabolites. Our analyses indicated the MSTN editing affects the composition of metabolites and microbial strains in the jejunum and the cecum, which might provide more useable nutrients for the host of MSTN± Large White pigs.

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Long-Chain Bases from Sea Cucumber Alleviate Obesity by Modulating Gut Microbiota

Cited by 48 publications

References 50 publications

Using intestinal flora to distinguish non-alcoholic steatohepatitis from non-alcoholic fatty liver

Using intestinal flora to distinguish non-alcoholic steatohepatitis from non-alcoholic fatty liver

The Therapeutic Efficacy of Curcumin vs. Metformin in Modulating the Gut Microbiota in NAFLD Rats: A Comparative Study

Integrated Microbiome and Metabolome Analysis Reveals a Positive Change in the Intestinal Environment of Myostatin Edited Large White Pigs

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