Long-Chain Acyl-CoA Synthetases Promote Poplar Resistance to Abiotic Stress by Regulating Long-Chain Fatty Acid Biosynthesis

Abstract

Long-chain acyl-CoA synthetases (LACSs) catalyze fatty acids (FAs) to form fatty acyl-CoA thioesters, which play essential roles in FA and lipid metabolisms and cuticle wax biosynthesis. Although LACSs from Arabidopsis have been intensively studied, the characterization and function of LACSs from poplar are unexplored. Here, 10 poplar PtLACS genes were identified from the poplar genome and distributed to eight chromosomes. A phylogenetic tree indicated that PtLACSs are sorted into six clades. Collinearity analysis and duplication events demonstrated that PtLACSs expand through segmental replication events and experience purifying selective pressure during the evolutionary process. Expression patterns revealed that PtLACSs have divergent expression changes in response to abiotic stress. Interaction proteins and GO analysis could enhance the understanding of putative interactions among protein and gene regulatory networks related to FA and lipid metabolisms. Cluster networks and long-chain FA (LCFA) and very long-chain FA (VLCFA) content analysis revealed the possible regulatory mechanism in response to drought and salt stresses in poplar. The present study provides valuable information for the functional identification of PtLACSs in response to abiotic stress metabolism in poplar.

Chromosome‐level genome assembly of Pedicularis kansuensis illuminates genome evolution of facultative parasitic plant

“…evm.model.CTG_1914.20, evm.model.CTG_2264.34, and evm.model. CTG_2192.44 have been identified to play significant roles in salt tolerance (Jia et al, 2023;Li et al, 2015;Wei et al, 2022). Additionally, the homologous genes of evm.model.CTG_2192.44 in rice have been linked to flooding tolerance (He et al, 2014).…”

“…For instance, the homologous genes of evm.model.CTG_1235.41 and evm.model.CTG_1914.20 have been found to be involved in responses to drought stress (Ni et al., 2021; Xue et al., 2017). Similarly, the homologous genes of evm.model.CTG_1914.20 , evm.model.CTG_2264.34 , and evm.model.CTG_2192.44 have been identified to play significant roles in salt tolerance (Jia et al., 2023; Li et al., 2015; Wei et al., 2022). Additionally, the homologous genes of evm.model.CTG_2192.44 in rice have been linked to flooding tolerance (He et al., 2014).…”

Insights into the Jasmonate Signaling in Basal Land Plant Revealed by the Multi-Omics Analysis of an Antarctic Moss Pohlia nutans Treated with OPDA

“…In the present study, we found that most of C18 fatty acids and VLCFAs (i.e., 12-oxo-5,8,10,14-eicosatetraenoic acid, 13 S -hydroperoxy-9 Z ,11 E -octadecadienoic acid, 15-oxo-5 Z ,8 Z ,11 Z ,13 E -eicosatetraenoic acid, and 2 R -hydroxy-9 Z ,12 Z ,15 Z -octadecatrienoic acid) were markedly downregulated under OPDA treatment ( Figure 2 ). The carbon chain length, unsaturation, and head structure polarity of VLCFAs make them extremely diverse in structure and function and play a key role in transmembrane transport, cell division and differentiation, energy storage, resistance to stress, and other physiological processes [ 42 , 44 ]. Our result showed that lots of markedly upregulated or downregulated family genes (i.e., 3-ketoacyl-CoA synthase, 3-ketoacyl-CoA thiolase, and long-chain acyl-CoA synthetase) involved in phospholipids biosynthesis in P. nutans ( Figure 6 and Table S2 ).…”